Central Sericultural Research and Training Institute, Srirampura, Mysore 570 008, India
Regional Sericultural Research Station, Anantapur -600 001, Andhra Pradesh, India
Regional Tasar Research Station, Bhandara - 441 904, Maharashtra, India
The influence of VAM fungi and bacterial biofertilizer (BBF) with 50% reduction in the recommended dose of (N and P) chemical fertilizers on leaf quality traits of mulberry variety (S-13) and its impact on silkworm (PM ? NB4D2) growth and cocoon characters were studied under semi-arid conditions. Four different treatments were imposed i.e., T1: Control (only 100% NPK); T2: VAM (50% cut in P); T3: BBF (50% cut in N) and T4: BBF and VAM (50% cut in N and P). The results revealed that reduction (50%) in the dose of chemical fertilizers in T2, T3 and T4 did not affected the leaf quality traits or cocoon parameters, this may be due to the effect of microbial inoculants in these treatments, which had efficiently regulated the normal growth, metabolism and physiological activity in plants. Among the three-biofertilizer treatments, leaf quality, silkworm growth and cocoon parameters were found improved in T4 and was on par with T1 control. The dual inoculation (T4) proved economical and beneficial with regard to saving of 50 % cost of chemical fertilizers and improvement in soil fertility, leaf quality and cocoon parameters, thus this technology can be recommended to sericulture.
Bongale, U.D. and Chaluvachari (1995) Evaluation of eight mulberry germplasm varieties by leaf biochemical and bioassay moulting studies. Sericologia 35, 83-94.
Bose, P.C. and Bindroo, B.B. (2001) A comparative biochemical study of seven promising mulberry (Morus Alba L.) varieties under rainfed conditions of subtropical region. Indian J. Seric. 40, 171 -173.
Das, P.K., Choudhury, P.C.‚ Ghosh, A.‚ Katiyar, R.S.‚ Mathur, V.B. and Datta, R.K. (1993) Use of Azotobacter biofertilizer in mulberry cultivation. Indian Silk. 3, 43 - 45.
Das, P.K., Katiyar, R.S., Hanumantha Gowda, M., Fathima, P.S. and Choudhury, P.C. (1995) Effect of vesicular arbuscular mycorrhizal inoculation on growth and development of mulberry (Morus spp) saplings Indian J. Seric., 34, 15-17.
Deckard L.‚ Lambert, R.J. and Hageman, R.H. (1973) NR activity in corn leaves as related to yields of grain and grain protein. Crop Sci. 13,343 - 350.
Dubios, M.K.‚ Giller, K.A.‚ Hamilton, K.‚ Relers, P.A. and Smith, F. (1956) Colorimetric method for determination of sugar of related substances Ann. Chem, 28, 350- 356.
Fathima, P.S., Das, P.K.‚ Katiyar, R.S.‚ Himanthraj, M.T. and Pallavi, S.N. (1996) Effect of vesicular arbuscular mychorrhiza inoculation in mulberry under different levels and sources of phosphorus on silkworm growth, cocoon yield and quality. Indian J. Seric. 35, 99-103
Gomez, K.A. and Gomez, A.A. (1983) Statistical procedure for agriculture research 2nd Edn. John Wiley and Sons, New York pp.1-680 Hiratsuka, E. (1917) Researches on the nutrition of the silkworm Shangai Shkenjo Hoko Ku., Tech Bull. 2, 353-412.
Hiscox, J.D.‚ and Israelistam G.F. (1979) A method for extraction of chlorophyll from leaf tissue without maceration. Can. J. Bot. 57, 1332-1334.
Hori, Y. (1978) Quantitative requirement of nutrition for growth of the silkworm. Bombyx mori L. JARQ. 12, 211-217.
Hori, Y. (1980) Recent advances in Sericulture. Annu. Rev. Entomol. 25, 49–71. Jackson, M.L. (1973) Nitrogen determination for soil and plant tissue. In: Soil Chemical Analysis, Prentice- Hall of India, Pvt. (Ltd.) New Delhi pp. 183-204
Kashyap, S., Sharma, S. and Vasudevan‚ P. (2004) Role of bioinoculants in developpment of salt resistant saplings of Morus alba (Var. sujanpuri) in vivo. Scientia Hort. 100, 291–307.
Katiyar, R.S., Das, P.K., Choudhury, P. C., Ghosh, A., Singh, G.B. and Datta, R.K. (1995) Response of irrigated mulberry (Morus alba L.) to VA mycorrhiza inoculation under graded doses of Phosphorus. Plant Soil. 170, 331-337.
Krishnaswami, S. (1978) New Technology of Silkworm rearing. Bulletin CSRI 2, 1-24 Lowri, O.H.‚ Rosenbrough, N.J., Farr, A.L. and Randall, R.J. (1951) Protein measurement with folin phenol reagent. J. Biol. Chem. 193, 265- 275.
Machii, H. (1989) Varietal differences of nitrogen and amino acid contents in mulberry leaves. Acta Sericul. Entomol. 1, 51-61. Machii, H. and Katagiri, K. (1991) Varietal differences in nutritive values of mulberry leaves for rearing silkworm. JARQ. 25, 202- 208.
Mandhare, V.K., Patil, P.C.‚ Gadekar, D.A. (1998) Phosphorus uptake of onion as influenced by Glomus fasiculatum, Azotobacter and phosphorus levels. Agric. Sci. Digest. 18, 228–230.
Marwaha, B.C. (1995) Biofertilizer – A supplementary source of plant nutrient. Fertilizer News. 40, 39-50.
McCready, R.M., Guggale, J., Silver, V. and Owens, H.S. (1960) Determination of starch and amylase in vegetables. Application to Peas, Anal Chem., 29, 1156-1158.
Panwar, J.D.S. (1993) Response of VAM and Azospirillum inoculation to water status and grain yield in wheat under stress condition. Indian J. Plant Physiol. 36, 41-43.
Rahmathulla, V.K., Himanthraj, M.T.‚ Srinivasa, G. and Rajan, R.K. (2004). Association of moisture content in mulberry leaf with nutritional parameters of bivoltine silkworm (Bombyx mori L.) Acta Entomol. Sinica. 47, 701-704.
Ram Rao, D.M., Reddy, M.P.‚ Reddy, B.K. and Suryanarayana N. (2000) Nitrate reductase (NR) activity and its relationship with protein content, leaf yield and its components in mulberry (Morus spp.). Indian J. Seric. 39, 86-88.
Reddy, M.P., Rao, D.M.R.‚ Verma, R.S.‚ Srinath, B. and Katiyar, R.S. (1998) Response of S13 mulberry variety to VAM inoculation under semi-arid condition Indian J. Plant Physiol. 3,194-196
Reddy, M.P., Ram Rao, D.M.‚ Katiyar, R.S. and Suryanarayana, N. (2000b) Economisation of N and P fertilizers through dual inoculation of VAM and Bacterial biofertilizers in farmers field of Andhra Pradesh. Proceedings of the National Conference on Strategies for Sericulture Research and Development, 16 – 18 November 2000. Adv. in Indian Seri. Res., pp. 212-215.
Sansamma, G., Raghavah, P. and Pushpakumari, R. (1998) Influence of biofertilizer on productivity of guinea grass intercropped in coconut garden, Indian J. Agron. 43,622-627.
Scot, D.B. and Neyra, C.A. (1979) Glutamine synthetase and Nitrate assimilation in sorghum leaves Can. J. Bot 57, 754-758
Selvaraj, T., Sivakumar, P. and Bhaskar, C. (1996) Comparative efficiency of different VAM fungi on Coleus aromaticus (Benth) and Coleus barbatus (Benth). J. Indian Bot. SOC., 75,271-273.
Sharma, S.D., Bhutan, V.P. and Awasthi, R.P. (2002) Effect of Vesicular arbuscular mycorrhiza and phosphorus on leaf and soil mineral nutrient status of apple seedlings. Indian J. Hort. 59, 141-144.
Spies, J.R. (1955) Colorimetric procedure for amino acids and phenol In: Methods in Enzymol. Colonick, S.P. and N.P.O. Kaplan (Eds.) Vol 3, 461- 477.
Sreeramulu, K.R., Hanumanthappa, M. Gowda, A.‚ Kalyana, K. N.‚ Jayasheela, N. (2000) Dual inoculation of Azotobacter chrococcum and Glomus fasciculatum improves growth and yield of sunflower under field conditions and saves N and P fertilizer application Environ. Ecol. 18, 380- 384.
Subba Rao, N.S. (1982) Biofertilizers in Agriculture. Oxford/ IBH publishing Co. Pvt. Ltd. New Delhi, India. Sujathamma, P. and Dandin, S.B. (2000) Leaf quality evaluation of mulberry (Morus spp.) through chemical analysis Indian J. Seric. 39, 117-121.
Sumana, D.A. and Bagyaraj, D.J. (2002) Interaction between VAM fungi and Nitrogen fixing bacteria and their influence on growth and nutrition of neem (Azadirachta indica A Juss). Indian J. Microbiol. 42,295-298.
Suryanarayana, N and Shivashankar Murthy, T.C. (2002) Differences in amino acid contents in leaf blades of mulberry (Morus spp.) Varieties. Adv. Plan Sci. 15, 475–481.
Thakur, A.K. and Panwar, J.D.S. (1995) Effect of Rhizobium-VAM interaction on growth and yield in Mung bean (Vigna Radiata L.) Wilezek. Under field conditions. Indian J. Plant Physiol. 38, 62–65.
Umakanth, C.G. and Bagyaraj, D.J. (1998) Response of mulberry saplings to inoculation with VA mycorrhizal fungi and Azotobacter, Sericologia, 38, 669-675.
Vijayan, K., Tikader, A.‚ Das, K.K.‚ Roy, B.N. and Pawan Kumar T. (1996) Genotypic influence on leaf moisture content and moisture retention capacity in mulberry Morus spp. Bull. Seri. Res. 7, 95-98.