Ameliorative effect of Vitamin D on CPF toxicity by evaluation of Wistar rat liver enzymes and kidney biomarkers

Articles in Press

Document Type : Research Paper

Authors

1 DSc, Associate Professor of Department of Neurology, Vice Rektor of Bukhara State Medical Institute, Bukhara, Uzbekistan

2 Kimyo International University in Tashkent Shota Rustaveli Street 156, 100121, Тashkent, Uzbekistan

3 Head of the Department of Pediatrics-2, Fergana Medical Institute of Public Health, Fergana, Uzbekistan

4 Mamun University, Khiva, Uzbekistan

5 Assistant of the Neurology Department, Faculty of Postgraduate Education, Samarkand State Medical University, Uzbekistan

6 Oncologist, Pediatric Oncosurgery, Interventional Oncologist; Scientific Researcher in the Department of "Oncology and Hematology", the National Children's Medical Center, 294 Parkent street, Tashkent, Uzbekistan

7 Director, Scientific Research Institute of Cereals and Leguminous Crops, Navoi Scientific Experimental Station, Navoiy, Uzbekistan; Scientific Researcher, Navoi State University of Mining and Technologies, Navoiy, Uzbekistan

8 Doctor of Philosophy (PhD) in Pedagogical Sciences, Associate Professor, Head of the Department of "Engineering Graphics and Design Theory" of the National Research University, "Tashkent Institute of Irrigation and Agricultural Mechanization Engineers", Tashkent, Uzbekistan

9 Professor, Pharmaceuticals and Chemistry, Faculty of Medicine, Alfraganus University, Tashkent, Uzbekistan

10.22124/cjes.2025.8571

Abstract

Chlorpyrifos (CPF) is a hepatotoxic agent that adversely affects multiple organ systems. This study investigated how Vitamin D mitigates liver and kidney damage caused by CPF exposure. Fifteen Wistar rats were divided into three groups: CPF (3 mg/kg BW), Control (PBS), and Vitamin D (1 µg/g BW). Serum total antioxidant capacity (TAC) and total oxidative stress (TOS) were measured, along with enzyme levels, to assess liver and kidney function. CPF treatment resulted in elevated biomarkers indicating organ damage, while Vitamin D treatment decreased these biomarkers and enzyme levels. The protective effects of vitamin D may stem from its antioxidant and anti-inflammatory properties. Notably, blood urea nitrogen (BUN), serum glutamic-oxaloacetic transaminase (SGOT), serum glutamic-pyruvic transaminase (SGPT), and alkaline phosphatase (ALP) levels were significantly lower in the Vitamin D group than in the CPF group. TAC levels in the treated group were lower than in controls, indicating increased oxidative stress, while TOS levels rose significantly, suggesting oxidative damage. These results highlight CPF's role in renal and hepatic injuries and emphasize the importance of enzyme analysis in assessing hepatotoxicity potentially influenced by Vitamin D. The research concludes that Vitamin D supplementation significantly mitigates liver and kidney damage caused by CPF exposure. This is evidenced by lower levels of liver enzymes and kidney biomarkers in the Vitamin D group compared to the CPF group, indicating a protective role against organ damage.

Keywords

References

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Caspian Journal of Environmental Sciences

Articles in Press, Corrected Proof
Available Online from 03 March 2025

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