Effects of the methanolic extract of citrus limon peeling on the liver enzymes alterations in rats due to the CPF toxicity

Document Type : Research Paper

Authors

1 Professor, Head of the Department of Neurology, Faculty of Postgraduate Education, Samarkand State Medical University, Uzbekistan

2 PhD, Senior Lecturer, Department of Orthopedics and Dentistry, Bukhara State Medical Institute, Bukhara, Uzbekistan

3 3Doctor of Technical Sciences, Head of the Department of Agronomy, Navoi State University of Mining and Technologies, Navoiy, Uzbekistan

4 Associate Professor, Head of the Department of Biotechnology and Food Safety, Bukhara State University, Bukhara, Uzbekistan

5 5Department of Psychology and Medicine, Mamun University, Khiva Uzbekistan

6 Lecturer, Department of Biotechnology and Food Safety, Bukhara State University, Bukhara, Uzbekistan

7 PhD, Department of Otorhinolaryngology, Bukhara State Medical Institute, Bukhara, Uzbekistan

8 Dotsent, Department of “General and pediatric surgery”, Tashkent Medical Academy, Tashkent, Uzbekistan

9 Department of Ecology and Water Resources Management, "Tashkent Institute of Irrigation and Agricultural Mechanization Engineers" National Research University, Tashkent, Uzbekistan

10 Master of the Department of Practical Psychology, Tashkent State Pedagogical University named after Nizami, Tashkent, Uzbekistan

11 Kimyo International University in Tashkent, Shota Rustaveli Street 156, 100121, Тashkent, Uzbekistan

12 Associate Professor, Doctor of Philosophy (PhD) in Pedagogical Sciences, Kokand State University, Kokand, Uzbekistan

13 Teacher, Pharmaceuticals and Chemistry, Faculty of Medicine, Alfraganus University, Tashkent, Uzbekistan

10.22124/cjes.2025.8734

Abstract

This study evaluates the effects of the methanolic extract of Citrus limon peeling on liver enzyme changes in rats exposed to chlorpyrifos (CPF), a widely used organophosphate insecticide. Chlorpyrifos is known for its detrimental effects on non-target organisms and poses significant health risks due to its persistence in the environment and accumulation in the food chain. The research involved fifteen female Wistar rats, which were divided into three groups: a model group receiving CPF, a control group receiving PBS, and a treatment group receiving both CPF and the lemon extract. The study aimed to assess the hepatoprotective properties of the lemon peel extract, which has been previously shown to mitigate liver damage caused by various toxins. The experimental design included administering CPF intraperitoneally for four weeks, followed by oral treatment with the lemon extract. Blood samples were collected to measure serum liver enzymes, specifically serum glutamic-oxaloacetic transaminase (SGOT), serum glutamic-pyruvic transaminase (SGPT), and alkaline phosphatase (ALP), which are indicators of liver function. Results indicated that the administration of the methanolic extract of Citrus limon significantly reduced the elevated levels of serum SGOT, SGPT, and ALP in the CPF-treated rats, suggesting a protective effect against liver damage. Histopathological evaluations further supported these findings, demonstrating improvements in liver tissue integrity in the treatment group compared to the model group. This study highlights the potential of limon peel extract as a natural hepatoprotective agent, offering a promising alternative for mitigating the toxic effects of organophosphate pesticides in agricultural practices.
 

Keywords

References

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Caspian Journal of Environmental Sciences
Volume 23, Issue 4
October 2025
Pages 1051-1058

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