Comparative study of the composition and biological activity of polyphenols of currants, raspberries and strawberries

Document Type : Research Paper

Authors

1 Department "Technology and standardization", JSC "K. Kulazhanov kazakh university of technology and business», Astana, Kazakhstan

2 Department "Microbiology and virology named after Sh. Sarbasova”, NJSC "Medical University Astana”, Astana, Kazakhstan

3 Laboratory of the first city hospital, R. Koshkarbaev, Astana, Kazakhstan

4 Department of Chemistry and Chemical Technology, K. Zhubanov Aktobe Regional University, Aktobe, Kazakhstan

5 Department of Food Biotechnology, Almaty Technological University, Almaty, Kazakhstan

10.22124/cjes.2025.9207

Abstract

Berry fruits are of great nutritional and medicinal importance due to their polyphenolic compounds. The current research provided a comparative study of the polyphenolic compounds and bioactive activities of three fruits, currants (grapes), raspberries and strawberries, cultivated in the Almaty region of Kazakhstan. Polyphenolic compounds were determined using high-performance liquid chromatography and antioxidant activity was determined using two assays, DPPH and FRAP, and also for the alpha-glucosidase and acetylcholinesterase enzyme inhibitory activities. Quantitative analysis indicated that there were significant differences in the polyphenolic profile of these fruits. Total polyphenol content in strawberry (520.6 mg g-1 dry weight) was significantly higher than raspberry (452.8) and currant (385.5). Strawberries had a very high content of this compound, i.e., 223 mg/100 g dry weight of anthocyanins. Raspberries, however, had the highest content of ellagitannins (158.4 mg/100 g dry weight) and had the highest DPPH free radical inhibitory activity (IC₅₀ value of 38.5 μg mL-1). Currants were also the richest in these compounds and had 1.151 mg/100 g dry weight of flavonols. In the enzyme inhibition test, raspberries were the most potent inhibitors of the enzymes alpha-glucosidase (IC₅₀ = 0.65 mg mL-1) and acetylcholinesterase (IC₅₀ = 1.15 mg mL-1). The results of this research clearly show that all these fruits possess a unique chemical fingerprint and hence have varying health-promoting potential, which can be utilized as a scientific basis for the development of targeted nutraceutical products.

Keywords

References

Aatif, M 2023, Current Understanding of Polyphenols to Enhance Bioavailability for Better Therapies. Biomedicines, 11(7): 30-45.
Abbas, M, Saeed, F, Anjum, F, Afzaal, M, Tufail, T, Bashir, MS, Ishtiaq, A, Hussain, S & Suleria, H 2017, Natural polyphenols: An overview. International Journal of Food Properties, 20(8): 1689-1699. https://doi.org/10.1080/10942912.2016.1220393.
Alkhatib, A, Tsang, C, Tiss, A, Bahorun, T, Arefanian, H, Barake, R, Khadir, A & Tuomilehto, J 2017, Functional foods and lifestyle approaches for diabetes prevention and management. Nutrients, 9(12): 1310-1324.
Ávila, F, Theoduloz, C, López-Alarcón, C, Dorta, E & Schmeda-Hirschmann, G 2017, Cytoprotective mechanisms mediated by polyphenols from chilean native berries against free radical-induced damage on AGS Cells. Oxidative Medicine and Cellular Longevity, 5(2): 12-53. https://doi.org/10.1155/2017/9808520.
Bristy, A T, Islam, T, Ahmed, R, Hossain, J, Reza, H & Jain, P 2022, Evaluation of total phenolic content, HPLC analysis, and antioxidant potential of three local varieties of mushroom: A comparative study. International Journal of Food Science, 14(3): 38-47. https://doi.org/10.1155/2022/3834936.
Coşkun, N, Sarıtaş, S, Bechelany, M & Karav, S 2025, Polyphenols in foods and their use in the food industry: Enhancing the quality and nutritional value of functional foods. International Journal of Molecular Sciences, 26(12), 58-71.
Djordjević, B 2023, Phenolics compounds in fruits of different types of berries and their beneficent for human health. Annals of the University of Craiova - Agriculture Montanology Cadastre Series, 53(1): 122-129.
Dueñas, M, Muñoz-González, I, Cueva, C, Jiménez-Girón, A, Sánchez-Patán, F, Santos-Buelga, C, Moreno-Arribas, MV & Bartolomé, B 2015, A survey of modulation of gut microbiota by dietary polyphenols. BioMed Research International, 17(4): 85-92. https://doi.org/10.1155/2015/850902.
Georgescu, C, Frum, A, Virchea, L-I, Sumacheva, A, Shamtsyan, M, Gligor, F, Oláh, N, Máthé, E & Mironescu, M 2022, Geographic variability of berry phytochemicals with antioxidant and antimicrobial properties. Molecules, 27(15): 49-63. https://doi.org/10.3390/molecules27154986.
Hammood, SS, Khleel, AI & Manea, AI 2025, Influence of organic extracts and biofertilizers on romanesco cauliflower performance indicators, Procedia Environmental Science, Engineering and Management, 12 (2): 459-466.
Kierońska, E, Skoczylas, J, Dziadek, K, Pomietło, U, Piątkowska, E & Kopeć, A 2024, Basic chemical composition, selected polyphenolic profile and antioxidant activity in various types of currant (Ribes spp.) Fruits. Applied Sciences, 14(19): 82-88.
Kschonsek, J, Wolfram, T, Stöckl, A & Böhm, V 2018, Polyphenolic compounds analysis of old and new apple cultivars and contribution of polyphenolic profile to the in vitro antioxidant capacity. Antioxidants, 7(1): 20-34.
Mikulič-Petkovšek, M, Slatnar, A, Stampar, F & Veberič, R 2012, HPLC-MSn identification and quantification of flavonol glycosides in 28 wild and cultivated berry species. Food Chemistry, 135(4): 2138-2146.
Mishra, A, Gupta, AK, Singh, V, Srivastava, R, Baboo, A & Katiyar, P 2025, Abiochemical approach to cardiac risk: association of AST & ALT levels with myocardial infarction in Type 2 Diabetes Mellitus. Revista Latinoamericana de Hipertension, 20(6), 443-448, http://doi.org/10.5281/zenodo.15741708.
Niu, J, Shang, M, Li, X, Sang, S, Chen, L, Long, J, Jiao, A, Ji, H, Jin, Z & Qiu, C 2023, Health benefits, mechanisms of interaction with food components, and delivery of tea polyphenols: A review. Critical Reviews in Food Science and Nutrition, 63(33): 45-64.
Rachmawaty R, Kasifah K, Nugrohowati N, Mediastari A, Jumintono J, Susilo H 2025, Increasing food health and safety by improving biological fertility and using organic agriculture. Procedia Environmental Science, Engineering and Management, 12(2): 231-236.
Staszowska-Karkut, M & Materska, M 2020, Phenolic composition, mineral content, and beneficial bioactivities of leaf extracts from black currant (Ribes nigrum L.), raspberry (Rubus idaeus), and aronia (Aronia melanocarpa). Nutrients, 12(4), 463-474.
Xiao, T, Guo, Z, Sun, B & Zhao, Y 2017, Identification of anthocyanins from four kinds of berries and their inhibition activity to α-glycosidase and protein tyrosine phosphatase 1B by HPLC-FT-ICR MS/MS. Journal of Agricultural and Food Chemistry, 65(30): 211-223. https://doi.org/10.1021/acs.jafc.7b02550.
Yan, Y, Pico, J, Sun, B, Pratap-Singh, A, Gerbrandt, E & Castellarin, SD 2021, Phenolic profiles and their responses to pre- and post-harvest factors in small fruits: a review. Critical Reviews in Food Science and Nutrition, 61(8): 86-96.
Zhang, L, McClements, DJ, Wei, Z, Wang, G, Liu, X & Liu, F 2020, Delivery of synergistic polyphenol combinations using biopolymer-based systems: Advances in physicochemical properties, stability and bioavailability. Critical Reviews in Food Science and Nutrition, 60(12): 83-97.
Zhang, Z, Li, X, Sang, S, McClements, D J, Chen, L, Long, J, Jiao, A, Jin, Z, & Qiu, C 2022, Polyphenols as plant-based nutraceuticals: Health effects, encapsulation, nano-delivery, and application. Foods, 11(15): 21-32.
Żurek, N, Pawłowska, A & Kapusta, I 2023, Obtaining preparations with increased content of bioactive compounds from eight types of berries. Journal of Berry Research, 13(2): 137-152. https://doi.org/10.3233/JBR-230020.
Caspian Journal of Environmental Sciences
Volume 23, Issue 4
October 2025
Pages 903-910

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