Effects of different levels of dietary zinc supplementation on the testis parameters of the Japanese quail males

Document Type : Research Paper


Department of Animal Production, College of Agriculture, University of Anbar, Ramadi, Iraq


This study was conducted in poultry farm of Agriculture College, University of Anbar, Iraq to assess the effects of different levels of zinc as dietary supplementation in volume density and relative weight of Japanese quail, Coturnix japonica testis contents. Sixty males of 35 day-olds were used in this study. Quails distributed randomly in four treatments and three replicates, each including 5 males. Birds fed balanced diets contain four levels of metal zinc as: T1: control diet without any addition of zinc; T2: diet by adding 25 mg kg-1; T3: 50 mg kg-1; and T4: 75 mg kg-1. Males were slaughtered at 147th day-old then testes weight, testes relative weight, seminiferous tubules, interstitial cells volume density and relative weights (mg g-1) as well as diameters of seminiferous tubules were measured. Results exhibited no significant differences in testis weight and relative weights in all treatments compared to control group. Significant elevation (p < 0.0001) in volume density of spermatogonia was observed in T3 and T4. Significant differences (p < 0.0001) was found in volume density of spermatocytes in all treatments. There was also significant increasing (p < 0.1433) in total sperm contents in T4. Data refers to significant rise (p < 0.0024) in relative weight of spermatogonia in T3, and also significant elevation (p < 0.0453) in volume density of spermatocytes in T3 and T4.


Abdulateef, SM, Majid, AA, Al-Bayer, MA, Shawkat, SS, Tatar, A, Mohammed, TT, Abdulateef, FM, Mohammed & Al Ani, Q  2021, Effect of aromatase inhibitors on sex differentiation and embryonic development in chicks. Veterinary Medicine and Science, 7: 2362-2373.
Al Bayar, MA, Abdulateef, SM, Farhan, SM, Shawkat, SS & Mohammed, ThT 2020, Role of nitroglycerine injection in Japanese Quail (Coturnix japonica) testes tissues parameters. Indian Journal of Ecology, 47: 251-255.
Babaei, H, Derakhshanfar, A, Kheradmand, A & Bazi, J 2007, Zinc modulates heat-induced degenerative effects in mice testes. Iranian Journal of Veterinary Research, 8: 298-303 (In Persian).
Barb, CR, Kraeling, RR, Barrett, JB, Rampacek, GB, Campbell, RM & Mowles, TF 1991, Serum glucose and free fatty acids modulate growth hormone and luteinizing hormone secretion in the pig. Proceedings of the Society for Experimental Biology and Medicine, 198: 636-642.
Chand, N, Naz, S, Khan, A, Khan, S & Khan, RU 2014, Performance traits and immune response of broiler chicks treated with zinc and ascorbic acid supplementation during cyclic heat stress. International Journal of Biometeorology, 58: 2153-2157.
Duncan, DB 1955, Multiple range and multiple F tests. Biometrics, 11: 1-42.‏
Egwurugwu, JN, Ifedi, CU, Uchefuna, RC, Ezeokafor, EN & Alagwu, EA 2013, Effects of zinc on male sex hormones and semen quality in rats. Nigerian Journal of Physiological Sciences, 28: 17-22.
Feng, J, Ma, WQ, Niu, HH, Wu, XM, Wang, Y & Feng, J 2010, Effects of zinc glycine chelate on growthhaematological,and immunological characteristics in broilers. Biological Trace Element Research, 133: 203-211.
Fu, Z, Kubo, T, Noguchi, T, & Kato, H 2001, Developmental changes in the mRNA levels of IGF-I and its related genes in the reproductive organs of Japanese quail (Coturnix coturnix japonica). Growth Hormone & IGF Research, 11: 24-33.
Khoobbakht, Z, Mehr, MRA, Mohammadi, M, Mohammadghasemi, F & Sohani, MM 2020, Supplementation of various zinc sources modify sexual development and testicular IGF family gene expression in pre-pubertal male Japanese quail. Research in veterinary science, 130: 87-92 ‏(In Persian).
Kothari, RP & Chaudhari, AR 2016, Zinc levels in seminal fluid in infertile males and its relation with serum free testosterone. Journal of clinical and diagnostic research: Journal of Clinical and Diagnostic Research, 10: CC05.‏
Kumar, S 2003, Management of infertility due to mineral deficiency in dairy animals. Proceedings of ICAR summer school on “Advance diagnostic techniques and therapeutic approaches to metabolic and deficiency diseases in dairy animals”. Held at IVRI, Izatnagar, UP (15th July to 4th August), 128-137.
Mir Rasekhian, ME, Khara, H & Pourali, HR 2022 Effects of dietary magnesium supplementation on the growth performance, body composition, and immune indices of juvenile Persian sturgeon, Acipenser persicus. Caspian Journal of Environmental Sciences, 20: 557-564.
Pitetti, JL, Calvel, P, Zimmermann, C, Conne, B, Papaioannou, MD, Aubry, F & Nef, S 2013, An essential role for insulin and IGF1 receptors in regulating Sertoli cell proliferation, testis size, and FSH action in mice. Molecular endocrinology, 27: 814-827.
Roitman, R, Schatton, W, Ilyich Maevsky, E 2021, Evaluating the environmental and therapeutic impacts of dietary supplement (Case study: The supplement Oyox for prevention of environment damages, treatment and disorders in the hepatobiliary system). Caspian Journal of Environmental Sciences, 19: 495-502.
Ruiz Cortés, ZT 2012, Gonadal sex steroids: production, action and interactions in mammals. Steroids-From Physiology to Clinical Medicine, pp. 3-44.‏ DOI: 10.5772/52994.
Sahraei, M & Janmohammadi, H 2014, Relative bioavailability of different zinc sources based on tissue zinc concentration in broiler chickens. Iranian Journal of Applied Animal Science, 4: 817-825 (In Persian).
 SAS, Institute 2001, SAS User’s Guide: Statistics Version 6.12 Edition., SAS Institute, Inc., Cary, NC, USA.
Tako, E, Ferket, PR & Uni, Z 2004, Effects of in ovo feeding of carbohydrates and B-hydroxy-B- Methylbutyrate on the development of chicken intestine. Poultry Science, 83: 2023-2028.
Tupe, RS, Tupe, SG, Tarwadi, KV & Agte, VV 2010, Effect of different dietary zinc levels on hepatic antioxidant and micronutrients indices under oxidative stress conditions. Metabolism, 59: 1603-1611.‏
Uni, Z, Ganot, S & Sklan, D 1998, Post-hatch development of mucosal function in the broiler small intestine. Poultry Science, 77: 75-82.‏
Weible, E 1979, Stereological methods. Academic Press, New York,
ISBN: 0127422013.
Yoon, MJ & Roser, JF 2010, Insulin-like growth factor-I (IGF-I) protects cultured equine Leydig cells from undergoing apoptosis. Animal Reproduction Science, 122: 353-358.
Zahmatkesh, A, Karimzadeh, K, Faridnia, M 2020, Effect of dietary selenium nanoparticles and chitosan oligosaccharide on biochemical parameters of Caspian roach (Rutilus caspicus) under malathion stress. Caspian Journal of Environmental Sciences.