Induction of systemic resistance against damping-off and root-rot of white lupine (Lupinus albus L.) using some bioagents, chemical inducers and a mycorrhizal fungus

Document Type : Research Paper

Authors

1 Plant Pathology Research Institute, Agriculture Research Centre, 12619, Giza, Egypt

2 El Nelain University, Faculty of Science and Technology, Khartoum, Sudan

3 Plant Pathology Department, Faculty of Agriculture, Cairo University, Egypt

10.22124/cjes.2022.5693

Abstract

Damping-off and root-rot diseases of lupine are considered the main problem in lupine production in Egypt. In this study, the effect of two bioagents i.e., Trichoderma harzianum and Bacillus megaterium, two chemical inducers i.e., salicylic and citric acid, and mycorrhizal fungus compared to the fungicide Topsin-M70 were tested in vitro, greenhouse and field conditions against Fusarium exysporium, F. Solani, and Rhizoctonia solani are the causes of root rot and damping-off of lupine plants. All of the tests were done in vitro. The tested linear growth of bacteria was significantly slowed by bioagents of the three pathogenic fungi, B. megaterium. The most significant reduction was induced by T. harzianum, which was followed by T. harzianum. Sowing lupine in the seeds soil artificially inoculated with any of the three pathogenic fungi with a large increase in fresh and dry weight. The incidence of pre-and post-emergence damping-off was significantly reduced compared to the control treatment. B.megaterium and Topsin-M70 exhibited the highest percentages of survived plants. In general, in all the tested bio-agents, chemical inducers, the mycorrhizal fungus, and Topsin-M70 under field circumstances, the incidence of damping-off was dramatically reduced. The data obtained revealed all the tested treatments. Peroxidase, polyphenol oxidase, and chitinase enzymes, which play critical roles in plant metabolism, saw a significant increase inactivity, defense mechanisms that work against pathogens infection.                                                                                  

Keywords

References

Abd Allah, ES, Hashem, A, Algarawi, AA & Alwathnani, HA 2015, Alleviation of adverse impact of cadmium stress in sunflower (Helianthus annvus L.) by arbuscular mycorrhizal fungi. Pakistan Journal of Botany, 47: 785-795.
Abd El Khair, HR, Khalifa, KhM & Haggag, KHE 2011, Effect of Trichderma species on damping–off diseases incidence, some plant enzymes activity and nutritional status of bean plants. Journal of American Science, 7: 156-167.
Abd El Ghany, M 2007, Pathological studies on Apricot Root-rot in the New Reclaimed Lands in Egypt. PhD Dissertation, Faculty of Agriculture, Al-Azhar University, 82 p.
Aboelmagd, HE, Mohamed, FG, Eid, KhE & El Fiki, IAI 2021, Efficacy of some bio-agents, chemical inducers and fungicides in controlling tomato root-rot disease caused by Rhizoctonia solani. 5th International Conference on Biotechnology Applications in Agriculture, (ICBAA), Benha University, 8 April, Egypt (Conference Online), 197-210.
Abo Rehab, ME 1997, Studies on Root-rot of Persimmon and its Control in the New Reclaimed in Egypt. MSc. Dissertation, Faculty of Agriculture, Cairo University, 94 p.
Ahmed, GA 2011, Induction Resistance of Cucumber Plant (Cucumber sativus L.) against Fusarium Wilt Disease under Protected Houses conditions. PhD Dissertation, Kazakh National Agrarian University, 151 p.
Akhter, A, Hage Ahmed, K, Soja, G & Steinkellner, S 2015, Compost and biochar alter mycorrhization tomato root exudation and development of Fusarium oxysporum f. sp. lycopersici. Frontiers in Plant Science, 6: 529. http://doi.org/10.3389/fpls.2015.00529.
 Algarawi, AA,  Abd Allah, FF & Hashem, A 2014, Alteration of salt induced adverse impact via mycorrhizal fungi in Ephedra aphylla Forssk. Journal of Plant Interactions, 1: 802-810, http://www. tandfonline.com/ foi/tJpi20.
Al Hmoud, G & Al Momany, AJ 2015, Effect of four mycorrhizal products on Fusarium root- rot different vegetable crops. Journal of Plant Pathology & Microbiology, 6: 2 https://dio.org/10.417212157-7471.1000255.
Akhtar ME & Siddiqui, ZA 2008, Glomus intraradices, Pseudomonas alcaligenes, and Bacillus pumilus: effective agents for the control of root-rot disease complex of chickpea (Cicer arietinum L.). Journal of General Plant Pathology, 74:53–60. DOI: 10.1007/s10327-007-0062-4.
Asaka, O & Shoda, M 1996, Bio-control of Rhizoctomia solani damping–off of tomato with Bacillus subtillus RB14. Applied and Environmental Microbiology, 62: 4081- 4085.
Boller, T & Mauch, F 1988, Colorimetric assay for chitinnase. Methods in Enzymology, 161: 430-435.
de Melol, IS & Faull, JL 2000, Parasitism of Rhizoctonia solani by strains of Trichoderma spp. Scientia Agricola, 57: 1.
Demir, S & Akkopru, A 2007, Use of arbuscular mycorrhizal fungi for biocontrol of soil-borne fungal plant pathogens. In: SB Chincholkar, KG, Mukerji (eds.), Biological control of plant diseases. Howarth, New York, 17-37.
El Mohamedy, RS & Abdel Alla, MA 2013, Bio-primary seed treatment for biological control of soil borne fungi causing root-rot of green bean (Phaseolus vulgaris L.). Journal of Agricultural Science and Technology, 9: 589-599.
El Mohamedy, RS, Shafeek, MR & Rizk Fatma, A 2015, Management of root-rot diseases and improvement growth and yield of green bean plants using plant resistance inducers and biological seed treatments. Journal of Agricultural Science and Technology, 11: 1219-1234.
El Gendy Hala, M, El Sayed Sahar, A & Mosa, AM 2016, Efficacy of two natural extracts and fungicides in reducing soybean damping-off and Root –rot disease Egypt. Journal of Phytopathology, 44: 35-50.
El Sayed Sahar, A 2006, Use of Intercropping and other Treatments for Controlling Faba-bean Diseases. PhD Dissertation, Faculty of Agriculture, Benha University, Egypt.
El Sayed Sahar, A, El Gendy Hala, M & Abd El Motaleb, FA 2015, Efficiency of some micro-element root-rot disease yield characters and chemical composition of lupine. Journal of Biological Chemistry and Environmental Sciences, 10: (625-640).
Fu, J & Huang, B 2001, Involvement of antioxidants and lipid peroxidation in the adaptation of two cool-season grasses to localized drought stress. Environmental and Experimental Botany, 45: 105-114.
Julier, B, Wuyghe, C & Papineall, J 1994, Dry matter and nitrogen accumulation in determinate autumn-sown white lupine (Lupinus albus) c.v. lunoble. European Journal of Agronomy, 3: 153-160.
Katan, J 2004, Soil Disinfestation: One minute before methyl bromide, phase out. 4th International Symposium on Chemical and Non-chemical Soil and substrate Disinfestation, 698: 19 -26.
Kessmann, H, Sataub, T, Hofmann, C, Meatzke, T & Herzog, J 1994, Induction of systemic acquired disease resistance in plants by chemicals. Annual Review of Phytopathology, 32: 439-459.
Kosuge, T 1996, The role of phenolics in host response to infection to infection. Annual Review of Phytopathology, 7:195–222.
Manzali, D, Nipoti, P, Pisi, A, Filippini, G & D'Ercole, N 1993, Scanning electron microscopy study of in vitro antagonism of Trichoderma spp. strains against Rhizoctonia solani Kühn. Phytopathologia Mediterranea, 32: 1-6 .
Mayer, AM, Harel, E & Shaul, RB 1965, Assay of catechol oxidase a critical comparison of methods. Phytochemistry, 5:783-789.
Maslienko, LV, Voronkova, AK, Datsenko, LA & Efimtseva, EA 2021, Antagonistic effect of the promising fungal producer strain of microbiopreparation T-1 Trichoderma sp. on oil flax Fusarium blight. Caspian Journal of Environmental Sciences, 19: 883-890
Melo, GA, Shimizu, MM & Mazzafera, P 2006, Polyphenol oxidase activity in coffee leaves and its role in resistance against the coffee leaf miner and coffee leaf rust. Phytochemistry, 67: 277-285.
Metranx, JD & Boller, T 1986, Local and systemic induction of chitinase in cucumber plants in response to fungal, bacterial and viral infections. Physiological and Molecular Plant Pathology, 28: 161-169
Miljakovic, D, Marinkovic, J & Balesevic Tubic, S 2020, The significance of Bacillus spp. in disease suppression and growth promotion of field and vegetable crops. Microorganisms, 8: 1037. DOI: 10.3390/ microorganisms8071037.
Morkunas, I & Gemerek, J 2007, The possible involvement of peroxidase in defence of yellow lupine embryo axes against Fusarium oxysporum. Journal of Plant Physiology, 164: 497-506.
Nahiyan, ASM & Matsubara, Y 2012, Tolerance Fusarium root-rot and changes in antioxidative ability in mycorrhizal asparagus plants. Horticultural Science, 47: 356-360.
Noval, AM, Abd El Rahman, M, Abdelghany, TM & Abd El Mongy, M 2020, Mycoparasitic nature of Egyptian Trichoderma isolates and their impact on suppression Fusarium wilt of tomato.  Egyptian Journal of Biological Pest Control, 31: 103. 
Ragab, MMM, Abada, KA, Abd El Moneim, ML & Abo Shosha, YZ 2015, Effect of different mixtures of some bioagents and Rhizobium phaseoli on bean damping-off under field condition. International Journal of Scientific and Engineering Research, 6: 1009-1106.
Salt, GA 1982, Factors affecting resistance to root rot diseases (Eds. G, Hawtin, & C, Webb) faba bean improvement. IARDA, Aleppo, Syria, 260-270.
Shalaby, SIM 1997, Effect fungicidal treatment of sesame seeds on root-rot infection, plant growth and chemical components. Bulletin of Faculty of Agriculture, Cairo University, 48: 397-411.
Shaltout, AMA 2002, Use mycorrhizal fungi and some other bioagents to control root-rot and stalk-rot of corn. PhD Dissertation, Faculty of Agriculture, Cairo University, 127 pp.
Shevchenko, VA, Soloviev, AM, Popova, NP 2021 Eligibility criteria for joint ensilage of maize and yellow lupine on poorly productive lands of the Upper Volga region. Caspian Journal of Environmental Sciences, 19: 745-751
Siddiqui ZA, Iqbal, A & Mahmood, I 2001, Effects of Pseudomonas fluorescens and fertilizers on the reproduction of Meloidogyne incognita and growth of tomato. Applied Soil Ecology, 16: 179-185.
Snedecor, GW & Cochran, WG 1989, Statistical methods. Oxford and J. pH. Publishing Cam. 8th edition.
Virale, F, Marra, R, Scala, F, Ghisalberti, E, Lorito, M & Sivasitham Param, K 2006, Major secondary metabolites produced by two commercial Trichoderma strains active against different phytopathogens. Letters in Applied Microbiology, 43: 143-148.
Zian, AH 2011, Studies on Fusarium Wilt Disease of White lupine Plant in Egypt. PhD Dissertation, Faculty of Agriculture - Suez Canal University, Egypt, 167 p.
Caspian Journal of Environmental Sciences
Volume 20, Issue 3
July 2022
Pages 571-583

Files

Share

How to cite

Statistics