Light-emitting bacteria from the Caspian Sea, North of Iran

Document Type : Research Paper

Authors

1 Department of Biology, East Tehran branch, Islamic Azad University, Tehran, Iran

2 Department of Microbiology, Faculty of Sciences, Karaj Branch, Islamic Azad University, Karaj, Iran

Abstract

Bioluminescence is the production and emission of light by living organisms. This phenomenon occurs widely in different organisms. In fact, light-emitting bacteria are the most abundant and widespread luminescent organisms found in marine, freshwater and terrestrial habitats. In this study, the sea water samples were collected from the Caspian Sea, North of Iran. Luminous colonies were observed after an overnight incubation at 25°C on nutrient sea water complete medium. One luminous bacterium isolated, identified and named as Vibrio sp. isolate Caspian based on biochemical tests and 16S rRNA gene sequencing. Besides, two genes involved in bioluminescence, luxA and luxB were sequenced. Light emission measurement was performed for this bacterium  using luminometer, then compared with a control sample previously isolated from water samples in south of Iran. Vibrio sp. Caspian had a weak light emission in comparison with control sample. Sequencing result of 16S rRNA, luxA, and luxB genes showed that these sequences were highly similar to V. vulnificus. However, there were some difference in both nucleotide and amino acid sequences of luciferase. Phylogenetic analysis based on luciferase nucleotide sequences also showed high degree of relationship between the present tested Vibrio and other related species. In general, the present study revealed the capability of indigenous marine sources of Iran for providing bioluminescent bacteria with different characteristics which may result in finding new luminous systems with various applicable capacities

Keywords

References

Baldwin, TO, Christophera, JA, Raushel, FM, Sinclair, JF, Ziegler, MM, Fisher, AJ & Rayment, I, 1995, Structure of bacterial luciferase. Current Opinion in Structural Biology, 5: 798-809.
Baumann, P, Baumann, L, Woolkalis, MJ & Bang, SS 1983, Evolutionary relationships in Vibrio and Photobacterium.A basis for a natural classification. Annual Review of Microbiology, 37: 369-398.
Boettcher, K & Ruby, EG 1990, Depressed light emission by symbiotic Vibrio fischeri of the sepiolid squid Euprymnascolopes. Journal of Bacteriology, 172: 3701-3706.
Budsberg, KJ, Wimpee, CF & Braddock, JF 2003, Isolation and Identification of Photobacterium phosphoreum from an Unexpected Niche: Migrating Salmon. Applied and Environmental Microbiology, 69: 6938–6942.
Chimetto, LA, Cleenwerck, I, Alves, N Jr, Silva, BS, Brocchi, M, Willems A, De Vos, P & Thompson, FL 2011, Vibrio communis sp. nov., isolated from the marine animals Mussismilia hispida, Phyllogorgia dilatata, Palythoa caribaeorum, Palythoa variabilis and Litopenaeus vannamei. International Journal of Systematic Evolutionary Microbiology, 61: 362-368.
Davis, J & Sizemore, R 1982, Incidence of Vibrio species associated with blue crabs (Callinectes sapidus) collected from Galveston Bay, Texas. Applied and Environmental Microbiology, 43: 1092-1097. 
Felsenstein, J 1985, Confidence limits on phylogenies: An approach using the bootstrap. Evolution, 39: 783-791.
Gasteiger, E, Gattiker, A, Hoogland, C, Ivanyi, I, Appel, RD & Bairoch, A 2003, ExPASy: the proteomics server for in-depth protein knowledge and analysis. Nucleic Acids Research, 31: 3784-3788.
Gradoville, MR, Crump, BC, Häse, CC & White AE 2018. Environmental controls of oyster-pathogenic Vibrio spp. in Oregon estuaries and a shellfish hatchery. Applied and Environmental Microbiology, 84: e02156-17.
Stewart, GS & Williams, P 1992, Lux genes and the applications of bacterial bioluminescence. Journal of General Microbiology, 138: 1289-1300.
Haddock, SHD, A Moline, M & James F Case, J 2010, Bioluminescence in the Sea. Annual Reviews of Marine Science, 2: 443-493.
Hastings, JW, Potrikas, CJ, Gupta, SC, Kurfurst, M & Makemson, JC 1985̗ Biochemistry and physiology of bioluminescent bacteria. Advances in Microbiology Physiolgy, 26: 235-291.
Herring, P 2002, Marine microlights: the luminous marine bacteria. Microbiology Today, 29: 174-176.
Horseman, MA & Surani, SA 2011, comprehensive review of Vibrio vulnificus: an important cause of severe sepsis and skin and soft-tissue infection. International Journal of Infectious Diseases, 15: e157-166.
Jabalameli, L, Razavi, MR, Hoseinkhani, S & Akhavan Sepahi, A 2015, Isolation, identification and characterization of new luminous bacteria from Chah Bahar Port, southern marine habitat of Iran. Iranian Journal of Fisheries Sciences, 14: 555-566.
Kimura, MA 1980, simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences. Journal of Molecular Evolution, 16: 111-120.
Kita-Tsukamoto, K, Wada, M, Yao, K, Kamiya, A, Yoshizawa, S, Uchiyama, N & Kogure, K 2006, Rapid identifcation of marine bioluminescent bacteria by amplifed16S ribosomal RNA gene restriction analysis. FEMS Microbiology Letters, 256: 298–303.
Matheson, IB, Lee, J & Müller, F 1981, Bacterial bioluminescence: Spectral study of the emitters in the in vitro reaction. Proceedings of the National Academy of Sciences of the United States of America, 78: 948-952. 
McCann, J, Stabb, EV, Millikan, DS & Ruby, EG 2003, Population dynamics of Vibrio fischeri during infection of Euprymna scolopes. Applied and Environmental Microbiology, 69: 5928–5934.
Meighen, EA 1988, Enzymes and genes from the lux operons of bioluminescent bacteria. Annual Review of Microbiology, 42: 151-176.
Meighen, EA 1994, Genetics of bacterial bioluminescence. Annual Review of Genetics, 28: 117-139.
Meighen, EA 1991, Molecular biology of bacterial bioluminescence. Microbiology Reviews, 55: 123–142.
Mohseni, M, Abbaszadeh, J, Maghool, Sh-S & Chaichib, MJ 2018, Heavy metals detection using biosensor cells of a novel marine luminescent bacterium Vibrio sp. MM1 isolated from the Caspian Sea. Ecotoxicology and Environmental Safety, 148: 555-560.
NCBI Resource Coordinators 2017, Database resources of the National Center for Biotechnology Information. Nucleic Acids Research, 45: D12-D17.
O’Grady, EA & Wimpee, CF 2008, Mutations in the lux operon of natural dark mutants in the genus Vibrio. Applied and Environmental Microbiology, 74: 61–66.
Oliver, JD, Roberts, DM, White, VK, Dry, MA & Simpson, LM 1986,  Bioluminescence in a strain of human pathogenic bacterium Vibrio vulnificus, Applied and Environmental Microbiology, 52: 1209-1211.
Omeroglu, EE & Karaboz, I 2012, Characterization and genotyping by pulsed-field gel electrophoresis (PFGE) of the first bioluminescent Vibrio gigantis strains. African Journal of Microbiology Research, 6: 7111-7122.
Peat, SM & Adams, BJ 2008, Natural selection on the luxA gene of bioluminescent bacteria. Symbiosis, 46: 101–108.
Phillips, KE & Satchell, KJF 2017, Vibrio vulnificus: from oyster colonist to human pathogen. PLOS Pathogens. 13: e1006053. 
Tamura, K, Stecher, G, Peterson, D, Filipski, A, Kumar, S 2013, Molecular evolutionary genetics analysis version 6.0. Molecular Biology and Evolution, 30: 2725-2729.
Tanet, L, Tamburini, C, Baumas, C, Garel, M, Simon, G, Casalot, L, 2019, Bacterial Bioluminescence: Light Emission in Photobacterium phosphoreum Is Not Under Quorum-Sensing Control. Frontiers in Microbiology, 10: 365.
Tsai, I 2018, Tracing speciation of Vibrio bacteria in the Harveyi clade. Biodiversity Research Center, Academia Sinica, 128 Academia Road, Section 2, Nankang, Taipei 11529, Taiwan. 1p.
Urbanczyk, H, Ast, JC, Kaeding, A.J, Oliver, JD, & Dunlap, PV 2008, Phylogenetic analysis of the incidence of lux gene horizontal transfer in Vibrionaceae. Journal of Bacteriology, 190: 3494-3504.
Yaser, NA, Abdullah, MFF, Aris, AM & Zainudin, II 2014, Isolation and identification of bioluminescent bacteria in squid and water of Malaysia. International Journal of Advances in Agricultural and Environmental Engineering, 1: 225-228.
Caspian Journal of Environmental Sciences
Volume 17, Issue 4
December 2019
Pages 295-304

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