Department of Plant Protection,Faculty of Agriculture, University of Guilan, Rasht,
Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, Alberta, , T6G 2P5
A study was conducted to assess the durable resistance in a near isogenic line of spring wheat (Triticum aestivalis L.), possessing resistance gene Yr-18 to some isolates (race specific resistance) of stripe rust (Puccinia striiformis f. sp. tritici), namely Thatcher Yr-18 and durable resistance of an cultivar of spring wheat to all isolates of stripe rust (race non- specific resistance), namely Hybrid de Berse'e. In this investigation fresh urediniospores of two isolates namely SRC 99 (race 70E 128) and SRC 89 (race 14E 14), were collected from a susceptible cultivar of spring wheat, Avocet, as inoculums. Then suspension of spore in mineral oil [Soltrol 170 (5 mg/ml)] were sprayed on third and flag leaves of the booth genotypes mentioned above and on the Thatcher cultivar which was susceptible to booth isolates. The Percentage of urediniospore germination, latent period and infection types of all susceptible and resistant genotypes to booth isolates were determined in four replicates at seedling and adult plant stages. Mean percentage of spore germination and the value of latent period were analyzed separately in each replicate, and then compared using analysis of variance and the Student- Newman Keuls test. The results indicated that urediniospoe germination in all genotypes / isolates/ leaf stage combination was high and neither resistance type affected spore germination significantly. In both types of resistance, latent period increased with advancing plant age. In general cultivars possessing resistant gene had longer latent periods. Assessment of the infection type showed that, the durable resistance of Hybrid de Bers?e is detected at an earlier stage than the adult-plant resistance of near isogenic line containing the gene Yr18.