Effects of chemical and biological phosphorus fertilizers on the activity of antioxidant enzymes and some biochemical traits of cumin

Document Type : Research Paper

Authors

1 DSc, Professor of the Department of Faculty and Hospital Surgery. Bukhara State Medical Institute named after Abu Ali Ibn Sino, Bukhara, Uzbekistan

2 Department of Zoology and General Biology, Faculty of Natural Sciences, Fergana State University, 19 Murabbilar St., Fergana 150100, Uzbekistan

3 Department of Botany, Termiz State University, 190110 Termiz, Surkhandaryo, Uzbekistan

4 Course leader "Beauty Aesthetics", Kimyo International University in Tashkent, Tashkent, Uzbekistan

5 Tashkent Institute of Irrigation and Agricultural Mechanization Engineers” National Research University, Tashkent, Uzbekistan; Alfraganus University, Yukori Karakamish Street 2a, Yunusabad District, Tashkent, Uzbekistan; cWestern Caspian University Baku, Istiglaliyyet 31, Baku, Azerbaijan

6 Associate Professor, Doctor of Philosofy of in Biological Sciences (PhD), Karshi State University, Karshi City, Uzbekistan

7 PhD in Theory and Methodology of Education and Upbringing (Biology), Associate Professor, Karshi State University, 180119, Karshi, Uzbekistan

8 DSc, Associate Professor, Head of the Department of Clinical Laboratory Diagnostics with the Course of Clinical Laboratory Diagnostics of the PGD, Samarkand State Medical University,140100, Samarkand, Uzbekistan

9 Associate Professor, Department of Fruit and Vegetable Growing, Urgench State University, Urgench, Uzbekistan

10 Associate Professor, Mamun University, Uzbekistan

10.22124/cjes.2025.8951

Abstract

In this research on the effect of chemical and biological phosphorus fertilizers on antioxidant enzyme activity and biochemical characteristics of cumin, a factorial experiment was conducted in a complete randomized design over three replications in pot experiments. Treatment consisted of three levels of triple superphosphate chemical fertilizer (0, 50, and 100 kg ha-1) and two levels of biofertilizer (no inoculation and inoculation with Pseudomonas fluorescens and Bacillus subtilis bacteria). The activities of catalase (CAT), peroxidase (POD), and superoxide dismutase (SOD) and biochemical parameters like total chlorophyll, proline, and malondialdehyde (MDA) were quantified at the flowering stage. The results indicated that the combined treatment of 50% chemical fertilizer + biofertilizer possessed the maximum antioxidant enzyme activity; therefore, the CAT, POD, and SOD activities were increased by 42%, 35%, and 28% respectively compared to the control (p < 0.05). Moreover, the total chlorophyll content in the treatment was 1.8 mg g-1 wet weight, which was 23% superior to that in the single chemical treatment (100 kg). The levels of proline and MDA also decreased to 24.6 μmol g-1 and 3.1 nmol g-1, respectively, indicating reduction in oxidative stress. The 100% chemical fertilizer treatment with no biofertilization provided the worst performance in support of the plant defence mechanism. This study confirms the effectiveness of biofertilizers in chemical fertilizer reduction levels up to 50% and improvement of the physiological stability of cumin plants.

Keywords

References

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Caspian Journal of Environmental Sciences
Volume 23, Issue 3
July 2025
Pages 741-748

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