Protective effects of 1, 8-cineole (eucalyptol) against CCl4 induced hepatic oxidative damage in rats

Articles in Press

Document Type : Research Paper

Authors

1 Candidate of Medical Sciences, Associate Professor - Head of the Department of Public Health, Healthcare Management, Bukhara State Medical Institute, Uzbekistan

2 PhD in Veterinary Sciences, Senior Researcher at the Helminthology Laboratory, Veterinary Scientific Research Institute, Taylok region, Samarkand, Uzbekistan

3 Doctor of Chemical Sciences, Professor, Jizzakh State Pedagogical University, Jizzakh, Uzbekistan

4 Assistant, Department of "Engineering Graphics and Design Theory", "Tashkent Institute of Irrigation and Agricultural Mechanization Engineers", National Research University, Tashkent, Uzbekistan

5 Doctor of Philosophy (PhD Veterinary Science), Department of Agronomy, Navoi State University of Mining and Technologies, Navoi, Uzbekistan

6 Teacher at the Department of Neurology, Faculty of Postgraduate Education, Samarkand State Medical University, Samarkand, Uzbekistan

7 Lecturer, Department of Art Science, Karakalpak State University named after Berdakh, Nukus, Republic of Karakalpakstan

8 Senior Teacher, Department of Pharmaceuticals and Chemistry, Faculty of Medicine, Alfraganus University, Tashkent, Uzbekistan

9 Kimyo International University in Tashkent, Tashkent, Uzbekistan

10 Professor Institute of Agrobiotechnology and Food Securite of Samarkand State University named after Sharof Rashidov, Jamboy district, Samarkand, Uzbekistan

10.22124/cjes.2025.8686

Abstract

Carbon tetrachloride (CCl₄) is a hepatotoxin which induce oxidative stress. Therefore, supplementation with antioxidants may be beneficial in mitigating its hepatotoxic effects. 1,8-Cineole (1,3,3-trimethyl-2-oxabicyclo [2.2.2] octane), commonly referred to as eucalyptol has various therapeutic properties. Hence, this research was conducted to evaluate the protective effects of eucalyptol against CCl4 induced hepatic oxidative damage in rats. Thirty rats were divided randomly into the following three equal groups (n = 10): healthy control, CCl4-poisoned group (1 mL/kg BW dissolved in olive oil (1:1 volume) and eucalyptol treated group (100 mg/kg/day). After treatment period (14 days), serum sample was prepared and liver was taken out for measurement of biochemical parameters including liver enzymes such as Alanine transaminase (ALT) and Aspartate transaminase (AST) as well as oxidative stress markers including Malondialdehyde (MDA), Glutathione (GSH), Catalase (CAT), Superoxide dismutase (SOD) and Glutathione Peroxidase (GPX). The results showed that following CCl4 poisoning, a significant reduction in the levels of GSH, GPX, CAT and SOD was observed in the CCl4-poisoned group compared to the control, as well as a significant elevation in the levels of AST, ALT and MDA was observed in the CCl4-poisoned group in comparison with the control (p < 0.05). Furthermore, there was a significant increase in the levels of GSH, GPX, CAT and SOD in the eucalyptol treated group compared to the CCl4-poisoned group. However, there was a significant decrease in the levels of AST and ALT in the eucalyptol-treated group compared to the CCl4-poisoned group (p < 0.05). However, there was no significant in the MDA level in the eucalyptol-treated group compared to the CCl4-poisoned one (p > 0.05). Eucalyptol may be effective in the prevention of CCl4-induced liver damage through the reduction of oxidative stress, liver damage caused by elevated liver enzymes, and increasing antioxidant enzymes.

Keywords

References

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Caspian Journal of Environmental Sciences

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Available Online from 22 April 2025

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