Determination of polycyclic aromatic hydrocarbons (PAHs) in water, sediment and tissue of five sturgeon species in the southern Caspian Sea coastal regions


1 O. Rafieyan*1, A. A. Darvishsefat2, S. Babaii1, A. Mataji1

2 H.A. Khoshbavar-Rostami1


Concentration levels of 16 polycyclic aromatic hydrocarbons (PAHs) consisting of naphthalene, acenaphthylene, acenaphthene, fluorine, phenantherene, anthracene, fluor?anthene, pyrene, benzo(a)anthracene, chrysene, benzo(b)fluoranthene, benzo(k)fluoranthene, benzo(a)p?yrene, dibenzo(a,h)ant?hracene, indeno(1,2,3-cd) pyrene and benzo(g,h,i)perylene were measured in water and sediment samples collected from the southern Caspian Sea coasts at four stations of Noshahr, Freydoonkenar, Sari and Amirabad during autumn, winter and spring of 2005-2006. Also, tissue samples from five sturgeon species including stellate sturgeon, Persian sturgeon, beluga, Russian sturgeon and ship sturgeon were obtained. Samples were analyzed by gas chromatography after the extraction process and the obtained data were statistically analyzed using one-way ANNOVA analysis at confidence level 95%. The mean concentrations of PAHs in water and sediment samples were in the range of 0.004-2.946 mgl-1 and 0.024-2.336 ?g g-1, respectively. No significant difference was seen in the mean concentrations of PAHs among stations and seasons of the examined samples. Also, mean concentration of PAHs in the liver, muscle, gill, kidney and gonad five of sturgeon species was 0.81-1.34 ?gg-1. The results of this study show that the levels of PAHs in water, sediment and sturgeon organs were below the acceptable levels of PAHs for the south Caspian Sea coastal regions.


Aas, E., Beyer, J., Jonsson, G., Reichert, W.L. and Andersen, O.K (2001) Evidence of uptake, biotransformation and DNA binding of polyaromatic hydrocarbons in Atlantic cod and corkwing wrasse caught in the vicinity of an aluminum works. Marine Enviromental Research.52: 213–229.
Agusa, T., Kunito, T., Tanabe, S., Pourkazemi, M. and Aubrey, D. G (2004) Concentrations of trace elements in muscle of sturgeons in the Caspian Sea. Marine Pollution Bulletin. 49: 789–800.
American Society for Testing and Material (ASTM) (1995) D4281, pp.4-41.Anderson, J.W. and Lee, R.F (2006) Use of biomarkers in oil spill risk assessment in the marine environment. Human andEcological Risk Assessment. 12:1192–1222.
Beyer, J., Jonsson, G., Porte, C., Krahn, M. M. and Ariese, F (2010) Analytical methods for determining metabolites of polycyclic aromatic hydrocarbon (PAH) pollutants in fish bile: A review. Environmental Toxicology and Pharmacology. 30: 224–244.
Billard, R. and Lecointre, G (2001) Biology and conservation of sturgeon and paddlefish. Review of Fish Biology and Fisheries. 10:355–392.
Buryskova, B., Hilscherova, K., Blaha, L., Marsalek, B. and Holoubek, I (2006)
Khoshbavar-Rostamiet al.,141Toxicity and modulations of biomarkers in Xenopus laevis embryos exposed to polycyclic aromatic hydrocarbons and their N-heterocyclicderivatives. Environm-entalToxicology.21:590–598.
Colombo, J.C., Barreda, A., Bilos, C., Cappeleetti, N., Migoya, M.C. and Skorupka, C (2005) Oil spill in the Rio de la Plata estuary, Argentina: 2-hydrocarbon disappearance rate in sediments and soils. EnvironmentalPollution. 134: 267-276.
Dahl, C. and Kuralbayeva, K.E (2001) Energy and the environment in Kazakhstan. Energy Policy.29: 429–440.
De Mora, S., Villeneuve, J-P., Sheikholeslami, M. R., Cattini, C. and Tolosa, I (2004) Organochlorinated compounds in Caspian Sea sediments. MarinePollutionBulletin.48: 30–43.
Deb, S. C., Araki T. and Fukushima, T (2000) Polycyclic Aromatic Hydrocarbons in Fish Organs.MarinePollutionBulletin. 40: 882-885.
Effimoff, I (2000) the oil and gas resource base of the Caspian region. Journal of Petroleum Science and Engineering. 28:157–159.
Espinoza-Quin ̃ones, F.R., Mo ́ denes, A.N., Pala ́ cio, S.M., Szymanski , N., Welter , R.A., Rizzutto , M.A., Borba, C.E. and Kroumov , A.D (2010) Evaluation of trace element levels in muscles, liver and gonad of fish species from São Francisco River of the Parana ́ Brazilian state by using SR-TXRF technique. Journal Applied Radiation and Isotopes.68: 2202–2207.
Figueiredo-Fernandes, A., Ferreira-Cardoso, J.V., Garcia-Santos, S., Monteiro, S.M., Carrola, J., Matos, P. and Fontainhas-Fernandes, A (2007) Histopathological changes in liver and gill epithelium of Nile tilapia, Oreochromis niloticus, exposed to waterborne copper. PESQUISA VETERINÁRIA BRASILEIRA.Brazil-ian Journal of Veterinary Research. 27:103–109.
Habibi, M. H. and Hadjmohammadi, M. R (2008) Determination of Some Polycyclic Aromatic Hydrocarbons in the Caspian Seawater by HPLC Following Preconcentration with Solid-Phase Extraction. Iranian Journal of Chemistry and Chemical Engineering.27: 91-96.
Hosseini, S.V., Dahmardeh Behrooz, R., Esmaili-Sari, A., Bahramifar, N., Hosseini, S. M., Tahergorabi, R., Hosseini, S.F. and Feás , X (2008) Contamination by organochlorine compounds in the edible tissue of four sturgeon species from the Caspian Sea (Iran). Chemosphere. 73: 972–979.
Jarić, I., Višnjić-Jeftić, Ž., Cvijanović, G., Gačić, Z., Jovanović, L., Skorić, S. and Lenhardt , M (2011) Determination of differential heavy metal and trace element accumulation in liver,gills, intestine and muscle of starlet (Acipenser ruthenus) from the Danube River in Serbia by ICP-OES. Microchemical Journal. 98: 77–81.
Kajiwara, N., Ueno, D., Monirith, I., Tanabe, S., Pourkazemi, M. and Aubrey, D. G (2003) Contamination by organochlorine compounds in sturgeons from Caspian Sea during 2001 and 2002. MarinePollutionBulletin.46: 741–747.
Kannan, K. and Perrotta, E (2008) Polycyclic aromatic hydrocarbons (PAHs) in livers of California sea otters. Chemosphere.71: 649–655.
Khodorevskaya, R.P., Zhuravleva, O.L. and Vlasenko, A.D (1997) Present status ofcommercial stocks of sturgeons in the Caspian Sea basin. Environmental Biology of Fishes. 48:209–219.
Korotenkoa, K.A., Mamedovc, R.M., Kontarb, A.E. and Korotenko, L.A (2004) Particle tracking method in the approach for prediction of oil slick transport in the sea: modelling oil pollution resulting from river input, Journal of Marine Systems. 48: 159–170.
Kurunthachalam, K. and Perrotta, E (2008) Polycyclic aromatic hydrocarbons (PAHs) in livers of California sea otters. Chemosphere. 71: 649–655.
Lee, R.F. and Anderson, J.W (2005) Significance of cytochrome P450 system responses and levels of bile fluorescent aromatic compounds in marine wildlife following oil spills. MarinePollutionBulletin. 50:705–723.
Mishra, A.K. and Mohanty, B (2008) Acutetoxicity impacts of hexavalent chromium on behavior and Determination of polycyclic aromatic hydrocarbons...142histopathology of gill, kidney and liver of the freshwater fish,Channa punctatus (Bloch). Environmental Toxicology and Pharmacology. 26: 136–141.
Mohammadi Zadeh, C., Saify, A. and Shalikar, H (2010) Polycylic Aromatic Hydrocarbons (PAHs) along the Eastern Caspian Sea Coast, Mazandaran State, Iran. World AppliedScienceJournal. 9(2): 211-215.
Monteiro, P.R.R., Reis-Henriques, M.A. and Coimbra, J (2000) Plasma steroid levels in female flounder (Platichthys flesus) after chronic dietary exposure to single polycyclic aromatic hydrocarbons. MarineEnvironmentalResearch. 49: 453-467.
MOOPAM.(1999).Manual of ceanographic observations and pollutants analysis methods (Third Edition). The Regional Organisation for the Protection of the Marine Environment (ROPME),
Kuwait.Moore, M. J., Mitrofanov, I. V., Valentini, S. S., Volkov, V. V., Kurbskiy, A. V., Zhimbey, E. N., Eglinton, L. B. and Stegeman, J. J (2003) Cytochrome P4501A expression, chemical contaminants and histopathology in roach, goby and sturgeon and chemical contaminants in sediments from the Caspian Sea, Lake Balkhash and the Ily River Delta, Kazakhstan. MarinePollutionBulletin. 46: 107–119.
Narbonne, J.F., Djomo, J.E., Ribera, D., Ferrier, V. and Garrigues, P (1999) Accumulationkinetics of polycyclic aromatic hydrocarbons adsorbed to sediment by the mollusk Corbicula fluminea. Ecotoxicology and Environmental Safety. 42:1–8.
Nasrollahzadeh, H. and Malekshomali, M. (2000) fluctuation of oil pollution in some important ports of the southern of Caspian Sea, 10th International Congress, Ksapnirkh, Astarkhan, Russia224-25 December.
Nicolas, J-M (1999)Review Vitellogenesis in fish and the effects of polycyclic aromatic hydrocarbon contaminants. Aquatic Toxicology. 45: 77–90.
Oliva, M., Gonza ́ lez deCanales, M.L., Gravato, C., Guilhermino, L. and Perales, J. A (2010) Biochemical effects and polycyclic aromatic hydrocarbons (PAHs) in Senegal sole (Solae senegalensis) from a Huelva estuary (Spain).Ecotoxicology and Environmental Safety. 73: 1842–1851.
Oliveira Ribeiro, C.A., Vollaire, Y., Sanchez-Chardi, A. and Roche, H (2005) Bioaccumulation and the effects of organochlorine pesticides, PAH and heavy metals in the Eel (Anguilla Anguilla) at the CamargueNature Reserve, France. Aquatic Toxicology. 74 : 53–69
Olojo, E.A.A., Olurin, K.B., Mbaka, G. and Oluwemimo, A.D (2005) Histopathology of the gill and liver tissues of the African catfish, Clarias gariepinus exposed to lead. African Journal of Biotechnology.4: 117–122.
Ortiz, J.B., De Canales, M.L.G. and Sarasquete, C (2003) Histopathological changes induced by lindane (γ-HCH) in various organs of fishes. ScientiaMarina. 67: 53–61.
Pak, A. and Farajzadeh, M (2007) Iran’s Integrated Coastal Management plan: Persian Gulf, Oman Sea, and southern Caspian Sea coastlines, Ocean & Coastal Management.50: 754–773.
Pulsford, A.L., Thomas, M.E., Lemaire-Gony, S., Coles, J., Fossato, V.U. and Pipe, R.K. (1995) Studies on the Immune System of the Goby, Zosterisessor ophiocephalus, from the Venice Lagoon. MarinePollutionBulletin.30:586-591.
Reynaud, S. and Deschaux,P (2006) The effects of polycyclic aromatic hydrocarbons on the immune system of fish: A review,Aquatic Toxicology. 77: 229–238.
Salazar-Coria, L., Amezcua-Allieri, M.A., Tenorio-Torres, M. and Gonza ́ lez-Macı ́as, C (2007) Polyaromatic hydrocarbons (PAHs) and metal evaluation after a diesel spill in Oaxaca,Mexico.Bulletin ofEnvironmentalContaminationandToxicology.79:462–467.
Silvestre, F., Linares-Casenave, J., Doroshov, S. I. and Kültz, D (2010) A proteomic analysis of green and white sturgeon larvae exposed to heat stress and selenium. Science of the Total Environment. 408:3176–3188.
Tintos, A., Gesto, M., Alvarez, R., Míguez, J. M. and Soengas, J. L (2006)
Khoshbavar-Rostamiet al.,143Interactive effects of naphthalene treatment and the onset of vitellogenesis on energy metabolism in liver and gonad, and plasma steroid hormones of rainbow trout Oncorhynchus mykiss. Comparative Biochemistry and Physiology. 144: 155–165.
Tolosa, I., de Mora, S. J., Fowler, S. W., Villeneuve, J-P., Bartocci, J. and Cattini, C (2005) Aliphatic and aromatic hydrocarbons in marine biota and coastal sediments from the Gulf and the Gulf of Oman. MarinePollutionBulletin.50: 1619–1633.
Tolosa, I., De Mora, S., Sheikholeslami, M.R., Villeneuve, J.P., Bartocci, J. and Cattini, C (2004) Aliphatic and aromatic hydrocarbons in coastal Caspian Sea sediments. MarinePollutionBulletin. 48 : 44–60.
van der Oost, R., Beyer, J. and Vermeulen, N.P.E (2003) Fish bioaccumulation and biomarkers in environmental risk assessment: a review. Environmental Toxicology and Pharmacology. 13: 57–149.
Wurl, O. and Obbard, J.P (2004) A review of pollutants in the sea-surface microlayer (SML): a unique habitat for marine organisms. MarinePollutionBulletin. 48:1016–1030.
Zheng, R., Wang, C., Zhao, Zuo, Z. and Chen, Y (2005) Effect of tributyltin, benzo(a)pyrene and their mixture exposure on the sex hormone levels in gonads of cuvier (Sebastiscus marmoratus).Environmental Toxicology and Pharmacology. 20:361–367.