Effects of Centaurea cineraria extract on the growth of some dermatophytes

Document Type : Research Paper

Authors

Educational Directorate of Karbala, Karbala, Iraq

10.22124/cjes.2022.6040

Abstract

This research was carried out in the Imam Al-Hussein Center, Karbala Governorate, Iraq for Researcher Care and Manuscript Restoration to assess the effects of active ingredient extracted from the Centaurea cineraria methanolic on inhibiting the growth of the cutaneous fungus Trichophyton rubrum and Microsporum canis and determining its appropriate concentration. The plant was chemically tested in the experiment to diagnose active chemicals utilizing qualitative analysis of compounds in the plant sample using the GC-MS technique. Different treatments (concentrations) of 10, 20, 30, 40, 50 and 60% of the methanolic extract were prepared, and the results were obtained as a function of colony diameter on SDA medium along with the colony weight on SD broth medium after 14 days of incubation, and also observing the shape of fungal colonies on the culture medium. The concentrations of the methanolic extract affected the studied fungi, where T60% on Trichophyton exhibited to be the most effective, with a diameter of 9.67 mm, while T10% on Microsporum displayed the lowest effect with a diameter of 77.67 mm. As for the dry weight of the fungal colony, T60% was more effective on Trichophyton, where the average dry weight was 0.106 g, while the T10% on Microsporum exhibited the lowest effect with a dry weight rate of 0.346 g.

Keywords

References

 Abd Elaah, AG & Ahmed, SS 2005, Effect of Fluconazole on Mycelial Growth and Protein Profiles of Some Fungal Species Isolated from Molasses. Assiut University Journal of Botany, 341: 31-45.
Albayrak, S, Atasagun, B & Aksoy, A 2017, Comparison of phenolic components and biological activities of two Centaurea sp. obtained by three extraction techniques. Asian Pacific Journal of Tropical Medicine, 10: 599-606.
Al-Shurait, EA & Al-Ali, RM 2022, Optimal conditions for anthocyanins extracting from some food wastes. Caspian Journal of Environmental Sciences, 20: 503-512.
 Aly, R 1994, Ecology and epidemiology of dermatophyte infections. Journal of the American Academy of Dermatology, 31: S21-S25.
Arey, NC 2010,  Manual of environmental analysis, Ane Books Pvt Ltd, New Delhi, India, 424.
Arnous, A, Dimitris, PM & Kefalas, P 2002, Correlation of pigment and flavanol content with antioxidant properties in selected aged regional wines from Greece. Journal of Food Composition and Analysis, 15: 655-665.
Ayad, R & Akkal, S 2019, Phytochemistry and biological activities of Algerian Centaurea and related genera. Studies in Natural Products Chemistry, Elsevier, 63: 357-414.
Bruno, M, Milia, A, Catinella, G & Bancheva, S 2018, Volatile components from aerial parts of Centaurea diffusa and C. micrantha ssp. melanosticta and their biocidal activity on microorganisms affecting historical art crafts. Natural Product Communications, 13.
Chen, HM, Shiu Chiu, Ch, Jao Chang, L, Chia Ching, Ch, Murugan, M &  Jack, RW 2003, Transmission electron microscopic observations of membrane effects of antibiotic cecropin b on escherichia coli. Microscopy Research and Technique, 62: 423-430.
Steel, RGD & Torrie, GH 1986, Principles and Procedures of Statistics A Biometrical Approach. McGraw-Hill, 813 p.
Drake, LA, Dinehart, SM, Farmer, ER, Goltz, RW, Graham, GF, Hardinsky, MK, Lewis, CW, Pariser, DM, Skouge, JW, Webster, SB, Whitaker, DC, Butler, B, Lowery, BJ, Elewski, BE, Elgart, ML, Jacobs, PH, Lesher, JL & Scher Jr, RK 1996, Guidelines of care for superficial mycotic infections of the skin: Tinea corporis, Tinea cruris, Tinea faciei, Tinea manuum, and Tinea pedis. Journal of the American Academy of Dermatology, 34: 282-286.
Firenzouli, F & Gori, L 2007, Herbal medicine today: clinical and research issues. eCAM, 4:37- 40.
Garcia Jacas, N, Susanna, A, Mozaffarian, V & Ilarslan, R 2000, The natural delimitation of Centaurea (Asteraceae: Cardueae): ITS sequence analysis of the Centaurea jacea group. Plant Systematics and Evolution, 223: 185-199.
Grienke, U, Brkanac, SR, Vujˇci´c, V, Urban, E, Ivankovi´c, S, Stojkovi´c, R, Rollinger, JM, Kralj, J, Brozovic, A & Radi´c Stojkovi´c, M 2018, Biological activity of flavonoids and rare sesquiterpene lactones isolated from Centaurea ragusina L. Frontier Pharmacology 22: 972.
Güven, K, Celik, S & Uysal, I 2005, Antimicrobial activity of Centaurea. species. Pharmaceutical Biology, 43: 67-71.
Guvensen, NC, Keskin, D, Gunes, H, Oktay, MK & Yildirim, H 2019, Antimicrobial property and anti-proliferative activity of Centaurea babylonica (L.) L. on human carcinomas and cervical cancer cell lines. Annals of Agricultural and Environmental Medicine, 26.
Hayes, ML & Berkovitz, BKB 1979, The reduction of fissure caries in Wistar rats by a soluble salt of nonanoic acid. Archives of Oral Biology, 24: 663-666.
Hernández Pérez, M, López García, RE, Rabanal, RM, Darias, V & A. Arias, A 1994, Antimicrobial activity of visnea mocanera leaf extracts. Journal of Ethnopharmacology, 41: 115-119.
Heywood, VH, Moore, D, Richardson, I & Stearn, WT 1978, Flowering plants of the world; Oxford University Press Oxford: Oxford, UK, Volume 336.
 Jones, FA 1996, Herbs useful plants. Their role in history and today. European Journal of Gastroenterology & Hepatology, 8: 1227-1231.
Kady, El & El Maraghy, SS 1993, ntibacterial and antidermatophyte activities of some essential oils from spices. Journal of Sciences, 13: 63-69.
Khanzada، SA, Iqbal, ShM & Akram, A 2006, In vitro efficacy of plant leaf extracts against sclerotium rolfsii sacc. Mycopathologia, 4: 51-53.
Krasnov, EA, Kaminskij, IP, Kadyrova, TV, Pekhen’ko, VG, Adekenov, SM 2012, Antimicrobial activity of extracts from the aerial part Centaurea scabiosa (Asteraceae). Rastitel'nye Resursy, 48: 262-266.
Lee, KW, Young, JK, Hyong, JL & Chang ,YL 2003, Cocoa has more phenolic phytochemicals and a higher antioxidant capacity than teas and red wine. Journal of Agricultural and Food Chemistry, 51: 7292-7295.
Lockowandt, L, Pinela, J, Roriz, CL, Pereira, C, Abreu, RM, Calhelha, RC & Ferreira, IC 2019, Chemical features and bioactivities of cornflower (Centaurea cyanus L.) capitula: The blue flowers and the unexplored non-edible part. Industrial Crops and Products, 128: 496-503.
Magill, SS, Manfredi, L, Swiderski, A, Cohen, B & Merz, WG 2007, Isolation of Trichophyton violaceum and Trichophyton soudanese in Baltimore, Maryland. Journal of Clinical Microbiology, 45: 461-465.
Naeim, H, El Hawiet, A, Rahman, RAA, Hussein, A, El Demellawy, MA & Embaby, AM 2020, Antibacterial activity of Centaurea pumilio L. root and aerial part extracts against some multidrug resistant bacteria. BMC Complementary Medicine and Therapies, 20: 1-13.
Naser AL-Isawi, HI 2022, Effects of applying cold and hot aqueous extracts of ginger to control onion rot disease caused by Aspergillus niger. Caspian Journal of Environmental Sciences, 20: 611-616.
Nowrozi, H, Nazeri, G, Adimi, P, Bashashati, M & Emami, M 2008, Comparison of activities of four antifungal agents in an in vitro model of dermatophyte nail infection. Indian Journal of Dermatology, 53:125-128.
Özcan, K, Acet, T & Çorbacı, C 2019, Centaurea hypoleuca DC: Phenolic content, antimicrobial, antioxidant and enzyme inhibitory activities. South African Journal of Botany, 127: 313-318.
Pohl, CH, Kock, JL & Thibane, VS 2011, Antifungal free fatty acids: a review. Science against microbial pathogens: Communicating Current Research and Technological Advances, 3: 61-71.
Porusia, M, Septiyana, D 2021, Larvicidal activity of Melaleuca leucadendra leaves extract against Aedes aegypti. Caspian Journal of Environmental Sciences, 19: 277-285.
Reed, JD 1995, Nutritional toxicology of tannins and related polyphenols in forage legumes. Journal of Animal Science, 73: 1516-1528.
Reyhan, ARİF, Küpeli, E & Ergun, F 2004, The biological activity of Centaurea L. species. Gazi University Journal of Science, 17: 149-164.
Salih, AH Salih, RY & Ahmed, H 2022, Bioactivity of Cymbopogon Citratus aqueous extract against measles virus and some bacterial isolates. Caspian Journal of Environmental Sciences, 20: 585-592.
Sandve, P & Lassen, J 1999, Importance of selective media for recovery of yeasts from clinical specimens. Journal of Clinical Microbiology, 37: 3731-3732.
Senatore, F, Rigano, D, De Fusco, R & Bruno, M 2003, Volatile components of Centaurea cineraria L. subsp. umbrosa (Lacaita) Pign. and Centaurea napifolia L. (Asteraceae), two species growing wild in Sicily. Flavour and fragrance journal, 18: 248-251.
Sentandreu, R, M. Victoria Elorza, M, Eulogio Valentin, E, Jose Ruiz Herrera, J, San Blas, G & R. Calderone, R 2004, The structure and composition of the fungal cell wall. pathogenic fungi structural biology and taxonomy. G, San-Blas (Ed). Academic Press, Washington DC, pp. 7-37.
Shehata, AS, Mukherjee, PK & Ghannoum, MA 2008, Comparison between the standardized Clinical and Laboratory Standards Institute M38-A2 method and a 2, 3-bis (2-methoxy-4-nitro-5-[(sulphenylamino) carbonyl]-2 H-tetrazolium hydroxide-based method for testing antifungal susceptibility of dermatophytes. Journal of Clinical Microbiology, 46: 3668-3671.
 Soumyanath, A 2005, Traditional medicines for modern times: antidiabetic plants. CRC press, ISBN 9780415334648. 336 p.
Webster، D, Taschereau, P, Belland, RJ, Sand, C & Rennie, RP 2008, Antifungal activity of medicinal plant extracts; preliminary screening studies. Journal of Ethnopharmacology, 115: 140-146.
Yassin, SG & Mohammed, BT 2021, Evaluation of mineral, nano-zinc and fluconazole interaction on some growth characteristics of Trichophyton rubrum and Microsporum canis. Biochemical and Cellular Archives, 21: 1359-1369.
Caspian Journal of Environmental Sciences
Volume 20, Issue 5
Special Issue: Economical Aspects of Environmental Studies
December 2022
Pages 929-937

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