Phytosanitary diagnostics of apple powdery mildew, Podosphaera leucotricha and apple scab, Venturia inaequalis

Document Type : Research Paper

Authors

1 Abai Kazakh National Pedagogical University, Dostyk st.13, 050010, Almaty, Kazakhstan

2 Kazakh Fruit and Vegetable Research Institute, Gagarin st. 238/5, 050060, Almaty, Kazakhstan

3 Central Asian Innovative University, Madeli khozha st. 137, 160023, Shymkent, Kazakhstan

4 Toraighyrov University, Lomov st.46, 140008, Pavlodar, Kazakhstan

5 L.N. Gumilyov Eurasian National University, Satpaeva st. 2, 010008, Astana, Kazakhstan

6 M. Auezov South Kazakhstan University, Tauke Khan st.5,160009, Shymkent, Kazakhstan

10.22124/cjes.2025.9205

Abstract

The article presents the results of phytosanitary monitoring of apple orchards in the Almaty region, Kazakhstan conducted in 2023. The aim of the study was to determine the distribution and development level of major fungal diseases of apple trees – scab, Venturia inaequalis and powdery mildew, Podosphaera leucotricha – under the conditions of Southern and Southeastern Kazakhstan. Surveys were carried out in the orchards of “Koram” farm (Koram village, Enbekshikazakh district), where nine apple cultivars (Gala, Fuji, Golden Delicious, Quinti, Red Delicious, Shaphida, Korean, and Stark Earliest) were grown on an area of 110 ha. For the phytosanitary assessment, 95–100 trees of each cultivar were examined, and 2500–3000 leaves per cultivar were analyzed. The results revealed cultivar-specific susceptibility to fungal pathogens. Scab infection was recorded in Gala, Golden Delicious, and Stark Earliest (15.0–22.0% prevalence with a low development level of 0.4–1.3%). Symptoms of powdery mildew were observed in Gala, Fuji, Golden Delicious, and Red Delicious, where the prevalence reached 30.0–33.3%, while the disease development remained weak (0.3–1.5%). No symptoms of either disease were detected in the Quinti, Shaphida, and Korean cultivars. Thus, Gala, Golden Delicious, and Red Delicious were identified as the most susceptible cultivars, whereas Quinti, Shaphida, and Korean demonstrated relative resistance to scab and powdery mildew. The findings confirm the necessity of systematic phytosanitary monitoring of orchards and may serve as a scientific basis for breeding programs and for the introduction of resistant apple cultivars in the southern regions of Kazakhstan.

Keywords


Bus, VGM, Ranatunga, C, Alspach, PA, Oraguzie, NC & Whitworth, C 2006, A partial diallel study of powdery mildew resistance in six apple cultivars under three growing conditions with different disease pressures.  Euphytica. 148 (3): 235-242, https://doi.org/10.1007/s10681-005-9014-2.
Chevalier, M, Lespinasse, Y & Renaudin, S 1991, A microscopic study of the different classes of symptoms coded by the Vf gene in apple for resistance to scab (Venturia inaequalis). Plant Pathology,  40 (2): 249-256, https://doiorg/101111/j1365-30591991tb02374x.
Cipriani, G, Lot, G, Huang, W-G, Marrazzo, MT, Peterlunger, E & Testolin, R 1999, AC/GT and AG/CT microsatellite repeats in peach [Prunus persica (L) Batsch]: isolation, characterisation and cross-species amplification in Prunus. Theoretical and Applied Genetics, 99: 65-72,  https://doiorg/101007/s001220051209.
Di Gaspero, G, Peterlunger, E, Testolin, R, Edwards, KJ & Cipriani, G 2000, Conservation of microsatellite loci within the genus Vitis. Theoretical and Applied Genetics, 101: 301-308, https://doiorg/101007/ s001220051483.
Evans, K & James, C 2003, Identification of SCAR markers linked to Pl-w mildew resistance in apple. Theoretical and Applied Genetics, 106: 1178-1183, https://doiorg/101007/s00122-002-1147-2.
Galymbek, K, Madenova, A & Bakirov, SB 2024, Phytosanitary diagnosis of apple fungal diseases in Almaty region. Research on Crops, 25: 499-508,  http://dxdoiorg/1031830/2348-75422024ROC-1112.
Galymbek, K, Kuan, A, Kaldybayeva, D, Abdikarimova, R, Bakirov, S, Aitymbe,t Z & Madenova, A 2025, Distribution of powdery mildew resistance genes in the Kazakhstan apple cultivar collection. Brazilian Journal of Biology, 85: e287949  https://doiorg/101590/1519-6984287949.
Gessler, C, Patocchi, A, Sansavini, S, Tartarini, S & Gianfranceschi, L 2006, Venturia inaequalis resistance in apple. Critical Reviews in Plant Sciences, 25 (6):  473-503 https://doiorg/101080/07352680601015975.
Gross, BL, Kellogg, EA & Miller AJ 2014, Speaking of food: Connecting basic and applied plant science. American Journal of Botany, 101 (10): 1597-1600, https://doi.org/10.3732/ajb.1400409.
Hokanson, SC, Szewc-McFadden, AK, Lambo,y WF & McFerson, JR 1998, Microsatellite (SSR) markers reveal genetic identities, genetic diversity and relationships in a Malus× domestica Borkh core subset collection  Theoretical and Applied Genetics,  97: 671-683,  https://doiorg/101007/s001220050943.
Khajuria, YP, Kaul, S, Wani, AA & Dhar, MK 2018, Genetics of resistance in apple against Venturia inaequalis (Wint) Cke. Tree Genetics & Genomes, 14: 1-20, https://doiorg/101007/s11295-018-1226-4.
Madenova, A, Aitymbet, Z, Bolat, M, Kaldybayeva, D, Galymbek, K, Kuan, A, Kabylbekova, B, Irkitbay, A, Yeszhanov, T, Bakirov, S & Sapakhova, Z 2024, Screening of apple cultivars for scab resistance in Kazakhstan Horticulturae, 10: 184 https://doiorg/103390/horticulturae10020184.
Nasonov, AI & Suprun, I I 2015, Apple scab: peculiarities of the causal agent and the pathogenesis. pp. 275-285.
Pessina, S, Angeli, D, Martens, S, Visser, RGF, Bai, Y, Salamini, F, Velasco, R, Schouten, HJ & Malnoy, M 2016, The knock‐down of the expression of MdMLO19 reduces susceptibility to powdery mildew (Podosphaera leucotricha) in apple (Malus domestica). Plant Biotechnology Journal, 14 (10): 2033-2044, https://doiorg/ 101111/pbi12562.
Savelyev, NI, Savelyeva, NN & Yushkov, AN 2009, Promising apple tree varieties immune to scab. Monograph. Michurinsknaukograd, 126 p, [In Russian].
Soriano, JM, Joshi, SG, van Kaauwen, M, Noordijk, Y, Groenwold, R, Henken, B, van de Weg, WE & Schoutenet, HJ 2009, Identification and mapping of the novel apple scab resistance gene Vd3. Tree Genetics & Genomes, 5: 475-482, https://doi.org/10.1007/s11295-009-0201-5.
Vallée Marcotte, B, Verheyde, M, Pomerleau, S, Doyen, A & Couillard, C 2022, Health benefits of apple juice consumption: A review of interventional trials on humans. Nutrients, 14: 821.
Weber, JL & May, PE 1989, Abundant class of human DNA polymorphisms which can be typed using the polymerase chain reaction. American Journal of Human Genetics, 44 (3): 388.
Xu, Y & Crouch, JH 2008, Marker‐assisted selection in plant breeding: From publications to practice. Crop Science, 48 (2): 391-407, https://doi: 102135/ cropsci2007040191.
Yakuba, GV 2013, The study of the main trends in the development of mycoses in changing environmental conditions. Fruit growing and berry growing in Russia, Collection of scientific works, 26 (2): 355-360.
Zhdanov, VV 1991, Selekcija jabloni na ustojchivost'k parshe. [Apple tree breeding for scab resistance]. Tula: Priok Book Publishing House [Tula: Priok kn izd-vo] [In Russian].