Antibacterial and anti-fungal effects of water and alcoholic extracts of Portulaca oleracea L.

Document Type : Research Paper

Authors

1 Department of Biology, Sari Branch, Islamic Azad University, Sari, Iran

2 Department of Biology, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran

10.22124/cjes.2024.8018

Abstract

Portulaca oleracea is a folk medicine in various countries. This study aimed to consider the anti-microbial effects of the water and alcoholic extracts of P. oleracea and its orange leaf extracts against Staphylococcus aureus, Streptococcus salivarius, Klebsiella, Pseudomonas aeruginosa and Candida albicans strains. Plant extracts were obtained using the maceration method. The antimicrobial activity of the extracts was measured using the Disc agar diffusion (DAD) test method. The minimum inhibitory (MIC) and bactericidal (MBC) concentrations for each extract were considered by the macro dilution method. Both aqueous and methanolic extracts showed acceptable antibacterial and anti-fungal activities at all concentrations (from 30 mg mL-1 to pure extract. However, pure extracts showed significantly higher inhibitory effects compared to the other concentrations (p < 0.01). The inhibitory effect of both aqueous and methanolic extracts was significantly increased in proportion to their concentrations (p < 0.05); however, methanolic extract was slightly more effective than aqueous extract. Compared to ampicillin antibiotics, pure methanolic and aqueous extracts showed significantly higher inhibitory effects against S. aureus and Klebsiella (p < 0.05). S. aureus was a more sensitive strain to methanolic (showing the MIC of 30 mg mL-1 and MBC of 50 mg mL-1) and aqueous (showing the MIC of 50 mg mL-1  and MBC of 70 mg mL-1). P. oleracea extracts, especially alcoholic extract, exhibited acceptable antibacterial and anti-fungal properties against various microorganisms. However, further clinical research is needed to obtain information about the effectiveness of the plant extracts such as Antibacterial, Anti-fungal, Water extract, Alcoholic extract, MIC, MBC.

Keywords

References

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Caspian Journal of Environmental Sciences

Articles in Press, Corrected Proof
Available Online from 18 September 2024

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