Seasonal dynamics of fatty acid composition of Artemia cysts lipids from lakes of Pavlodar Region, Kazakhstan

Document Type : Research Paper

Authors

1 Laboratory of Hydrobiology, Fisheries Research and Production Centre, Almaty, Kazakhstan

2 Laboratory of Biochemistry, Al-Farabi Kazakh National University, Almaty, Kazakhstan,

3 Laboratory of Hydrobiology, Director of Aral branch Fisheries Research and Production Centre, Kyzylorda, Kazakhstan

10.22124/cjes.2024.7545

Abstract

The purpose of this research was to study the fatty acid composition of total lipids of Artemia cysts (Artemia sp.), taken from four lakes in the Pavlodar region of Kazakhstan in different seasons. Extraction of total lipids was carried out with a binary mixture of organic solvents chloroform-ethanol (2:1). Gas chromatographic separation of fatty acids was carried out after methanolysis of lipids to obtain methyl esters of fatty acids. Quantification is based on the internal standard method using a calibration curve. The seasonal dynamics in the content of both the main groups of fatty acids and individual fractions is shown since a change in ambient temperature induced a modification of the fatty acid composition of lipids. In particular, by autumn, a decrease in the synthesis of monoenoic acids and an increase in the amount of saturated fatty acids were established. Changes in the qualitative composition of saturated fatty acids were also noted, in particular, in the autumn samples, there were no such acids as C6:0 caproic, C8:0 caprylic, C10:0 capric, C11:0 undecanoic, C12:0 lauric, C13:0 tridecanoic.

Keywords


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