Deposit of indoxasacarb as a residue in the organs of pregnant rats and its influence on the activity of transferase enzymes

Document Type : Reviewers

Authors

1 Department of Biology and Geography, Andijan State Pedagogical Institute, Andijan, Uzbekistan

2 Department of Botany and Ecology, National Pedagogical University of Uzbekistan named after Nizami, Tashkent, Uzbekistan

3 Department of Biophysics, National University of Uzbekistan named after M. Ulugbek, Tashkent, Uzbekistan

4 Department of Zoology and Anatomy, National Pedagogical University of Uzbekistan named after Nizami, Tashkent, Uzbekistan

5 Department of Biochemistry and Physiology, Tashkent State Agrarian University, Tashkent, Uzbekistan

6 Department of Chemical Sciences, Rector of Chirchik State Pedagogical University, Chirchik, 110700, Uzbekistan

7 Department of Inorganic, Physical and Colloidal Chemistry, Tashkent Pharmaceutical Institute, Tashkent, Uzbekistan

8 Department of Chemical Sciences, Academy of Sciences of Uzbekistan of the Institute of the Chemistry Plant of Substances, Tashkent, Uzbekistan

9 Nukus State Technical University, Nukus, Uzbekistan

10 Bukhara State Medical Institute named after Abu Ali ibn Sino, Bukhara, Uzbekistan

10.22124/cjes.2026.9966

Abstract

This article analyzes the residual content of the pesticide indoxacarb in the tissues of certain organs of pregnant rats and their embryos. Also, the influence of safaroflavonoside (SFL) isolated from the plant Crocus sativus L. and narsissin flavonoids isolated from the plant Alhagi canescens on the content of transferase A enzymes and bilirubin in the blood plasma of pregnant rats intoxicated with the pesticide indoxacarb was studied. The pesticide Indoxacarb was administered into the stomach of pregnant rats by the method of per os through a special probe at a dose of LD50 1/10 on days 3, 13, and 17 of pregnancy. On the 20th day of pregnancy, the determination of the amount of residual pesticides in the liver, spleen, brain, and lung tissues of female rats and their embryos was carried out using the LC-MS (China) device. Residual pesticide levels were determined in the liver, spleen, brain, and lung tissues of pregnant rats. A high content of residual pesticide was observed in the liver tissue. In the organs of the embryos of pregnant rats, the presence of the rumen pesticide was also detected. It was found that the residual pesticide content in the liver tissues of pregnant rats and their embryos is higher than in other organs.
On the 3rd and 13th days of pregnancy, rats were subjected to single poisoning with the pesticide indoxacarb at a dose of 1/10 LD50 , and narcissin and SFL flavonoids were administered orally at a dose of 10 mg/kg for 7 days from that day. Experimental rats were divided into group IV. It was found that flavonoid compounds reduced the levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), and total bilirubin in pregnant rats poisoned with indoxacarb.

Keywords

References

Anthony, OB & Omoniyi, FO 2022, Determination of pesticide residues in muscle and organs of cow, camel and goat in Birnin Kebbi, Kebbi State, Nigeria. International Journal of Environmental Sciences, 5(2): 33–56.
Araoud, M, Neffeti, F, Douki, W, Hfaiedh, HB, Akrout, M, Hassine, M & et al. 2012, Adverse effects of pesticides on biochemical and hematological parameters in Tunisian agricultural workers. Journal of Exposure Science & Environmental Epidemiology, 22(3): 243–247.
Approved Recommendation 1985, Recommendation on IFCC methods for the measurement of catalytic concentration of enzymes. Part 3: IFCC method for alanine aminotransferase (EC 2.6.1.2). Journal of Clinical Chemistry and Clinical Biochemistry, 24.
Bossi, R, Vinggaard, AM, Taxvig, C, Boberg, J & Bonefeld-Jørgensen, EC 2013, Levels of pesticides and their metabolites in Wistar rat amniotic fluids and maternal urine upon gestational exposure. International Journal of Environmental Research and Public Health, 10: 2271–2281.
Brown, JB, Langley, SA, Snijders, AM & et al. 2021, An integrated host-microbiome response to atrazine exposure mediates toxicity in Drosophila. Communications Biology, 4: 1324.
Cao, L, Kang, Q & Tian, Y 2021, Pesticide residues: Bridging the gap between environmental exposure and chronic disease through omics. Ecotoxicology and Environmental Safety, 287: 117335. DOI: 10.1016/j.ecoenv. 2024.117335.
Dwivedi, N, Flora, G, Kushwaha, P & Flora, SJ 2014, Alpha-lipoic acid protects oxidative stress, changes in cholinergic system and tissue histopathology during co-exposure to arsenic-dichloros in rats. Environmental Toxicology and Pharmacology, 7: 23.
Espanola, QS, Científico, C & Enzimas, C 1987, Method recommended for determining the catalytic concentration of aminotransferred alanine in human blood. Quimica Clinica, 6: 241–244.
Ferguson, KK, van den Dries, MA, Gaillard, R, Pronk, A, Spaan, S, Tiemeier, H & Jaddoe, VW 2019, Exposure of organophosphate pesticide in pregnancy in association with ultrasound and delivery measures of fetal growth. Environmental Health Perspectives, 127: 87005.
Gella, FJ, Olivella, T, Cruz Pastor, M, Arenas, J, Moreno, R, Durban, R & Gómez, JA 1985, A simple procedure for routine determination of aspartate aminotransferase and alanine aminotransferase with pyridoxal phosphate. Clinica Chimica Acta, 153: 241–247.
Lasch, A, Marx-Stoelting, P, Braeuning, A & Lichtenstein, D 2021, More than additive effects on liver triglyceride accumulation by combinations of steatotic and non-steatotic pesticides in HepaRG cells. Archives of Toxicology, 95: 1397–1411.
Mahmoud, AFA, Ikenaka, Y, Yohannes, YB, Darwish, WS, Eldaly, EA, Morshdy, AEMA, Nakayama, SMM, Mizukawa, H & Ishizuka, M 2016, Distribution and health risk assessment of organochlorine pesticide (OCPs) residue in edible cattle tissues from northeastern part of Egypt: High accumulation level of CPs in language. Chemosphere, 144: 1365–1371.
Matthews, GA 2015, Pesticides-health, safety and the environment, 2nd edn. Wiley Blackwell, DOI: 10.1002/ 9781118975923, ISBN: 978-1-118-97586-2
Megharaj, M, Ramakrishnan, B, Venkateswarlu, K, Sethunathan, N & Naidu, R 2011, Bioremediation approaches for organic pollutants: A critical perspective. Environment International, pp. 135–137.
Mehta, P, Parajuli, RP, Chronister, BNC, Yang, K, Barr, DB, Tu, XM, Lopez-Paredes, D & Suarez-Lopez, JR 2025, Pesticide and liver biomarkers among Ecuadorian adolescents and adults living in agricultural settings. Toxics, 13: 685.
Mirkhamidova, P, Parpieva, M & Tuychieva, D 2021, Residual pesticide in the liver of rats after poisoning with galaxifop-R-methyl pesticide check. International Journal of Modern Agriculture, 10(2): 2457–2465.
Mirkhamidova, P, Pozilov, MK, Parpieva, MJ, Shakhmurova, GA & Jumagulova, KA 2022, Determination of the amount of residual pesticides in the liver tissue of rats poisoned with haloxyfop-R-methyl and indoxacarb pesticides. Journal of Pharmaceutical Negative Results, 13(Special Issue 1): 738–745.
Munir, S, Azeem, A, Zaman, MS & Haq, MZU 2021, From field to table: Ensuring food safety by reducing pesticide residues in food. Science of the Total Environment, 922: 1–10.
Parpieva, MJ, Mirkhamidova, P, Pozilov, MK & Alimova, RA 2023, Effects of sophoroflavonoside and narcissin flavonoids on the amount of malondialdehyde, a product of lipid peroxidation, and the activity of the enzyme cytochrome c oxidase in rat liver mitochondria poisoned with the pesticide indoxacarb. Caspian Journal of Environmental Sciences, 21(4): 997–1000. https://doi.org/10.22124/CJES.2023.7179
Parpieva, MJ, Mirkhamidova, P, Pozilov, MK & Alimova, RA 2023, Effects of sophoroflavonoside and narcissin flavonoids on the amount of malondialdehyde, a product of lipid peroxidation, and the activity of the enzyme cytochrome c oxidase in rat liver mitochondria poisoned with the pesticide indoxacarb. Caspian Journal of Environmental Sciences, 21(4): 997–1000.
Xu, MY, Wang, P, Sun, YJ, Wang, HP, Liang, YJ, Zhu, L & Wu, YJ 2015, Redox status in the liver of rats after subchronic exposure to the combination of low-dose dichlorvos and deltamethrin. Pesticide Biochemistry and Physiology, 124: 60–65.
Caspian Journal of Environmental Sciences
Volume 24, Issue 4
October 2026
Pages 1279-1287

Files

Share

How to cite

Statistics