First record of the bacterium Pseudomonas putida on pepper in Iraq


1 Department of Plant Protection, College of Agriculture and Forestry, University of Mosul, Iraq

2 Department of Biology, College of Science, University of Mosul, Iraq

3 Department of Food Sciences, College of Agriculture and Forestry, University of Mosul, Iraq



Random samples were collected from the field soil and pepper fruits in different agricultural areas in order to isolate and identify the associated Pseudomonas putida (Trevisan 1889) Migula 1895. The diagnosis process was carried out based on the culture, phenotypic and biochemical characteristics. Once isolation on the nutrient agar food media, pure single or double colonies of P. putida were appeared, exhibiting the ability to grow in Maconkey medium, negative for gram dye, capable of producing yellowish green Pyouverdin dye under ultraviolet rays, characterizing by an undesirable odor. The molecular diagnosis of bacteria was confirmed by DNA secquencing analysis of the complementary DNA (cDNA) of the double-stranded dsRNA (dsRNA) RNA that forms the genome of bacteria during replication. The extraction was performed using the dsRNA dsRNA extraction method from the studied samples and the results of the sequence analysis were compared with the nucleotide database of the NCBI using BLAST. The isolates of bacteria with similar nucleotide sequences were identified and the Iraqi isolate was placed in the phylogenetic tree diagram to show the relationship between the Iraqi isolate and the global ones. Bacterial isolates were recorded in the National Center for Genetic Bank NCBI, European ENA and the Japanese Information Bank, DNA with Accession Number (s) SUB9666355 AHM MZ209185, which is the first record of P. putida on pepper in Iraq.


Abd, YM, Abdel Reda, HA & Hadwan, HA 2016, The effect of biofertilizer produced from local isolates of Pseudomonas putida and P. flourescens bacteria on some soil characteristics and wheat yield (Tritican aestivam). Iraqi Journal of Agricultural Sciences, 47:1413 -1422
Abbas Zadeh, P, Saleh Rastin, N, Asadi Rahmani, H, Khavazi, K, oltani, A, AShoary Nejati, AR & Miransarim, M 2010, plant growth promoting activities of fluorescent pseudomonads, isolated from the Iranian soils. Acta Phsiologiae Plantarum, 32: 281-288
Alexander, M 1988, Introduction to soil microbiology. Johan Wiley and Sons Inc., New York.
Benizri, E, Schoeny, A, Picard, C, Courtade, A & Gukert A, 1997, External and internal root colonization of maize by two Pseudomonas strains: Enumeration by enzyme linked immuno-sorbent assay. Current Microbiology, 34: 297-302.
Compeau, G, Al Achi, BJ, Platsouka, E & Levy, SB 1988, Survival of rifampicin-resistant mutants of Pseudomonas fluorescens and Pseudomonas putida in soil systems. Applied and Environmental Microbiology, 84: 2432-2438.
Duijff, BJ, Gianinazzi Pearsonand, V & Lemanceau, P 1997, Involvement of the outer membrane Lipoplysaccharides in the endophytic colonization of tomato roots by biocontrol Pseudomonas fluorescens strain WCS417r. New Phytologist, 135: 325-334.
Duque, E, MarqueÂ, S & Ramos, JL 1993, Mineralization of p- methyl-14C-benzoate in soils by Pseudomonas putida (pWW0). Microbial Releases, 2: 175-177.
Franklin, FCH, Bagdasarian, M, Bagdasarian, MM & Timmis, KN1981, Molecular and functional analysis of the TOL plasmid pWW0 from Pseudomonas putida and cloning of genes for the entire regulated aromatic ring meta-cleavage pathway. Proceedings of the National Academy of Sciences USA, 78: 7458-7462.
Goszcynska, T, Serfontein, JJ & Serfontein, S 2000, Introduction of practical phytobacteriology a manual for phytobacteriology, Safrinnet –Loop of BioNet – International, 83 p.
Holt, JG, Kreig, MR, Sneath, PH, Staley, JT & Williams, ST 1994, Bergey’s manual of determinative bacteriology. 9th Edition. Williams and Wilkins, USA, pp. 93-94.
Mills, AAS, Platte, HW & Hurt, RAR2006, Sensitivity of Erwinia spp. from salt compounds in vitro and their effect on the soft rot in potato tubers in storage. Past Harvest Biology, 41: 208-214.
Mohareb, F, Iriondo, M, Doulgeraki, AI, Hoek, VA, Aerks, H, Cauchi, M & Nychas, GL 2015, Identification of meat spoilage gene biomarkers in Pseudomonas putida using gene profiling, Food, 51:152-160           
Palleroni, NJ 1992, Human- and animal-  pathogenic Pseudomonas pp. 3086-3103, In A, Balows, HG, Triiper, M, Dworkin, W, Harder and KH, Schleifer (Eds.), The prokaryotes by handbook on the biology of bacteria: ecophysiology, isolation, identification, applications, vol.3. Springer-Verlag, New York.
Palleroni, NJ 1984, Gram-negative aerobic rods and cocci, family I: Pseudomonadaceae. In: NR, Krieg & JG Holt (Eds.). Bergeys manual of systematic bacteriology. Vol. 1, Williams and Wilkins, Baltimore, 141-199.
Papadopoulou, OS, Iliopoulos, V, Mallouchos, A, Pahagou, EX, Nikos, C, Tassou, C & Nychas, GJ 2020, Spoilage potential of Pseudomonas (P. fragi and P. putida) and LAB (Leuconastoc mesenteroides and Lactobacillus sakei) storage of sterile pork meat using GC/MS and Data Analytics, Journal of Food, 9: 633.
Premono, MAM & Vleck, PLG 1996, Effect of phosphate solubilizing pseudomonas putida on the growth of maize and its survival in the rhizosphere. Indonesian Journal of Crop Sciences, 11:13-23.
Ramos, JL, Duque, E & Ramos Gonza lez MI 1991, Survival in agricultural soils of an herbicide-resistant Pseudomonas putida bearing a recombinant TOL plasmid. Applied and Environmental Microbiology, 57: 260-261.
Rezzonico, F, Zala, M, Keel, C, Duffy, B, Moenne Locccoz, Y & Defago, G 2007, Is the ability of biocontrol fluorescent pseudomonads to produce the antifungal metabolite 2,4-diacetylphloroglucinol really synonymous with higher plant protection. New Phytologist, 173: 861-872.
Schaad, NW 1980, Laboratory guide for identification of plant pathogenic bacteria. American Phyto-pathological Society, Manual, Bacteria rhizosphere competent. Environmental Microbiology, 1: 9-13.
Segura, A, Rodriguez Conde, S, Ramos, C & Romas, JL 2009, Bacterial response and interactions with plant during rhizo-remediation. Microbial Biotechnology, 2: 452-464.
Segura, A & Ramos, JL 2012, Plant–bacteria interactions in the removal of pollutants. Current Opinion in Biotechnology, 24: 1-7.
Stanier, RY, Palleronai, NJ & Doudoroff, M 1966, The aerobic Pseudomonas: A taxonomic study. The Journal of General Microbiology, 43: 159-271
Weimer, A, Kohlstedt, M, Volke, DC, Nikel, PI & Wittmann, C 2020, Industrial biotechnology of Pseudomonas putida advances and prospects. Applied Microbiology and Biotechnology, 104: 7745-7766.
Williams, PA & Murray, K 1974, Metabolism of benzoate and the methylbenzoates by Pseudomonas putida (arvilla) mt-2: Evidence for the existence of a TOL plasmid. Journal of Bacteriology, 120: 416-423.