Confirming cosmetics products made with caviar extract using the Syber Green Quantitative PCR technique

The verification of biomaterial used in cosmetic products needs to be tested through some techniques such as hybridization, DNA-binding protein, fluorescence dye, and quantitative PCR, all based on identifying protein or DNA remaining in the product. In this research, a real-time PCR method was used to evaluate the remaining DNA of caviar extract, which is based on the use of Syber ​​Green buffer. At first, DNA extraction was performed from the fin tissue of the Caspian Sea sturgeons, including Siberian and Sterlet sturgeons, as a model for fragment amplification. So, a small cytochrome oxidase Ι gene fragment was amplified using the conventional PCR. To verify the presence of DNA in caviar extract using the real-time PCR method, a standard curve was drawn using the DNA of the fin tissue of Siberian sturgeon. The primer specificity for sturgeons was tested using negative control DNA like human, yeast, and bacteria. Results showed that all seven sturgeons amplified a fragment of CoΙ gene with specific sturgeon primers. A standard curve was drawn with a linear relationship between threshold cycle values, and a coefficient higher than 98% was obtained with the real-time PCR method. According to the standard curve, unknown samples of caviar cream DNA concentration ranges were 2 ng - 400 pg. None of the irrelevant genomic DNA (human, yeast, and bacteria) was indistinguishable from the response of the negative control when analyzed with the COI sturgeon's specific primer. In this research, real-time PCR could detect picogram and nanogram levels of residual DNA in cosmetic products, and the verification of caviar cream was approved for making it with caviar extract using this sensitive technique.