Molecular genetic analysis of the ICE1 gene in Arum korolkowii Regel

Document Type : Research Paper

Authors

1 Department of Biotechnology, Higher School of Chemical Engineering and Biotechnology, M. Auezov South-Kazakhstan University, Tauke-Khan, Shymkent, 160000, Kazakhstan

2 International Kazakh-Turkish University named after H.A.Yasavi, Turkestan city

3 Institute of Genetics and Plants Experimental Biology, Academy of Sciences of Uzbekistan, Tashkent Region, Kibray District, Yuqori-Yuz, 111226 Uzbekistan

4 South Clinical and Genetic Laboratory, South Kazakhstan Medical Academy, Shymkent, 160000, Kazakhstan

10.22124/cjes.2023.7402

Abstract

The paper presents a molecular genetic analysis of the expression of the ICE 1 gene Arum korolkowii Regel involved in the abiotic stress response in the Korolkov Aronnik in cold and frost conditions. The classical PCR method was used to identify the ICE 1 gene in Aronnik Korolkov and sequenced this site to determine the uniqueness of this gene. When stress is induced, increased expression of the studied gene is shown. It has been proved that the expression of this gene in Aronnik Korolkov begins already at the stage of acclimatization.

Keywords


Altschul, SF, Gish, W, Miller, W, Myers, EW & Lipman, DJ 1990, Basic local alignment search tool. Journal of Molecular Biology, 215: 403-410, DOI: 10.1016/S0022-2836(05)80360-2. PMID: 2231712.
Flint, J & Kendler, KS 2014, The genetics of major depression. Neuron, 81: 484-503. DOI: 10.1016/j. neuron.2014.01.027, PMID: 24507187; PMCID: PMC3919201.
Hall, TA 1999, BioEdit: A user-friendly biological sequence alignment editor and analysis program for windows 95/98/nt. Nucleic Acids Symposium Series, 41: 95-98.
Jabborova, D, Ziyadullaeva, N, Enakiev, Y, Narimanov, A, Dave, A, Sulaymanov, K, Jabbarov, Z, Singh, S & Datta, R 2023, Growth of spinach as influenced by biochar and Bacillus endophyticus IGPEB 33 in drought condition. Pakistan Journal of Botany, 55, DOI: http://dx.doi.org/10.30848/PJB2023-SI(6)
Jabborova, DP, Narimanov, AA, Enakiev, YI & Kakhramon, DD 2020, Effect of Bacillus subtilis 1 strain on the growth and development of wheat (Triticum aestivum L.) under saline condition. Bulgarian Journal of Agricultural Science, 26: 744-747.
Kanehisa, M, Goto, S, Kawashima, S & Nakaya, A 2002, The KEGG databases at GenomeNet. Nucleic Acids Research, 30: 42-46. DOI: 10.1093/nar/30.1.42. PMID: 11752249; PMCID: PMC99091.
Magrane, M 2011, UniProt Consortium. UniProt Knowledgebase: a hub of integrated protein data. Database (Oxford), 2011: bar009, DOI: 10.1093/database/bar009. PMID: 21447597; PMCID: PMC3070428.
Narimonov, A, Azimov, A, Yakubjanova, N & Shavkiev, J 2023, Scientific basis of cotton seed germination in the Central Region of Uzbekistan. SABRAO Journal of Genetics and Breeding, 55: 1561-1572, http://doi.org/10.54910/sabrao2023.55.5.10.
Nomura, T, Seto, Y & Kyozuka, J 2023, Unveiling the complexity of strigolactones: Exploring structural diversity, biosynthesis pathways and signalling mechanisms. Journal of Experimental Botany, erad412, https://doi.org/10.1093/jxb/erad412
Okonechnikov, K, Golosova, O & Fursov, M 2012, UGENE team. Unipro UGENE: a unified bioinformatics toolkit. Bioinformatics, 28:1166-7. DOI: 10.1093/bioinformatics/bts091, Epub 2012 Feb 24. PMID: 22368248.
Pagni, M, Ioannidis, V, Cerutti, L, Zahn Zabal, M, Jongeneel, CV, Hau, J, et al. 2007, MyHits: Improvements to an interactive resource for analysing protein sequences. Nucleic Acids Research, 35: W433-W437. https://doi.org/10.1093/nar/gkm352
Rogers, S, Wells, R & Rechsteiner, M 1986, Amino acid sequences common to rapidly degraded proteins: the PEST hypothesis. Science, 234: 364-368, DOI: 10.1126/science.2876518. PMID: 2876518.
Rozas, J, Sánchez DelBarrio, JC, Messeguer, X & Rozas, R 2003, DnaSP, DNA polymorphism analyses by the coalescent and other methods. Bioinformatics, 19:2496-2497, DOI: 10.1093/bioinformatics/btg359. PMID: 14668244.
Saitou, N & Nei, M 1987, The neighbour-joining method: a new method for reconstructing phylogenetic trees. Molecular Biology and Evolution,4: 406-425, DOI: 10.1093/oxfordjournals.molbev.a040454, PMID: 3447015.
Tamura, K, Peterson, D, Peterson, N, Stecher, G, Nei, M & Kumar, S 2011, MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Molecular Biology and Evolution, 28: 2731-2739. DOI: 10.1093/molbev/msr121, Epub 2011 May 4. PMID: 21546353; PMCID: PMC3203626.
Thompson, JD, Higgins, DG & Gibson, TJ 1994, CLUSTAL W: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Research, 22:4673-4680. DOI: 10.1093/nar/22.22.4673, PMID: 7984417; PMCID: PMC308517.
Tian, Y, Shuai, Y, Shao, C, Wu, H, Fan, L, Li, Y, Chen, X, Narimanov, A, Usmanov, R & Baboeva, S 2023, Extraction of cotton information with optimized phenology-based features from sentinel-2 images. Remote Sensing, 15: 1988
Vinogradov, VA 2009, Information and global problems of our time. Issues, Philosophy, 308 p.
Vorontsova, AI & Kharitonov, NE, 2005, Nature conservation, Moscow, Russia, Higher School, 216 p.
Wong, P, Skoe, E, Russo, N, et al. 2007, Musical experience shapes human brainstem encoding of linguistic pitch patterns. Nature Neuroscience, 10: 420-422, https://doi.org/10.1038/nn1872
Yeginbay, A,  Burabaev, A, Mаmykоvа, Burabaev, A, Azhibayeva Kupenova, D,  Yelemanova, Z, Esimova, A & Narymbayeva,  Z 2022,  Ways and solutions to preserve the gene pool of rare and endangered plants. Caspian Journal of Environmental Sciences, 20: 1135-1142.
Yeginbay, A, Burabaev, A, Burabaev, A, Ibraimova, Z, Yelemanova, Z, & Almira, S 2023, Study of the resistance of Arum korolkowii to high-temperature impacts of different intensity at physiological, biochemical, molecular and genetic levels. Indian Journal of Genetics and Plant Breeding, 83: 127-134.
Yeginbay, AM, Burabaev, AA, Natyrov, AK 2022, Cultivation of Arum in Low-Volume Hydroponics. Prirodnye sistemy i resursy (Natural Systems and Resources), 12: 13-19. DOI: https://doi.org/10.15688/nsr. jvolsu. 2022.3.2.