Assessment of Two Different Sources of Durable Resistance and Susceptible Cultivar of Wheat to Stripe Rust (Puccinia striiformis f. sp. tritici)

A study was conducted to assess the durable resistance in a near isogenic line of spring wheat (Triticum aestivalis L.), possessing resistance gene Yr-18 to some isolates (race specific resistance) of stripe rust (Puccinia striiformis f. sp. tritici), namely Thatcher Yr-18 and durable resistance of an cultivar of spring wheat to all isolates of stripe rust (race non- specific resistance), namely Hybrid de Berse'e. In this investigation fresh urediniospores of two isolates namely SRC 99 (race 70E 128) and SRC 89 (race 14E 14), were collected from a susceptible cultivar of spring wheat, Avocet, as inoculums. Then suspension of spore in mineral oil [Soltrol 170 (5 mg/ml)] were sprayed on third and flag leaves of the booth genotypes mentioned above and on the Thatcher cultivar which was susceptible to booth isolates. The Percentage of urediniospore germination, latent period and infection types of all susceptible and resistant genotypes to booth isolates were determined in four replicates at seedling and adult plant stages. Mean percentage of spore germination and the value of latent period were analyzed separately in each replicate, and then compared using analysis of variance and the Student- Newman Keuls test. The results indicated that urediniospoe germination in all genotypes / isolates/ leaf stage combination was high and neither resistance type affected spore germination significantly. In both types of resistance, latent period increased with advancing plant age. In general cultivars possessing resistant gene had longer latent periods. Assessment of the infection type showed that, the durable resistance of Hybrid de Bers?e is detected at an earlier stage than the adult-plant resistance of near isogenic line containing the gene Yr 18.
 
REFERENCES
Bamdadian, A. (1972) Physiological races of Puccinia striiformis in Iran. Proceeding of the European and Mediterranean Cereal Rust Conference. Prague, pp. 91- 95.
Biffen, R. H. (1905) Mendel's laws of inheritance and wheat breeding. Journal of Agricultural Science 1, 4- 48.
Biffen, R. H. (1912) Studies in inheritance in disease resistance. II. Journal of Agricultural Science 4, 412- 419.
Coner, R. L. and Kuzyke, A. D. (1988) Effectiveness of different fungicides in controling stripr, leal rust, and black point. Can. J. Plant pathology 73, 927- 936.
Elahinia, S. A. (2000) Assessment of urediniospore germination of Puccinia striiformis at various temperature on agar and detached leaves of wheat. Journal of Agriculture, Science and Technology 2, 1-8.
Elahinia, S. A. (1989) Resistance of Wheat to Puccinia striiformis. Ph.D Dissertation, University of Salford, Manchester, UK. Gassner, G. and Streib, W. (1932) Die Bestimmung der Biologischen Rassen des Weizengelbrostes (Puccinia glumarum f. sp. Tritici) (Schem.) Erikss. U. Henn. Arb. Biol. Reich Anst. Land-u. Forestw. 20, 121- 145.
Goddard, M. V. (1974) Induced variation in Puccinia striiformis and studies of related to cytological developmental processes. Ph.D. Dissertation, Cambridge University. UK. Johnson, R. and Law, C. N. (1973) Cytogentic studies on the resistance of wheat variety Berseé to Puccinias triiformis .Cereal Rusts Bulletin, 81, 38- 43.
Johnson, R. and Law, C. N. (1975) Genetic control of durable resistance to yellow rust (Puccinia striiformis) in the wheat cultivar hybrid de Bersée. Annals Applied Biology 81, 385- 391.
Johnson, R. (1981) Durable disease resistance: definition of genetic cotrol and attainment in plant breeding. Pytolopathology 71, 567- 568.
Khazra, H. and Bamdadian, A. (1974) The wheat disease situation in Iran. Proceeding of Fourth FAO/Rockfller Foundation Wheat Seminar, Tehran. Iran, pp. 292- 299.
McIntosh, R. A., G. E. Hart, K. M. Devos, M.D. Gale , and W. J. Rogers (1998). Catalogue of gene symbols for wheat. Proceed. 9th int. Wheat genet. Symp. Vol. 5: 236 p.
Mares, D. J. and Cousen, S. (1977) The interaction of yellow rust (Puccinia striformis) with winter wheat cultivars showing adult plant resistance: macroscopic and microscopy event associated with the resistant reaction. Physiological Plant Pathology 10, 257-274.
Parlevliet, J. E. (1975). Partial resistance of barley to leaf rust, Puccinia hordei. 1. Effect of cultivar and development stage on latent period. Euphytica 24, 21- 27.
Parlevliet, J. E. (1977) Variation in partial resistance in a cultivar of rye, Secale cereale, to brown rust, Puccinia recondita f. sp. recondite. Cereal Rust Bull. 5, 13- 16.
Pretori, Z. A., Singh, R. P. Wagoire, W. and Payne, T. S. (2000) Detection of virulence to wheat stem rust resistance gene Sr31 in puccinia graminis f. sp. tritici in Uganda. Plant Dis. 84, 203.
 Roelfs, A. P., Singh, R. P. and Saari, E. E. (1992) Rust Diseases of Wheat: Concepts and Methods of Diseases Management. D.F. CIMMYT, Mexico, 81 p.
Salvile, D. B. O. (1983) Puccinia striiformis. Fungi. Canadenses 250. Singh, R.P., and Rajaram, S. (1994) Genetics of adult plant resistance to stripe rust in ten spring bread wheat. Euphytica , 72, 1- 7.
Spehar, V. (1966) The appearance of yellow rust in Croatia. Proceeding of the European 122 WheatResistance to Stripe Rust and Mediterranean Cereal Rust Conference, Cambridge, pp. 65- 67.
Stubbs, R.W. (1985) Stripe Rust. The Cereal Rusts, Diseases, Distribution, Epidemiology, and control (eds. A. P. Roelf and W.R. Bushnell). Vol. II. PP 61-101.
 Academic Press. New York Torabi, M., Mardouchi, W, Nazari, K., Golzar, H. and Kashani, A. S. (1995) Effectiveness of wheat Yellow Rust Resistance Gene in Different Part of Iran. Cereal Rust and Powdery Mildew Bulletin 23, 9- 12.
Wellings, C. R. and R. A. McIntosh. (1990) Puccinia striformis f. sp. tritici in Australia: pathogen change during the first 10 years. Plant Pathol. 39, 316- 325.
Wiese, M.V. (1977) Copendium of Wheat Diseases. The American Phytopathogical Society, St. Paul. 106 p.