Possible effects of glucosamine on gait assessment and lipid peroxidation in ovariectomized rabbits

Document Type : Research Paper

Authors

1 People Friendship University of Russia (RUDN University) Moscow, Miklukho-Malaya street 7, Russia

2 Department of Anaesthesia Techniques, Al-Noor University College, Nineveh, Iraq

3 Medical Technical College, Al-Farahidi University, Iraq

4 Medical Laboratory Techniques Department, Al-Mustaqbal University College, 51001 Hillah, Babylon, Iraq

5 Medical Laboratory Techniques Department, Mazaya University College, Iraq

6 National University of Science and Technology, Dhi Qar, Iraq

7 Al-Hadi University College, Baghdad,10011, Iraq

8 Department of Pharmacy, Al-Zahrawi University College, Karbala, Iraq

9 Department of Biological Sciences, Institute of Natural Sciences and Geography, Abai Kazakh National Pedagogical University, Almaty, Kazakhstan

10.22124/cjes.2023.6534

Abstract

In routine physiological procedures and/or pathological conditions, the living tissues produce free radicals, especially reactive oxygen species (ROS). It has been well documented that the primarily responsible mechanism for cell damage and destruction would be the lipid peroxidation conducted by ROS. It is approved that human articular chondrocytes actively lead to significant ROS production. DNA damage and telomere shortening have been known as the most important consequences of any increase in ROS production. Therefore, the current research was designed to investigate the effect of pre- and post- osteoarthritis administration of glucosamine on induced osteoarthritis in rabbits. In this experiment, 20 adult female New Zealand white rabbits with an average weight of 2.14 ± 0.45 kg were used. Visual inspection evaluated the animals to be free of any joints and muscular disorders. The animals were randomly divided into 4 groups (n = 5); Group A was ovariectomized without any treatments, while rabbits in Group B were ovariectomized, and after radiographic confirmation of OA, they were administered 75 mg kg-1 glucosamine. Rabbits in Group C were neither ovariectomized nor administered glucosamine, while rabbits in Group D were ovariectomized and administered glucosamine immediately after ovariectomy. Rabbits' gaits were scored before ovariectomy and at 4, 8, 12, 18, and 24 weeks post-ovariectomy (PO). Stifle joints radiographs and blood samples were obtained at 12, 18, and 24 weeks PO. Gait assessment score (GAS) was significantly (p < 0.05) lower in group A than in groups B, C, and D. Plasma concentration of TBARS was higher in groups A and B than in groups C and D, while plasma SOD decreased significantly (p < 0.05) between weeks 4 and 12 PO in all the groups. However, there were no significant differences in plasma concentrations of GSH, GPx, GST, tCHOL, TRIG, HDL, and LDL in all the groups. It was concluded that glucosamine inhibits lipid peroxidation; its prophylactic use has no significant advantage over its post-OA use.
 

Keywords

References

Abella, V, Scotece, M, Conde, J, López, V, Lazzaro, V & Pino, J et al. 2014, Adipokines, metabolic syndrome and rheumatic diseases, DOI: 10.1155/2014/343746.
Apak, Ra, Özyürek, M & Güçlü, K 2016, Çapanoğlu EJJoa, chemistry f. Antioxidant activity/capacity measurement. 1. Classification, physicochemical principles, mechanisms, and electron transfer (ET)-based assays. 64: 997-1027.
Bruyère, O, Altman, RD & Reginster, JY 2022, Efficacy and safety of glucosamine sulfate in the management of osteoarthritis: Evidence from real-life setting trials and surveys. Seminars in arthritis and rheumatism. 45: S12-7, DOI: 10.1016/j.semarthrit.2015.11.011.
Carlson, AK, Rawle, RA, Wallace, CW, Brooks, EG, Adams, E, Greenwood, MC et al. 2019, Characterization of synovial fluid metabolomic phenotypes of cartilage morphological changes associated with osteoarthritis. 27: 1174-1184.
Cason, RA 2014, Effects of osteoarthritis and chronic pain management for companion animals. 44 p., https://opensiuc.lib.siu.edu/cgi/viewcontent.cgi?article=1671&context=gs_rp.
Chen, AF, Davies, CM, De Lin, M & Fermor, B 2008, Oxidative DNA damage in osteoarthritic porcine articular cartilage. Journal of Cellular Physiology. 217: 828-833, DOI: 10.1002/jcp.21562.
Dadashpour Davachi, N, Masoudi, R, Bartlewski, PM & Ahmadi, B 2022, Didarkhah MJIJoVM. Induction of Ovulation after Artificial Insemination in Rabbits: Intramuscular Injection of Gonadotropin-Releasing Hormone (GnRH) Agonist vs. Intravenous Administration of Mated Doe Serum. 16: 26-35.
Durairajanayagam, D 2018, Lifestyle causes of male infertility. Arab Journal of Urology. 16:10-20. DOI: 10.1016/j.aju.2017.12.004.
Fenton, J, Chlebek Brown, K & Caron, J 2002, Orth MJEVJ. Effect of glucosamine on interleukin‐1‐conditioned articular cartilage. 34: 219-223, DOI: 10.1111/j.2042-3306.2002.tb05422.x.
Gbotolorun, SC, Salako, AA & Ogunlade B 2022, Tiger nut: Antidote for alcohol-induced testicular toxicity in male Sprague-Dawley rats. JABRA assisted reproduction. 26: 222-231. DOI: 10.5935/1518-0557.20210061.
Gundogdu G, Demirkaya Miloglu F, Gundogdu K, Tasci SY, Albayrak M, Demirci T, et al. 2020, Investigation of the efficacy of daidzein in experimental knee osteoarthritis-induced with monosodium iodoacetate in rats. 39: 2399-2408, DOI: 10.1007/s10067-020-04958-z.
Kim TI, Kwon, B, Yoon, J, Park, I-J, Bang, GS, Park, Y, et al. 2017, Antibacterial activities of graphene oxide–molybdenum disulfide nanocomposite films. 9: 7908-7917, https://doi.org/10.1021/acsami.6b12464
Krawetz, RJ, Wu, YE, Bertram, KL, Shonak, A, Masson, AO, Ren, G, et al. 2022, Synovial mesenchymal progenitor derived aggrecan regulates cartilage homeostasis and endogenous repair capacity. Cell Death & Disease. 13: 470, https://www.nature.com/articles/s41419-022-04919-1.
Kyung, BS, Jung, KW, Yeo, WJ, Seo, HK, Lee, YS, Suh, DW 2021, Differential regulation of the water channel protein aquaporins in chondrocytes of human knee articular cartilage by aging. Scientific Reports, 11: 20425, DOI: 10.1038/s41598-021-99885-7.
Lepetsos, P 2016, Papavassiliou AGJBeBA-MBoD. ROS/oxidative stress signalling in osteoarthritis. 1862: 576-591, DOI: 10.1016/j.bbadis.2016.01.003.
Li, Y, Xie, W, Xiao, W & Dou, D 2022, Progress in osteoarthritis research by the National Natural Science Foundation of China. Bone Research, 10: 41. https://www.nature.com/articles/s41413-022-00207-y.
Ma, N, Abaker, JA, Bilal, MS, Dai, H & Shen, XJBvr 2018, Sodium butyrate improves antioxidant stability in sub-acute ruminal acidosis in dairy goats. BMC Veterinary Research, 14: 1-13, DOI: 10.1186/s12917-018-1591-0.
Mobasheri, A, Rayman, MP, Gualillo, O, Sellam, J, Van Der Kraan, P & Fearon, UJNRR 2017, The role of metabolism in the pathogenesis of osteoarthritis. Nature Reviews Rheumatology, 13: 302-311, DOI: 10.1038/nrrheum.2017.50.
Panahi, Y, Alishiri, GH, Parvin, S & Sahebkar, AJJods 2016, Mitigation of systemic oxidative stress by curcuminoids in osteoarthritis: results of a randomized controlled trial. Journal of Dietary Supplements, 13: 209-20, DOI: 10.3109/19390211.2015.1008611.
Pompella, A, Visvikis, A, Paolicchi, A, De Tata, V & Casini AFJBp 2003, The changing faces of glutathione, a cellular protagonist. Biochemical Pharmacology, 66: 1499-1503. DOI: 10.1016/s0006-2952(03)00504-5.
TenBroek, EM, Yunker, L, Nies, MF & Bendele, AM 2016, Randomized controlled studies on the efficacy of antiarthritic agents in inhibiting cartilage degeneration and pain associated with progression of osteoarthritis in the rat. Arthritis Research & Therapy, 18: 1, DOI: 10.1186/s13075-016-0921-5.
Visser A, De Mutsert, R, Le Cessie, S, Den Heijer, M, Rosendaal, F & Kloppenburg, MJAotrd 2015, The relative contribution of mechanical stress and systemic processes in different types of osteoarthritis: the NEO study. Annals of the Rheumatic Diseases (ARD), 74: 1842-1847. DOI: 10.1136/annrheumdis-2013-205012.
Caspian Journal of Environmental Sciences
Volume 21, Issue 2
April 2023
Pages 403-409

Files

Share

How to cite

Statistics