GC-MS analysis of Moringa oliefera root and stem branch extracts grown in Ramadi City, Iraq

Document Type : Research Paper

Authors

1 Scientific Affair, University of Anbar, Iraq

2 Department of Biology, College of Science, University of Anbar, Iraq

3 Conservation Agriculture, Centre of Desert Studies, University of Anbar, Iraq

4 Department of Animal Production, College of Agriculture, University of Anbar, Iraq

5 Department of Medical Laboratories Techniques, Al-Maarif University College, Iraq

Abstract

In the present study, phytochemical analyses were performed on the methanolic and ethanolic extracts of Moringa oliefera root and branches of the stem using Gas Chromatography-Mass spectrometry (GC-MS). This plant has therapeutic properties in its different parts. GC-MS analysis revealed the presence of many phytochemicals in root methanolic extracts involving 14 active compounds. It was found that a high percent area is 2-furancarboxylaldehyde (50.07%). Ethyl aldehyde had the lowest area (0.49%). In contrast, ethanolic extract of root contained 19 active compounds as GC-MS isolated and characterized. The technique confirmed that 6-octadecenoic acid was the highest compound (59.43%), while N-sulfinyl butylamine has rarely been detected in plant extracts (0.12%). Many other phytochemicals were also detected. Similarly, methanol extract for stem branches included three active compounds. A 5-hydroxymethylfufural was the most abundant (63.06%), while 2-acetyl-2H-tetrazole possessed the lowest area (9.70%). Ethanol extract contained six isolated active compounds. Few major compounds were found with a high percent peak area in branches by ethanol extract including 5-hydroxymethylfurfural (55.81%), whereas, the lowest one was hydrazinecarboxaldehyde (0.49%).

Keywords


Abdulateef, SM, Atalla1, OK, MQAL-Ani, Mohammed, THT, Abdulateef, FM & Abdulmajeed, OM 2021, Impact of the electric shock on the embryonic development and physiological traits in chicks embryo. Indian Journal of Animal Sciences, 90: 1541-1545.
Abdellatef, E, Nassrallah, HMDAA, Aboul-Enein, KM, El-Shemy, HA, & Khalafalla, MM 2010, Antiproliferative action of Moringa oleifera Lam. root extracts in acute myeloid leukaemia (AML) cell line. Journal of Experimental Sciences, 1(8).‏
Afzal, A, Hussain, T & Hameed, A 2021, Moringa oleifera supplementation improves antioxidant status and biochemical indices by attenuating early pregnancy stress in Beetal goats. Frontiers in Nutrition, 8. DOI: 10.3389/fnut.2021.700957
Al-Hadidy, AAA & Mostafa, SO 2022, Hormonal and histological study on the protective effect of Moringa oleifera against chromium toxicity. Caspian Journal of Environmental Sciences, 20: 477-489.
AL-Obaidi, FJ, Thaker, AA & Ramizy, A 2021, GC-Mass spectrometry analysis of Iraqi Moringa oleifera seeds extract. Indian Journal of Forensic Medicine & Toxicology, 15:  2275.‏
Al-Obaidi, FJ, Ramizy, A, & Thaker, AA 2021, Effects of Moringa oleifera seed extract on PbNP-toxicity induced in rats. In: Journal of Physics: Conference Series (Vol. 1879, No. 2, p. 022001), IOP Publishing.‏
Amaglo, NK, Bennett, RN, Curto, RBL, Rosa, EA, Turco, VL, Giuffrida, A & Timpo, G M 2010, Profiling selected phytochemicals and nutrients in different tissues of the multipurpose tree Moringa oleifera L., grown in Ghana. Food Chemistry, 122: 1047-1054.‏
Ameh, SS, & Alafi, OF 2018, Effect of ethanol extract of Moringa oleifera leaves in protecting anaemia induced in rats by aluminium chloride. IOSR Journal of Biotechnology and Biochemistry, 4: 34-52.
Asraa, AS, Thaker, AA & Hussain, A 2019, Protective activity of Capparis spinosa against aflatoxin b1 poisons induced histopathological changes in mice. Biochemical and Cellular Archives, 19: 717.‏
Atawodi, S E, Atawodi, J C, Idakwo, G A, Pfundstein, B, Haubner, R, Wurtele, G & Owen, RW 2010, Evaluation of the polyphenol content and antioxidant properties of methanol extracts of the leaves, stem, and root barks of Moringa oleifera Lam. Journal of Medicinal Food, 13: 710-716.‏
Bamola, N, Verma, P, & Negi, C 2018, A review on some traditional medicinal plants. International Journal of Life-Sciences Scientific Research, 4: 1550-1556.‏
Dzuvor, C K, Pan, S, Amanze, C, Amuzu, P, Asakiya, C, & Kubi, F 2021 Bioactive components from Moringa oleifera seeds: production, functionalities and applications: A critical review. Critical Reviews in Biotechnology, 1-23.‏
Faizi, S, Sumbul, S, Versiani, M A, Saleem, R, Sana, A, & Siddiqui, H 2014 GC/GCMS analysis of the petroleum ether and dichloromethane extracts of Moringa oleifera roots. Asian Pacific Journal of Tropical Biomedicine, 4(8), 650-654.‏
Foidl, N, Makkar, HPS & Becker, K 2001 The potential of Moringa oleifera for agricultural and industrial uses. What development potential for Moringa products, Dar Es Salaam, 20 p,‏ https://moringatrees.org/moringa-doc/the_potential_of_moringa_oleifera_for_agricultural_and_industrial_uses.pdf
Gu, R, Wang, Y, Long, B, Kennelly, E, Wu, S, Liu, B & Long, C 2014, Prospecting for bioactive constituents from traditional medicinal plants through ethnobotanical approaches. Biological and Pharmaceutical Bulletin, 37: 903-915.
Iriani, F, Rakhmiati, R, Kertasari, VD, Handayani, EP, Zuyasna, Z, W. Yanuastri, P 2023, Secondary metabolite compounds of Moringa oleifera leaves in two different urban altitude locations, Indonesia. Caspian Journal of Environmental Sciences, 21: 1065-1071.
Islam, Z, Islam, S M, Hossen, F, Mahtab-ul-Islam, K, Hasan, M, & Karim, R 2021, Moringa oleifera is a prominent source of nutrients with potential health benefits. International Journal of Food Science, https://doi.org/10.1155/2021/6627265.
Kasolo, JN, Bimenya, GS, Ojok, L, Ochieng, J & Ogwal-Okeng, JW 2010, Phytochemicals and uses of Moringa oleifera leaves in Ugandan rural communities. Journal of Medicinal Plants Research, 4(9): 753-757.‏
Manaheji, H, Jafari, S, Zaringhalam, J, Rezazadeh, S, & Taghizadfarid, R 2011 Analgesic effects of methanolic extracts of the leaf or root of Moringa oleifera on complete Freund's adjuvant-induced arthritis in rats. Zhong xi yi jie he xue bao= Journal of Chinese Integrative Medicine, 9: 216-222.‏
Mbikay, M 2012, Therapeutic potential of Moringa oleifera leaves in chronic hyperglycemia and dyslipidemia: A review. Frontiers in Pharmacology, 3: 24.‏
Muangnoi, C, Chingsuwanrote, P, Praengamthanachoti, P, Svasti, S & Tuntipopipat, S 2012, Moringa oleifera pod inhibits inflammatory mediator production by lipopolysaccharide-stimulated RAW 264.7 murine macrophage cell lines. Inflammation, 35: 445-455.
Oldoni, T L C, Merlin, N, Bicas, T C, Prasniewski, A, Carpes, S T, Ascari, J & Thomé, G 2021 Antihyperglycemic activity of crude extract and isolation of phenolic compounds with antioxidant activity from Moringa oleifera Lam. leaves grown in Southern Brazil. Food Research International, 141: 110082.‏
Padayachee, B, & Baijnath, H 2012, An overview of the medicinal importance of Moringaceae. Journal of Medicinal Plants Research, 6(48): 5831-5839,‏ DOI: 10.5897/JMPR12.1187, Available online at http://www.academicjournals.org/JMPR
Paul, CW & Didia, BC 2012, The effect of methanolic extract of Moringa oleifera lam roots on the histology of kidney and liver of guinea pigs. Asian Journal of Medical Sciences, 4: 55-60.‏
Saini, R K, Shetty, N P, Prakash, M, & Giridhar, P 2014, Effect of dehydration methods on retention of carotenoids, tocopherols, ascorbic acid and antioxidant activity in Moringa oleifera leaves and preparation of a RTE product. Journal of Food Science and Technology, 51: 2176-2182.‏
Saini, RK, Sivanesan, I & Keum, YS 2016, Phytochemicals of Moringa oleifera: A review of their nutritional, therapeutic and industrial significance. 3 Biotech, 6: 1-14. DOI: 10.1007/s13205-016-0526-3
Saleh, M M, Qader, S W, & Thaker, A A 2016 Gastroprotective activity of Eruca sativa leaf extract on ethanol-induced gastric mucosal injury in Rattus norvegicusJordan Journal of Biological Sciences, 147(3384), 1-6.‏
Salmerón-Manzano, E, Garrido-Cardenas, J A, & Manzano-Agugliaro, F 2020, Worldwide research trends on medicinal plants. International Journal of Environmental Research and Public Health, 17(10): 3376.‏
Sharma, V, Paliwal, R, Sharma, P, & Sharma, S 2011 Phytochemical analysis and evaluation of antioxidant activities of hydro-ethanolic extracts of Moringa oleifera Lam. pods. Journal of Pharmacy Research, 4: 554-557.‏
Vats, S & Gupta, T 2017, Evaluation of bioactive compounds and antioxidant potential of hydroethanolic extract of Moringa oleifera Lam. from Rajasthan, India. Physiology and molecular biology of plants : An International Journal of Functional Plant Biology, 23: 239-248, https://doi.org/10.1007/s12298-016-0407-6.