Effects of glucose and fructose on the growth and recombinant transferrin production in Pichia pastoris

Articles in Press

Document Type : Research Paper

Authors

Department of Biology, SR.C., Islamic Azad University, Tehran, Iran

10.22124/cjes.2026.9382

Abstract

ABSTRACT
This study aimed to evaluate the impact of the carbon sources glucose and fructose on the growth of recombinant Pichia pastoris harboring the human transferrin gene, which was codon-optimized, cloned into the pPICZαB vector, and integrated into the genome of P. pastoris strain GS115. The resulting recombinant strain was cultivated in defined media using either glucose or fructose as the sole carbon source. Cell growth was monitored over time by measuring optical density at 600 nm (OD₆₀₀). Our results demonstrated that the carbon source significantly influenced growth kinetics and final biomass yield. Cultures supplemented with fructose achieved a higher maximum cell density and exhibited faster growth rates compared to those cultivated on glucose. This study identifies fructose as the superior carbon source for promoting the growth of recombinant Pichia pastoris, providing a crucial physiological foundation for optimizing the cultivation process of this engineered yeast strain.

Keywords

References

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Caspian Journal of Environmental Sciences

Articles in Press, Corrected Proof
Available Online from 25 January 2026

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