Document Type : Research Paper
Authors
1
PhD, Associate Professor of the Medical Chemistry Department, Andijan State Medical Institute. Uzbekistan
2
Doctor of Philosophy in Agricultural Sciences (PhD), Senior lecturer of the Department of "Animals, Genetics, Breeding and Feeding Technology", Samarkand State University of Veterinary, Animal Husbandry and Biotechnology, Samarkand, Uzbekistan
3
DSc in Economics, Professor International School of Finance and Technology, Tashkent, Uzbekistan; Alfraganus University, Tashkent, Uzbekistan
4
PhD in Biology, Department of Agronomy, Navoi State University of Mining and Technologies, Navoi, Uzbekistan
5
Specialist of the Department of Science and Innovation, Western Caspian University, Baku, Azerbaijan
6
Kimyo international university in Tashkent Shota Rustaveli street 156, 100121, Тashkent, Uzbekistan
7
DSc, Professor of the Department of Dentistry Faculty of Postgraduate Education, Samarkand State Medical University, Samarkand, Uzbekistan
8
PhD in Veterinary Sciences, Junior Researcher, Virology Laboratory, Veterinary Scientific Research Institute, Taylok region, Samarkand, Uzbekistan
9
Independent Researcher of the Department of "Engineering Graphics and Design Theory" of the National Research University "Tashkent Institute of Irrigation and Agricultural Mechanization Engineers" Tashkent, Uzbekistan
10
Assistant of the Department of Pathological Physiology and Pathological Anatomy, Fergana Medical Institute of Public Health, Fergana, Uzbekistan
10.22124/cjes.2025.8570
Abstract
The growing recognition of the health-promoting potential of compounds derived from natural sources, particularly those exhibiting antimicrobial and antioxidant activities, has resulted in a surge of research publications dedicated to exploring the functional attributes of these target compounds for applications in functional foods and preventive healthcare. Pomegranate has been identified as a source of beneficial health effects attributed to its rich content of bioactive components, including polyphenols, tannins, and anthocyanins. This study investigates the effects of Punicalagin on the viability and proliferation of A549 cells, a human alveolar basal epithelial adenocarcinoma cell line. The cells were treated with varying concentrations of Punicalagin (10, 25, 50, 100, and 200 μg/mL) and punicalagin, with assessments conducted after a 72-hour incubation period. Cell viability was evaluated using the MTT assay, which measures metabolic activity, and results indicated a dose-dependent response to Punicalagin treatment. Specifically, lower concentrations (10 μg/mL) exhibited minimal cytotoxicity (viability: 86.04± 3.09%), while higher concentrations (200 μg/mL) significantly reduced cell viability to 51.17± 0.94%, indicating cytotoxic effects at elevated doses. Additionally, the study highlighted that even at non-cytotoxic concentrations, treated cells displayed reduced proliferative potential compared to control groups, suggesting that Punicalagin may impair cell growth. Punicalagin treatment also demonstrated a similar trend, where higher concentrations negatively impacted cell proliferation. The findings underscore the importance of concentration in determining the cytotoxic and proliferative effects of these compounds on A549 cells, providing insights into their potential therapeutic applications and mechanisms of action. Overall, this research contributes to the understanding of how natural compounds like punicalagin can influence cancer cell behavior, paving the way for future studies aimed at developing effective cancer treatments.
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