Ovarian histological and gene expression analyses of two Rutilus frisii (Nordmann, 1840) races during spawning migration

Articles in Press

Document Type : Research Paper

Authors

1 Department of Fisheries, Faculty of Natural Resources, University of Guilan, P.O. Box 1144, Sowmehsara, Iran

2 Fisheries Department, Faculty of Natural Resources, University of Guilan, P.O. Box 1144, Sowmehsara, Iran

3 2- Shahid Motahary Cold-water Fishes Genetic and Breeding Research Center, Iranian Fisheries Sciences Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Yasouj, Iran

10.22124/cjes.2024.8001

Abstract

Caspian kutum (Rutilus frisii) is the most common species of the Cyprinidea family in the Iranian coastal waters of the Caspian Sea and comprises, on average, more than 70% of the annual commercial bony fish catch. This species consists of two migratory forms, the spring race (SR) and autumn race (AR), which show morphological identities. The morphological differences are classified as phenotypic markers and cannot be a reliable method for distinguishing individual and populations. Therefore, we performed histological analysis concerning ovarians, as well as gene expression profiling differences, to gain more knowledge about the differential migratory behavior of the races. Ovary and liver samples were collected to observe  the  reproductive  performance  based  on  the  ovarian  histology  and  related gene  expression  using  real-time  quantitative  polymerase  chain reaction (qRT-PCR). Histological study of the AR and the SR broods showed that the ovaries were at vitellogenic (oocyte diameter is 978.4 ± 32.3 µm) and maturation (oocyte diameter is 1225 ± 27.6 µm) stages, respectively. The steps identified were predictable because the AR broods need a hibernate after migration for spawning, and the SR broods are usually ready for spawning after moving into the spawning grounds. Additionally, we performed qRT-PCR to confirm the genes involved in the vitellogenic and maturation stages of ovarian development in R. frisii. Given the lack of information about the population aspects of this species, histological and gene profiling analyses in the current study showed evidence of different migratory behaviors in the two races. These valuable results can be used in fishery management programs.

Keywords

Caspian Journal of Environmental Sciences

Articles in Press, Corrected Proof
Available Online from 07 September 2024

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