The independent effects of ferrous and phosphorus on growth and development of Tetraselmis suecica; an in vitro study


1 Fisheries Department, Islamic Azad University, Lahijan Branch, Lahijan , Iran.

2 Fisheries Department, School of Natural Resources and Marine Science, Tarbiat Modarres University, Noor, Iran.

3 Fisheries Department, Faculty of Natural Resources, University of Guilan, Someh Sara, Guilan, Iran. *Corresponding author's E-mail:


Five treatments including Conway medium, media containing 0.1, 0.17, 0.3 and 0.5 mg l-1 ferrous (Fe; in the first experiment), media with 1, 1.26, 1.59, 2 mg l-1 concentrations of phosphorous (P; in the second experiment) and a pure sample of Tetraselmis suecica were cultured under laboratory conditions. Growth rate of the algae was determined using the optical density method at 750 nm and number of algal cells were counted with a hemocytometer. The results of the first experiment showed that the amount of Fe for maximum growth of this species was 0.3 mg l-1, while Fe concentration in Conway medium was 0.27 mg l-1 (P>0.05). The results of second experiment showed that 1.59 mg l-1 P caused the maximum growth of algae which was not significantly different from that of the control medium (with 1.6 mg l-1; Conway; P>0.05). These results showed that due to the lack of significant differences in maximum growth of this alga recorded in 0.3 mg l-1 Fe and that recorded in 1.59 mg l-1 P in Conway medium, increase or decrease of these doses will have a significant negative effect on algal growth.
AQUACOP. (1984) Aquaculture en milieu tropical. IFREMER Service documentation Publication. B. P. 337- 29273 Brest, Cedex, 469 p.
Brown, M.R. (2002) Nutritional value of microalgae for aquaculture. Advances en nutrition acuicola VI. Memorias del VI. (eds. Cruz-Suares, L. E., D. RicqueMarie, M. Tapia-Salazar, M.G. Gaxiolacortes, and N. Simoes). Simposium internacional de nutrition acuicola. 3-6 Septiemper del 2002. Cancun, Quintanaroo, Mexico.
Borowitzka, M.A. (1999) Commercial production of microalgae: ponds, tanks, tubes and fermenters. J Biotechnol 70, 313-321.
Burlew, J.S. (1953) Algae culture. from laboratory to pilot plant. Carnegie Institution of Washington, Washington, DC.
Fulks, W. and Main, K.L. (1991) The design and operation of commercial-scale live feeds production systems. In: Fulks, W., Main, K.L. (Eds.), Rotifer and Microalgae Culture Systems. The Oceanic Institute, Honolulu, HI, pp. 3-52.
Halama, D. (1990) Single cell protein. Non conventional feed stuffs in the nutrition of farm animals. (ed. Kolma, B). pp. 34-49. Elsevier Science Publishing Company, Inc. New York.
Hansen, P.J. (2000) Use of a hemocytometer. Department of animal science, University of Florida. Pp. 10-15.
Jeffery, S.W., LeRoi, J.M. and Brown, M.R. (1992) Characteristics microalgal species for Australian mariculture. (Eds. Allen, G.L and Dall, W). Proceeding of the National Aquaculture Workshops, April 1991, pp 164-173.
Kawamura, T., Roberts, R.D. and Nicholson, C.M. (1988) Factors affecting the food value of diatom strains for post-larval abalone, Haliotis iris. Aquaculture 160, 81- 88.
Laing, I. and Verdugo, C.G. (1991) Nutritional value of spraydried Tetraselmis suecica for juvenile bivalves. Aquaculture 92, 207-218.
Lavens, P. and Sorgeloos, P. (1996) Manual on the production and use of live food for aquaculture. FAO Technical Paper Publishers, 7-28.
Phany, S.M. (1992) Role of algae in live stock fish integrated farming system. (Eds. Mukherjee, T.K., Moi, P.S., Pandam and Yang, Y.S). Proceeding of the FAO/IPT workshop on integrated live stock fish production system, 16-20 December 1991. University of Malaya, Kuala Lumpur, Malaysia, pp 49-56.
Piri, Z.M. and Ordog, V. (1997) Effect of some herbicides commonly used in Iranian agriculture on aquatic food chain. PhD thesis to Hungarian Academy of Science, pp. 19-30.
Raymont, J.E.C. (1980) Plankton and productivity in the oceans. Vol 1, phytoplankton. Robert, W.H. and James, R. (1979) Methods for microscopic algae. (Ed Stein, R.J). Handbook of phycological methods, culture methods and growth measurements. Cambridge University Press, 447 p.
Soeder, C.J. (1981) Productivity of microalgal systems. U.O.F.S. Publ., Series C 3, 9-15. Stanley, B. (1987) Toxicology of metals. John Wiley and Sons, pp 90-95. Stryer, L. (1988) Photosynthesis. (Ed. Freeman, W.H.). Biochemistry. New York, pp. 285-302.
Sym, S.D. and Pienaar, R.N. (1993) The class prasinophyceae. (Eds. Rund, F.E. and Chapman, D). Progress in phycological research. Biopress Ltd., Bristol, pp. 281-376.