Development of a functional instant drink using soy isolate and collagen hydrolysate

Articles in Press

Document Type : Research Paper

Authors

1 Kazakh Research Institute of Processing and Food Industry, Almaty, Kazakhstan

2 JSC Almaty Technological University, Almaty, Kazakhstan

3 South Kazakhstan University named after M. Auezova,Shymkent, Kazakhstan

4 South Kazakhstan University named after M. Auezova, Shymkent, Kazakhstan

10.22124/cjes.2025.8857

Abstract

This study presents the development and optimization of a functional instant drink based on soy protein isolate and collagen hydrolysate. The amino acid composition analysis demonstrated the complementarity of the two protein sources: soy isolate is rich in essential amino acids crucial for muscle protein synthesis, while collagen hydrolysate contributes high levels of glycine, proline, and hydroxyproline, important for connective tissue health. A 1:1 protein blend ensured a balanced amino acid profile, enhancing both nutritional and functional properties. Using response surface methodology (RSM) and second-order regression modeling, the optimal formulation was identified to ensure maximum solubility (90.36%) and excellent organoleptic characteristics (9.25/9 points). The statistical analysis confirmed the significance of soy isolate and collagen hydrolysate levels, as well as their interactions with psyllium fiber. Notably, collagen showed a consistently positive impact on solubility and sensory properties, while psyllium negatively influenced both parameters at higher concentrations. The final formulation demonstrated high protein content (87.3%), dry matter content (96.3%), and compliance with microbiological and toxicological safety standards. These results highlight the potential of the developed instant beverage as a functional product suitable for preventive nutrition, sports applications, and the nutraceutical market.

Keywords

References

Akharume, FU, Aluko, RE & Adedeji, AA 2021, Modification of plant proteins for improved functionality: A review. Comprehensive Reviews in Food Science and Food Safety, 20: 198-224.
Andreani, G, Sogari, G, Marti, A, Froldi, F, Dagevos, H & Martini, D 2023, Plant-based meat alternatives: Technological, nutritional, environmental, market, and social challenges and opportunities. Nutrients, 15: 452.
Capita, R, Prieto, M & Alonso-Calleja, C 2004, Sampling methods for microbiological analysis of red meat and poultry carcasses. Journal of Food Protection, 67: 1303–1308.
Carey, CN, Paquette, M, Sahye-Pudaruth, S, Dadvar, A, Dinh, D, Khodabandehlou, K, Liang, F, Mishra, E, Sidhu, M & Brown, R 2023, The environmental sustainability of plant-based dietary patterns: A scoping review. The Journal of Nutrition, 53: 857-869.
Delgado-Andrade, C, Navarro, M, Lopez, H & López, MC 2003, Determination of total arsenic levels by hydride generation atomic absorption spectrometry in foods from south-east Spain: Estimation of daily dietary intake. Food Additives and Contaminants, 20: 923-932.
Gerliani, N, Hammami, R & Aider, M 2019, Production of functional beverage by using protein-carbohydrate extract obtained from soybean meal by electro-activation. LWT, 113: 108259.
Gupta, A, Sanwal, N, Bareen, MA, Barua, S, Sharma, N, Joshua Olatunji, O, Prakash Nirmal, N & Sahu, JK 2023, Trends in functional beverages: Functional ingredients, processing technologies, stability, health benefits, and consumer perspective. Food Research International (Ottawa, Ont.), 170: 113046.
Gupta, A, Sanwal, N, Bareen, MA, Barua, S, Sharma, N, Olatunji, OJ, Nirmal, NP & Sahu, JK 2023, Trends in functional beverages: Functional ingredients, processing technologies, stability, health benefits, and consumer perspective. Food Research International, vol. 170: 113046.
Intrasook, J, Tsusaka, TW & Anal, AK 2024, Trends and current food safety regulations and policies for functional foods and beverages containing botanicals. Journal of Food and Drug Analysis, 32: 112-139.
Jarvas, G, Guttman, A, Miękus, N, Bączek, T, Jeong, S, Chung, DS, Pätoprstý, V, Masár, M, Hutta, M & Datinská, V 2020, Practical sample pretreatment techniques coupled with capillary electrophoresis for real samples in complex matrices. TrAC Trends in Analytical Chemistry, 121: 115702.
Kuprina, E, Yakkola, A, Kopylov, A, Zashikhin, M & Kuznetsova, A 2020, Development of functional product enriched with collagen hydrolysate from fish processing waste. E3S Web of Conferences, 6026.
Lee, HJ, Yong, HI, Kim, M, Choi, Y-S & Jo, C 2020, Status of meat alternatives and their potential role in the future meat market—A review. Asian-Australasian Journal of Animal Sciences, 33(10):1533.
Lee, J-S, Choi, I & Han, J 2022, Construction of rice protein-based meat analogues by extruding process: Effect of substitution of soy protein with rice protein on dynamic energy, appearance, physicochemical, and textural properties of meat analogues. Food Research International, 161: 111840.
Li, P & Wu, G 2018, Roles of dietary glycine, proline, and hydroxyproline in collagen synthesis and animal growth. Amino acids, 50: 29-38.
Liu, H, Xu, X, Cui, H, Xu, J, Yuan, Z, Liu, J, Li, C, Li, J & Zhu, D 2023, Plant-based fermented beverages and key emerging processing technologies. Food Reviews International, 39: 5844-5863.
Lu, D, Roy, D, Acevedo-Fani, A, Singh, H & Ye, A 2025, Investigation of various plant protein ingredients for processed cheese analogues: physical properties and microstructure compared with milk proteins. International Journal of Food Science and Technology, 60: 018.
Mefleh, M, Pasqualone, A, Caponio, F, De Angelis, D, Natrella, G, Summo, C & Faccia, M 2022, Spreadable plant‐based cheese analogue with dry‐fractioned pea protein and inulin–olive oil emulsion‐filled gel. Journal of the Science of Food and Agriculture, 102(12): 5478-5487.
Myers, RH, Montgomery, DC & Anderson-Cook, CM 2016, Response Surface Methodology: Process and Product Optimization Using Designed Experiments, John Wiley & Sons.
Nonglait, DL, Chukkan, SM, Arya, SS, Bhat, MS & Waghmare, R 2022, Emerging non-thermal technologies for enhanced quality and safety of fruit juices. International Journal of Food Science and Technology, 57: 6368–6377.
Puiggròs, F, Muguerza, B, Arola‐Arnal, A, Aragonès, G, Suárez‐Garcia, S, Bladé, C, Arola, L & Suárez, M 2017, Functional beverages. Innovative Technologies in Beverage Processing, pp. 275-296.
Sharma, Shagun, Singh, A, Sharma, Swati, Kant, A, Sevda, S, Taherzadeh, MJ & Garlapati, VK 2021, Functional foods as a formulation ingredient in beverages: technological advancements and constraints. Bioengineered, 12: 11055-11075.
Short, EC, Kinchla, AJ & Nolden, AA 2021, Plant-based cheeses: A systematic review of sensory evaluation studies and strategies to increase consumer acceptance. Foods, 10: 725.
da Silva, VT, Mateus, N, de Freitas, V & Fernandes, A 2024, Plant-Based Meat Analogues: Exploring Proteins, Fibers and Polyphenolic Compounds as Functional Ingredients for Future Food Solutions. Foods,13(14): 1-33.
Tang, J, Yao, D, Xia, S, Cheong, L & Tu, M 2024, Recent progress in plant-based proteins: From extraction and modification methods to applications in the food industry. Food Chemistry: X, 23: 101540.
Vogelsang-O’Dwyer, M, Zannini, E & Arendt, EK 2021, Production of pulse protein ingredients and their application in plant-based milk alternatives. Trends in Food Science & Technology, 110: 364-374.
Wagner, AO, Markt, R, Mutschlechner, M, Lackner, N, Prem, EM, Praeg, N & Illmer, P 2019, Medium preparation for the cultivation of microorganisms under strictly anaerobic/anoxic conditions. Journal of Visualized Experiments: JoVE, 150: 10-3791.
Zarubin, NY, Kharenko, EN, Bredikhina, OV, Lavrukhina, EV, Rysakova, KS, Novikov, VY, Leonov, GE, Vakhrushev, IV, Zolotarev, KV & Mikhailov, AN 2024, An isotonic drink containing pacific cod (Gadus macrocephalus) processing waste collagen hydrolysate for bone and cartilage health. Marine Drugs, 22: 202.
Zhang, T, Dou, W, Zhang, X, Zhao, Y, Zhang, Y, Jiang, L & Sui, X 2021, The development history and recent updates on soy protein-based meat alternatives. Trends in Food Science & Technology, 109: 702–710.
Caspian Journal of Environmental Sciences

Articles in Press, Corrected Proof
Available Online from 26 June 2025

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