Isolation and Optimization of Tobacco Decomposing Bacillus and Lactobacillus Sp.


Environmental Microbiology Laboratory, Department of Zoology, University of the Punjab, Quaid-e-Azam Campus, Lahore 54590, Pakistan


Three bacteria from a tobacco industry effluent and one from a sewage sample were isolated on a medium comprising of 1% tobacco powder as sole source of nutrients. Bacteria isolated from the industrial waste water were identified as Bacillus cereus, B. alvei and B. circulans. While, Lactobacillus sp. was isolated from the sewage sample. The bacteria were optimized for various growth conditions in a medium comprising of aqueous extract of 1% tobacco powder in MIII. Provision of 0.1% molasses (MIII) enhanced growth of B.cereus as compared to its cultivation in MII. Growth of B.cereus up to two weeks in molasses supplemented 1% tobacco powder (MIV) medium resulted into retroprogressive decreases in total suspended matter, ash content and total organic matter. So that at the last sampling period these parameter reduced up to 92%, 82% and 42%, respectively as compared to control values. The bacterial isolates in general and the B.cereus practically appear promising for designing in situ biotreatment plants for tobacco processing units and are likely to add in reducing environmental contamination originating from tobacco allied toxic compounds.
Bejankiwar, R.S. (2002) Electrochemical treatment of cigarette industry waste water feasibility study. Water. Res. 36, 4386-4390
Campbell, R.C. (1989). Statistics for biologist. Cambridge University Press Cambridge.
Civilini, M, Domenis, C, Sebastianutto, N, Bertoldi, M. (1997). Nicotine decontamination of tobacco agro-industrial waste and its degradation by microorganisms’ Waste. Manag . Res. 15, 349-358
 Clarke, A.B., Stanley, J. (1964). Process for selective extraction of alkaloid. United States Patent No 3, 319-435
De Lucas, A, Canizares, P, Garcia, M.A, Gomez, J, Rodrigez, J.F. (1998) .Recovery of nicotine from aqueous extracts of tobacco wastes. Indust. Enging. Chem. Res .37, 4783-4791.
 Gorrod, J.W ,Jacob, P. (1999). Analytical determination of nicotine and related compounds and their metabolites Part. Environ. Tobacco. Products. ISBN: 0-444-50095-2, Elsevier, 732 p.
Gravely, L.E, Geiss, V.L, Newton, R.P. (1977). Process for maximizing the growth and nicotine degrading activity of microorganisms United States Patent No .4.011.141
Gupta, P.K. (2000). Methods in environmental analysis water soil and air .AGROBIOS (INDIA) Behind Nasarani Cinema Chopasani Road Jidhpur.
Holt, J.G, kriey, N.R, Sneath, P.H.A, Staley, J.T, Williams, S.T.(1994). Bergeys´s manual of determinative bacteriologyLippincott Williams and Wilkins, Philadelphia, USA. pp. 562-568
Ireland, M.S, Larson, T.M , Moring, T.M. (1980). Nicotine transfer process. United States Patent No 4.215.706
Konenan ,E.W, Allen, S.D. Janda, W.M. Schree, Kenberger ,P.C. Winn, Jr. W.C. (1997). Lippincot Williams and Wilkins 530 Walnur street, Philadeiphia, PA 19106 -3621 USA.
Meher, K.K, Panchwagh, A.M, Rangrass, S ,Gollakota, K.G. (1995). Biomethanation of Tobacco. Waste. Environ. Pollut. 90, 199–202.
Munari, M. (1986). Quantitative Determination of Nicotine Content in Protein Extracted from Tobacco.Tobacco Journal International, 2, 128– 132.
Saunders, J.A , Blume, D.E. (1981). Quantitation of major tobacco alkaloids by high performance liquid chromatography. Chromatog. 205, 147–154.
Sax, N.I, Lewis, R.J. (1989). Dangerous properties of industrial materials. New York USA:248
Sponza,D.T. (2002). Toxicity studies in a tobacco industry biological treatment plant. Water, Air and Pollution, 134, 137-164.