In vitro study of macro and micro nutrients decline in MS medium on growth and development levels of Acacia tortilis (Forssk.) Hayne.

Document Type : Research Paper

Authors

1 Department of Forestry, Sari University of Agricultural Science and Natural Resources, Sari, Iran

2 Department of Medicinal Plants, Faculty of Medicinal Plants, Amol University of Special Modern Technologies, Amol, Iran

10.22124/cjes.2024.7966

Abstract

Acacia tortilis is one of the valuable species growing in the southern regions of Iran, which is highly considerable for a variety of reasons such as stabilization of quicksand, medicinal uses and ecological functions. Low germination capacity of seeds, hard rooting of cuttings, the value of aesthetics and multiple medicinal uses highlight the importance of further research to explore methods for easy and efficient propagation of this species in vitro. However, seeds of A. tortilis were obtained from Khazar Center of Forest seeds located in Koloudeh, Mazandaran Province, Iran and a hot water treatment for 15 seconds was used for seed dormancy breakage. Then, seeds were cultured aseptically in MS basal medium without growth regulators. In this work, to investigate the reaction of seeds to different variables in culture medium, various concentrations of macro and micro nutrients (0, 1, 1/2, 1/4) were studied. Briefly, total treatments (T) were 10 and each treatment was replicated 3 times, with 10 members. Finally, ANOVA and Duncan tests were used to analyze data. Results showed that the best method for seed sterilization was obtained though using HgCl2 (0.5%) for 10 minutes and ethanol (70%) for one minute. In addition, results showed that the highest root and stem length, fresh and dry weight of stem and root belonged to T2 (macro elements + 1/4 micro elements), while T4 (macro elements + micro elements) was in the second order and the lowest values of the abovementioned parameters were found in T8.
 

Keywords


Ali, YH, Salma, A & Warag, E 2012, In vitro micropropagation of Acacia Senegal (L.) Willd. A multipurpose forest tree. University of Africa Journal of Sciences, 2: 58-76.
Azia, AAS, Omari, MA & Kafawin, OM 2002, Micropropagation of Acacia tortilis subsp. raddiana and A. nilotica under in vitro conditions, Journal of Tropical Forest Science, 14: 329-336.
Conesa-Sevilla, J 2011, The Tree of Everything: Acacia tortilis and the Origins of the Aesthetic Mind. Hawaiian Mythology, 157 p.
Dhabhai, K, Sharma, MM & Batra, A 2010, In vitro clonal propagation of Acacia nolitica (L.): A nitrogen fixing tree. Researcher, 2: 7-11.
Esna Ashari, M & Zokaee khosroshahi, MR 2013, Plant tissue culture. A comprehensive guide. Bu-Ali Sina University Press, 475 p.
Girijashankar, V 2011, Micropropagation of multipurpose medicinal tree Acacia auriculiformis, Journal of Medicinal Plant Research, 5: 462-466.
Golamian, F, Keshkar, S, Amiri, MS & Kazerouni, H 2015, The most suitable method for germination of rare and endangered seeds of Acacia albida to produce seedling and regenerate. 2nd Scientific Research Congress on Development and Promotion of Agricultural Sciences, Natural Resources and Environment, pp. 91-95.
Haider, A, Muhammad, Q & Beena, N 2010, Rapid in vitro multiplication of Acacia nilotica subsp. hemispherica, a critically endangered endemic taxon. Pakistan Journal of Botany, 42: 4087-4093.
Haliza, I, Sures, KM, Aziah, MY, Nor Hasnida, H & Nor Aini, AS 2016, In vitro micropropagation of Acacia auriculiformis from selected junenile sources. Dendrobiology Journal, 75: 157-165.
Hasanuzzaman, M, Araújo, S & Singh Gill, S 2020, The plant family Fabaceae, biology and physiological responses to environmental stresses, Springer Press, 547 p.
Hosseini Nasr, SM 2015. Plant cell tissue and organ culture. Aeeizh Press, 378 p.
Javed, SB, Anis, M, Khan, PR & Aref, M 2012, In vitro regeneration and multiplication for mass propagation of Acacia ehrenbergiana Hayne: a potential reclaiment of denude arid lands. Agroforest Syst. 87: 621-629.
Khalafalla, MM & Daffalla, M 2008, In vitro micropropagation and micrografting of gum Arabic tree, Acacia Senegal (L.) Willd. International Journal of Sustainable Crop Production, 3: 19-27.
Murashige, T & Skoog, F 1962, A revised medium for rapid growth and bioassays with tobacco tissue culture. Plant Physiology, 15: 473- 479.
Nabipour, A, Hosseini Nasr, SM & Hojati, SM 2015, Determination of the optimal treatment to dormancy breakage and increase germination of Acacia salicina seed. National Conference on New Ideas in Agriculture, Environment and Tourism, Ardabil, Iran, pp. 74-81.
Nndwani, D 1995, In vitro micropropagationof a tree legume adapted to arid lands Acacia tortilis subsp. raddiana, Annals of Forest Science, 52: 183-189.
Rout, GR, Senapati, SK & Aparajeta, S 2008, Micropropagation of Acacia chundra (Roxb) DC. Horticultural Science (Prague), 35: 22-26.
Saikat, G, Superabuddha, K & Prakash, KD 2016, Acacia: An exclusive survey on in vitro propagation. Journal of the Saudi Society of Agricultural Sciences, pp. 1-15.
Shahinozzaman, M, Azad, MAK & Amin, MN 2012, In vitro clonal propagation of a fast growing legume tree- Acacia mangium Willd. Employing cotyledonary node explants. Notulae Scientia Biologicae, 4: 79-85.
Tanabe, M & Honda, M 1999, Acacia koa: In vitro germination and callus culture, J. Hawaiian Pacific Agric, 10: 47-51.
Vengadesan, G, Ganapathi, A, Amutha, S & Selvaraj, N 2002, In vitro propagation of Acacia Species: A review. Plant Science, 163: 663-671.
Vengadesan, G, Ganapathi, A, Prem Anand, R & Ramesh Anbazhagan, V 2000, In vitro organogenesis and plant formation in Acacia sinuate. Plant cell, Tissue and Organ Culture, 61: 23-28.
Yadav, P, Kant, R & Kothiyal, P 2013, A review on Acacia tortilis. International Journal of Pharmaceutical and Phytopharmacological Research, 3: 93-93.
Yahia, HA 2009, Tissue culture in Sudan forest in vitro germination and micropropagation of Acacia tortilis subspp. spirocarpa, a multipurpose forest tree. Journal of Genetic engineering and Biotechnology, 7: 5-10.