Determination of the antimicrobial effectiveness of the essential oil of Origanum vulgare against strains of Escherichia coli isolated from bovine mastitis

Document Type : Research Paper

Authors

1 Universidad Estatal de Bolivar, Facultad de Ciencias Agropecuarias, Direccion de Investigaciòn y Vinculacion, Carrera de Medicina Veterinaria, CP: 020150, Guaranda, Ecuador

2 Clínica Veterinaria Mi Amigo Fiel, CP: 060104, Riobamba, Ecuador

10.22124/cjes.2023.7202

Abstract

Milk production is mainly affected by pathologies such as mastitis, caused mainly by bacteria, where one of the most important is Escherichia coli. In this sense, the objective of the present investigation was to determine the antimicrobial effectiveness of the essential oil of Origanum vulgare against strains of E. coli isolated from bovine mastitis. Therefore, the presence of the bacterium was analyzed in the farm "La felicidad" located in the province of Chimborazo (Ecuador). The initial identification of the microorganism was made using the CMT diagnostic test. Subsequently, the isolation by means of plate culture and its characterization using the IMVIC tests were carried out. On the other hand, the essential oil of oregano was obtained by steam distillation. These oils were studied for their antimicrobial affectivity against the isolated strains of E. coli, and their MIC was also analyzed. As results, the total prevalence of mastitis was 26.25%. Of the 28 samples analyzed, 18 isolates (64.29%) corresponded to Escherichia coli. After the antimicrobial activity, oregano oil with a 60% concentration was the one that showed the best performance against the pathogen. In the CMI, from the concentration of 3% of essential oil there was an inhibitory effect. Finally, gas chromatography of oregano oil showed that formic acid and p-Cymeno were the most present. In conclusion, natural organ oil can be a phytopharmacological alternative against pathogens that cause bovine mastitis.
 

Keywords

References

 
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Caspian Journal of Environmental Sciences

Articles in Press, Corrected Proof
Available Online from 22 October 2023

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