Effects of Haematococcus pluvialis green alga extract on the expression of p53 and Bcl-2 genes in the MDA-MB-231 human breast cancer cell line

Document Type : Research Paper

Authors

Department of Biology, Faculty of Basic Sciences, University of Guilan, Rasht, Iran

10.22124/cjes.2025.9260

Abstract

Seaweeds are a natural source of a wide range of secondary metabolites with different biological activities, including anticancer activity. The present study aimed to investigate the toxicity of aqueous and organic extracts of the Haematococcus pluvialis green microalgae to varying concentrations on the MDA-MB-231 breast cancer cell line and to investigate the expression of genes involved in the apoptosis pathway, Bcl-2, and p53. The toxic effect of different concentrations (50, 100, 150, and 200 μg mL-1) of aqueous, methanolic, hexane, and chloroform extracts of the microalgae was measured by the MTT method, and then the expressions of p53 and Bcl-2 genes were evaluated by the RT-qPCR method. The percentage of cancer cell viability was inversely related to the concentration of the extracts and the treatment time. Among the extracts, the hexane extract of microalgae at a concentration of 200 µg mL-1 showed the highest cytotoxic effect (18%) compared to other extracts. Also, the expression of Bcl-2 and p53 genes in the treatment with hexane extract significantly decreased and elevated compared to the control and aqueous extract, respectively. As a result, the toxicity effect of the hexane extract of H. pluvialis against MDA-MB-231 breast cancer cells was efficient, so it can be a basis for further studies to introduce this green microalga as a suitable candidate for a biological compound with anticancer activity.

Keywords

References

Alateyah, N, Ahmad, S, Gupta, I, Fouzat, A, Thaher, MI, Das, P, Al Moustafa, AE & Ouhtit, A 2022, Haematococcus pluvialis microalgae extract inhibits proliferation, invasion, and induces apoptosis in breast cancer cells. Frontiers in Nutrition, 9, 882956.
Boussiba, S 2000, Carotenogenesis in the green alga Haematococcus pluvialis: cellular physiology and stress response. Physiologia Plantarum, 108: 111-117.
Bradford, MM 1976, A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72: 248-254.
Burits, M & Bucar, F 2000, Antioxidant activity of Nigella sativa essential oil. Phytotherapy Research, 14: 323-328.
Cetraro, P, Plaza-Diaz, J, MacKenzie, A & Abadía-Molina, F 2022, A review of the current impact of inhibitors of apoptosis proteins and their repression in cancer. Cancers, 14: 1671.
Chandler, D, El-Naggar, AK, Brisbay, S, Redline, RW & McDonnell, TJ 1994, Apoptosis and expression of the bcl-2 proto-oncogene in the fetal and adult human kidney: evidence for the contribution of bcl-2 expression to renal carcinogenesis. Human Pathology, 25: 789-796.
Dakubo, GD 2010, The Warburg phenomenon and other metabolic alterations of cancer cells. Mitochondrial Genetics and Cancer, 39-66.
Dellai, A, Laajili, S, Le Morvan, V, Robert, J & Bouraoui, A 2013, Antiproliferative activity and phenolics of the Mediterranean seaweed Laurencia obusta. Industrial Crops and Products, 47: 252-255.
Dere, S, Gunes, T & Sivaci, R 1998, Spectrophotometric determination of chlorophyll-A, B and totalcarotenoid contents of some algae species using different solvents. Turkish Journal of Botany, 22: 13-18.
El-Deiry, WS, Tokino, T, Velculescu, VE, Levy, DB, Parsons, R, Trent, JM, Lin, D, Mercer, WE, Kinzler, KW & Vogelstein, B 1993, WAF1, a potential mediator of p53 tumor suppression. Cell, 75: 817-825.
Ercolano, G, De Cicco, P & Ianaro, A 2019, New drugs from the sea: Pro-apoptotic activity of sponges and algae derived compounds. Marine Drugs, 17: 31.
Fulda, S & Debatin, KM 2006, Extrinsic versus intrinsic apoptosis pathways in anticancer chemotherapy. Oncogene, 25: 4798-4811.
Gyulkhandanyan, AV, Mutlu, A, Freedman, J & Leytin, V 2012, Markers of platelet apoptosis: methodology and applications. Journal of Thrombosis and Thrombolysis, 33: 397-411.
Hanahan, D 2022, Hallmarks of cancer: new dimensions. Cancer Discovery, 12: 31-46.
Hemasudha, T, Thiruchelvi, R & Balashanmugam, P 2019, Antioxidant, antibacterial, and anticancer activity from Marine Red Algae Gracilaria edulis. Asian Journal of Pharmaceutical and Clinical Research, 12: 276-279.
Kawsi, M & Fatemimoghadam, D 2019, Effect of Spirulina microalgae extract on the expression of anti-apoptotic Bcl2 gene in brain cancer cell line. Pars Journal of Medical Sciences, 17: 17-25.
Li, X, Wang, X, Duan, C, Yi, S, Gao, Z, Xiao, C, Agathos, SN, Wang, G & Li, J 2020, Biotechnological production of astaxanthin from the microalga Haematococcus pluvialis. Biotechnology Advances, 43, 107602.
Li, Y, Huang, W, Huang, S, Du, J & Huang, C 2012, Screening of anti-cancer agent using zebrafish: comparison with the MTT assay. Biochemical and Biophysical Research Communications, 422: 85-90.
Lim, S, Cheung, P, Ooi, V & Ang, P 2002, Evaluation of antioxidative activity of extracts from a brown seaweed, Sargassum siliquastrum. Journal of Agricultural and Food Chemistry, 50: 3862-3866.
Mourya, M, Khan, MJ, Sirotiya, V, Ahirwar, A, Schoefs, B, Marchand, J & Vinayak, V 2023, Enhancing the biochemical growth of Haematococcus pluvialis by mitigation of broad-spectrum light stress in wastewater cultures. RSC advances, 13: 17611-17620.
Naderi Farsani, M, Meshkini, S & Manaffar, R 2015, Study of optimal growth conditions and nutritional value of two native microalgae, Haematococcus and Desmodesmus conatus in different culture media. Scientific-Research Quarterly Journal of Microorganism Biology, 14: 49-60.
Namvar, F, Baharara, J & Mahdi, A 2014, Antioxidant and anticancer activities of selected Persian Gulf algae. Indian Journal of Clinical Biochemistry, 29: 13-20.
 
Namvar, F, Mohamad, R, Baharara, J, Zafar-Balanejad, S, Fargahi, F & Rahman, HS 2013, Antioxidant, antiproliferative, and antiangiogenesis effects of polyphenol-rich seaweed (Sargassum muticum). BioMed Research International, 2013: 604787.
Palozza, P, Torelli, C, Boninsegna, A, Simone, R, Catalano, A, Mele, MC & Picci, N 2009, Growth-inhibitory effects of the astaxanthin-rich alga Haematococcus pluvialis in human colon cancer cells. Cancer Letters, 283: 108-117.
Park, WS, Kim, HJ, Li, M, Lim, DH, Kim, J, Kwak, SS, Kang, CM, Ferruzzi, MG & Ahn, MJ 2018, Two classes of pigments, carotenoids and c-phycocyanin, in spirulina powder and their antioxidant activities. Molecules, 23: 2065.
Taheri, A, Ghafari, M, Bavi, Z & Soheili, F 2018, Cytotoxic effect of Sargassum glaucescens seaweed extract against breast (MCF7) and colorectal (HT-29) cancer cell lines. Feyz Medical Sciences Journal, 22: 292-301.
Tripathi, U, Sarada, R, Rao, SR & Ravishankar, G 1999, Production of astaxanthin in Haematococcus pluvialis cultured in various media. Bioresource Technology, 68: 197-199.
Vahdat, S, Atashbar, B, Biabani-Asrami, M & Nouri, F 2017, Combined effect of chemical fertilizers and BG11 culture medium on growth rate, biochemical composition of chlorophyll and total carotenoid content of Haematococcus pluvialis algae in brackish water. Journal of Aquaculture Sciences, 5: 27-36.
Van Loo, G, Saelens, X, Van Gurp, M, MacFarlane, M, Martin, S & Vandenabeele, P 2002, The role of mitochondrial factors in apoptosis: a Russian roulette with more than one bullet. Cell Death & Differentiation, 9: 1031-1042.
Caspian Journal of Environmental Sciences
Volume 23, Issue 5
Special issue: Biological Researches and Envireonment, Guest Editor: Prof. Hamed Mousavi-Sabet, University of Guilan, Iran
December 2025
Pages 1303-1311

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