Morphometric and histological characteristics of the stomach and reproductive organs in Japanese quail

Document Type : Research Paper


Peoples Friendship University of Russia (RUDN University) Moscow, Miklukho-Maklaya Street, Russia



Knowledge of the anatomical features of the digestive and reproductive systems in Japanese quails makes it possible to purposefully influence their growth and development, using breeding and selection in the right direction to preserve the health of animal and increase its productivity. Japanese quail at certain stages of postembryonic ontogenesis—neonatal (daily), juvenile (30 days), puberty (60 days), and morpho-functional maturity (90 and 240 days) were used in this study. A morphometric assessment of the stomach, ovary, and testes of Japanese quails and the development of its linear index were analysed based on the obtained data. Also, a histological study at different ages was conducted. The materials included the glandular and muscular sections of the stomach, ovaries, and testes obtained from clinically healthy Japanese quails. The material was taken in the middle of a certain stage of postembryonic ontogenesis and consisted of 10 specimens for each age group. The dissected organs of Japanese quails was weighed on an electronic balance to determine the absolute weight in grams. Next, the relative weight of the organ was calculated. Additionally, venire calipers were used to measure the studied organs length and width. In addition to studying the histological structure of the stomach, ovary, and testes. The Japanese quail's stomach was characterized by an age-related staging of formation, with the glandular part growing most intensively until 30 days and the muscular part forming only at 90 days. In the case of histological study, in the glandular stomach, the submucosal layer occupied an average of 63.03% of the total surface area on the 90th day, while the muscle layer increased by 6.32%, equivalent to an increase of 1.98 times. As for the muscular stomach. The rate of development of the inner layer of the muscular stomach varied from day 1 to day 720, increasing its surface area by 10.01 ± 0. A direct correlation between avian reproductive health, ovarian and testicular mass was recorded. Quail mature sexually between four and five weeks of age. At around 6 weeks of age, females begun to lay. In the time between 60 and 150 days of age, the ovary developed most rapidly.


Abdul Ridha, A, Salih, AN, Bargooth, AF & Wali, ON 2019, Comparative histological study of stomach in domestic pigeon (Columba liviadomestica) and domestic quail (Coturnix coturnix). Biochemical and Cellular Archives, 19: 4241-4245.
Abumandour, MM 2013, Morphological studies of the stomach of falcon, Journal of Advanced Veterinary Research, 2: 30-40.
Ahmed Abd Galil, Y, Kamel, G, Ahmad, A & Abo El Magd 2011, Histomorphological studies on the stomach of the Japanese quail. Asian Journal of Poultry Science, 5: 56-67.
Alabdallach, AZ, Nikishov, AA, Volkova, NA, Vetokh, AN, Rebouh, NY, Semenova, VI, Petrov, AK, Rystsova, EO, Bolshakova, MV, Krotova, EA & Drukovsky, SG 2020, Histological and morphometric characteristics of chicken embryos with different genotypes. EurAsian Journal of BioSciences, 14: 719-725. Corpus ID: 219109731.
Alabdallah, A, et al. 2021, "Influence of Different Genders of Japanese Quail on the Functional State of Kidneys." Archives of Razi Institute, 76: 667. DOI: 10.22092/ari.2021.355522.1694.
Alabdallah, ZA, Nikishov, AA & Karamyan, AS 2021, Sex-related of some haematological and serum biochemical changes, fed high-protein diet in Japanese quail (Coturnix japonica). Iranian Journal of Ichthyology, 8: 150-154.
Alabdallah, Z, Nikishov, A, Vatnikov, Y, Al Ragawi, A & Seleznev, S 2021, The effect of high protein in the feed mixture on the morphological changes in the kidneys of quail birds. Journal of Chemical Health Risks, 11: 383-392, DOI: 10.22034/jchr.2021.686023.
Al Saffar FJ & AlSamawy ERM 2015, Histomorphological and histochemical studies of the stomach of the mallard (Anas platyrhynchos). Asian Journal of Animal Sciences, 9: 280-292.
Al Saffar, FJ & Al Samawy ERM 2014, Microscopic and morphometric study of the proventriculus and ventriculus of the striated scope owl (Otus scors brucei) in Iraq. Kufa Journal for Veterinary Medical Sciences, 5: 9-23.
Al Tememy, H 2010, Histological study of testis in quail (Coturnix coturnix japonica). Al-Anbar Journal Veterinary Sciences, 3: 36-44.
Angel Daniel, PU, Jhonn Lenon, CJ, Laura Candelaria, CH, Alabdallah, Z & Nikishov, AA 2022, Alterations in the mass of quail eggs of different densities during storage. Caspian Journal of Environmental Sciences, 20: 1163-1166.
Asem, EK, Feng, S, Stingley Salazar, SR, Turek, JJ, Peter, AT & Robinson, JP 2000, Basal lamina of avian ovarian follicle: Influence on morphology of granulosa cells in-vitro. Comparative Biochemistry and Physiology Part C: Pharmacology, Toxicology and Endocrinology, 125: 189-201.
Attia, HF 2008, Some histological studies on the proventriculus of the quail during pre and post hatching periods. Minufiya veterinary journal, 5: 441-453.
Aughey, E & Frye, FL 2001, Comparative veterinary histology with clinical correlates. CRC Press.
Batah AL, Selman HA & Saddam, M 2012, Histological study for stomach (proventriculus and gizzard) of Coot bird (Fulica atra). Diyala Agricultural Sciences Journal4: 9-16.
Berg, C 2000, Environmental pollutants and the reproductive system in birds: developmental effects of estrogenic compounds. Acta Universitatis Upsaliensis, ISBN 9155447988.
Caceci, T 2006, Avian digestive system.
Catroxo, MHB, Lima, MAI & Cappellaro, CEMPDM 1997, Histological aspects of the stomach (proventriculus and gizzard) of the Red- Capped Cardinal (Paroariagularis gularis). Revista Chilena de Anatomía, 15: 19-27,
Cheng, KM, Bennett, DC & Mills, AD 2010, The Japanese Quail. In The UFAW Handbook on the Care and Management of Laboratory and Other Research Animals, pp. 655-673, John Wiley & Sons, Ltd.
Cooper, RG & Mahroze, KM 2004, Anatomy and physiology of the gastrointestinal tract and growth curves of the ostrich (Struthio camelus). Animal Science Journal, 75: 491-498.
Cruz, FK, Kaneko, IN, Figueroa, CDN, Bezerra, JS, ´unior, J, Craveiro, GA, Rossi, RM, Murakami, AE, Santos TC 2019, Development and growth of digestive system organs of European and Japanese quail at 14 days post-hatch. Poultry Science, 98: 1883-1892,
Dahekar, NM, Mamde, CS, John, MA & Rohankar, RU 2014, Gross Anatomical and Histomorphological Studies on Proventriculus of Japanese Quail. Indian Journal of Veterinary Anatomy, 26: 62-63.
Duke, GE 1997, Gastrointestinal physiology and nutrition in wild birds, Proceedings of the Nutrition Society. 56: 1049-1056.
Gartrell, BD 2000, The nutritional, morphologic, and physiologic bases of nectarivory in Australian birds. Journal of Avian Medicine and Surgery, 14: 85-94.
Grieser, D, de, O, Marcato, SM, Furlan, AC, Zancanela, V, Batista, E, Ton, APS, Perine, TP & Stanquevis, CE 2015, Performance and yield of carcass and parts of three different quail strains. Zootecnia Tropical, 33: 61-72, (In Portuguese).
Hassan, SA & Moussa, EA 2012, Gross and microscopic studies on the stomach of domestic duck (Anas platyrhynchos) and domestic pigeon (Columba livia domestica). Journal of Veterinary Anatomy, 5: 105-127.
Hena, SA, Sonfada, ML, Danmaigoro, A, Bello, A & Umar, AA 2012, Some comparative gross and morphometrical studies on the gastrointestinal tract in pigeon (Columbia livia) and Japanese quail (Coturnix japonica). Scientific Journal of Veterinary Advances, 1: 57-64.
Herve, T, Raphaël, KJ, Ferdinand, N, Laurine Vitrice, FT, Gaye, A, Outman, MM & Willy Marvel, NM 2018, Growth performance, serum biochemical profile, oxidative status, and fertility traits in male Japanese quail fed on ginger (Zingiber officinale, Roscoe) essential oil. Veterinary Medicine International, DOI: 10.1155/2018/7682060.
Hodges, RD 1974, The histology of the fowl. Academic Press, pp. 35-88.
Hristov, H 2021, Avian Stomach Anatomy: A Mini Review. Bulgarian Journal of Veterinary Medicine, 24: 461-468, DOI: 10.15547/bjvm.2311.
Jamieson, BG 2007, Reproductive biology and phylogeny of birds, Part B: Sexual selection, behavior, conservation, embryology and genetics. CRC Press, ISBN: 9781578084449, 542 p.
Jassem, ES, Hussein, AJ & Sawad, AA 2016, Anatomical, histological and histochemical study of the proventriculus of common Moorhen (Gallinula chloropus). Basrah Journal of Veterinary Research, 14:73-82.
Kadhim, KK, Zuki, ABZ, Noordin, MM, Babjee, SMA & Zamri Saad, M 2010, Morphological study of pancreatic duct in red jungle fowl. African Journal of Biotechnology, 9: 7209-7215.
Kadhim, KK, Zuki, ABZ, Noordin, MM & Babjee, SMA 2011, Histomorphology of the stomach, proventriculus and ventriculus of the red jungle fowl. Anatomia Histologia Embryologia, 40: 226-233.
King, DE, Asem, EK & Adeola, O 2000, Ontogenetic development of intestinal digestive functions in white pekin ducks. The Journal of Nutrition, 130: 57-62.
Kretov, AA 2018, Morphogenesis of the organs of the esophagogastric region of the Japanese quail (Coturnix coturnix japonica) under conditions of intensive use. Morphological Almanac named after V.G. Koveshnikov, 16: 28-33.
Krotova, EA, Seleznev, SB & Vetoshkina, GA 2015, Morphological aspects of reproductive system of quails. Innovative Processes in AgroIndustrial Complex, pp. 82-82.
Kühnel, W 2003, Colour atlas of cytology, histology, and microscopic anatomy. Thieme Medical Publishers. 543 p.
Langlois, I 2003, The anatomy, physiology, & diseases of the avian proventriculus & ventriculus. Veterinary Clinics of North America - Exotic Animal Practice, 6: 85-111.
Leeson, S & Summers, JD 2010, Broiler breeder production. Nottingham University Press, 338 p.
Lei, Zhu 2015, Histological and histochemical study on the stomach (proventriculus and gizzard) of black-tailed crake (Porzana bicolor). Pakistan Journal of Zoology, 47: 607-616.
Liman, N, Alan, E & Bayram, GK 2010, The differences between the localizations of Muc1, Muc5ac, Muc6 and Osteopontin on quail proventriculus and gizzard may be a reflection of functional differences of stomach parts. Journal of Anatomy, 40: 57-66.
Madkour, FA, Mohamed, AA 2018, Comparative anatomical studies on the glandular stomach of the rock pigeon (Columba livia targia) and the Egyptian laughing dove (Streptopelia senegalensis aegyptiaca). Anatomia Histologia Embryologia, 48: 1-11.
Madkour, FA 2015, Morphological studies on the stomach of the post hatching ducks (PhD Dissertation). Qena: Faculty of Veterinary Medicine, South Valley University.
Marshall, AJ & Folley, SJ 1965, The origin of nest cement in edible-nest swiftlet (Collocalia spp.). Proceedings of the Zoological Society of London, 12: 383-9.
Matias, Froilan Bernard Reyes, Dela Cruz, NAM & Salinas, MBSS 2021, "Histomorphometric analysis of mature female Japanese quail (Coturnix coturnix japonica) stomach." Iranian Journal of Veterinary Science and Technology, 13: 90-96.
McGary, S, Estevez, I, Bakst, MR & Pollock, DL 2002, Phenotypic traits as reliable indicators of fertility in male broiler breeders. Poultry Science, 81: 102-111.
Narinc, D, Aygun, A & Sari, T 2013, Effects of cage type and mating ratio on fertility in Japanese quails (Coturnix coturnix japonica) eggs. Agriculture Science Developments, 2: 4–7.
Nasrin, M, Siddiqi, MNH, Masum, MA & Wares, MA 2012, Gross and histological studies of digestive tract of broilers during postnatal growth and development. Journal of the Bangladesh Agricultural University, 10: 69-77.
Ngare, W, Ivan, D, Aspden, WJ, Robert J. Hughes, RJ, Christiansen, I, Chapman, J, Gangadoo, SH, Moore, RJ, Stanley, D 2018, Ultrastructure of the gastro intestinal tract of healthy Japanese quail (Coturnix japonica) using light and scanning electron microscopy. Animal Nutrition, 4: 378-387.
Nitsan, Z,Turro Vincent, I, Liu, G, Dunnington, EA & Siegel, PB 1995, Intubation of weight-selected chicks with soybean oil or residual yolk: Effect on early growth and development. Poultry Science, 74:925-936.
Ogunkoya, YO & Cook, RD 2009, Histomorphology of the proventriculus of three species of Australian passerines: Lichmera indistincta, Zosterops lateralis and Poephila guttata. Anatomia Histologia Embryologia, 38: 246-253.
Pesek, L, 1999, The Avian digestive tract. Winged Wisdom Pet Bird Magazine, Ezine August.
Qureshi, AS, Faisal, T, Saleemi, MK & Ali, MZ 2017, Histological and histometric alterations in the digestive tract and accessory glands of duck (Anas platyrhynchos) with sex and progressive age. Journal of Animal and Plant Sciences, 27: 1528-1533.
Rocha, DOS & Lima, MAI 1998, Histological aspects of the stomach of burrowing owl. Revista Chilena Anatomy ,16: 191-197.
Rossi JR, Baraldi Artoni, SM, Oliveira, D, da Cruz, C, Franzo, VS, Sagula, A 2005, Morphology of glandular stomach (ventriculus glandularis) and muscular stomach (ventriculus muscularis) of the partridge Rhynchotus rufescens. Ciência Rural, 35: 1319-1324.
Sales, J 2006, Digestive physiology and nutrition of ratites. Avian and Poultry Biology Reviews, 17: 41-55.
Santos, AL, Sakomura, NK, Freitas, ER, Fortes, CMS, Corilho, ENVM 2005, Comparison of free-range broiler chickens raise in confined or semiconfined systems. Brazilian Journal of Poultry Science, 7:85-92.
Schindelin, J, Arganda Carreras, I, Frise, I, Kaynig, V, Longair, M, Pietzsch, T, et al. 2012, Fiji: an open-source platform for biological-image analysis. Nature Methods, 9: 676-68.
Selvan, PS, Ushakumary, S & Ramesh, G 2008, Studies on the histochemistry of the proventriculus and gizzard of post-hatch Guinea fowl (Numida meleagris). International Journal of Poultry Science, 7: 1112-1116.
Sherri, C 2003, Avian digestive system: Holistic bird. News letter. Devoted to health and healing of avian mind, Body Spirit, 3: 44-53.
Silva, JHV, Jord˜ao Filho, J, Costa, FGP, de Lacerda, PB, Vargas, DGV& Lima, MR 2012, Nutritional requirements of quails. Revista Brasileira de Saúde e Produção Animal, 13: 775-790.
Song, Y & Silversides, FG 2006, The technique of orthotopic ovarian transplantation in the chicken. Poultry Science, 85: 1104-1106.
Starck, JM, Rahmaan, GH 2003, Phenotypic flexibility of structure and function of the digestive system of Japanese quail. Journal of Experimental Biology, 206: 1887-1897.
Subedi, K, Isobe, N & Yoshimura, Y 2008, Changes in the localization of immunoreactive avian β-defensin-12 in ovarian follicles during follicular growth and in response to lipopolysaccharide. The Journal of Poultry Science, 45: 210-214.
Suhaib, AH 2022, Morphological comparison of proventricular and gizzard in starling birds, Sturnus vulgaris and pigeon, Columba livia, International Journal of Veterinary Sciences and Animal Husbandry, 7: 15-18,
Uni, Z & Ferket, RP 2004, Methods for early nutrition and their potential. World's Poultry Science Journal, 60: 101-111.
Uni, Z, Tako, E, Gal Garber, O & Sklan, D 2003, Morpho-logical, molecular, and functional changes in the chicken small intestine of the late-term embryo. Poultry Science, 82: 1747-1754.
Wang, JX, Li, P, Zhang, XT, Ye, LX 2006, Developmental morphology study on the stomach of African ostrich chicks. Poultry Science. 96:2006-12.
Wu, B, Li, T & Yu, X 2010, Winter diet and digestive tract of the Golden pheasant (Chrysolophus pictus) in the Qinling mountains, China. Chin Birds, 1:45-50.
Zaher, M, Ghareeb, AWE, Hamdi, H & Abuamod, F, 2012, Anatomical, histological and histochemical adaptations of the avian alimentary canal to their food habits: I-Coturnix coturnix. Life Sciences, 9:253-275.
Zahra'a, D, Zainab, M & Al-samawy, R, "Histological study of the stomach in Egyptian mallard (Alopochen aegyptiaca)." European Journal of Molecular & Clinical Medicine, 7.09: 2020.
Zaima, U, Anas, SQ, Rehmatullah, Sh, Farah, D 2021, Histological and histomorphometric study of the cranial digestive tract of ostriches (Struthio camelus) with advancing age. Veterinarni Medicina, 66: 127-139,
Zhu, L, Wang, JJ, Shi, XD, Hu, J & Chen, JG 2013, Histological observation of the stomach of the Yellow-billed Grosbeak. International Journal of Morphology, 31: 512-515.