Effect of seed protectants on fungal disease pathogens when using different technologies of oilseed flax, Linum usitatissimum cultivation

Document Type : Research Paper


A.I. Barayev Scientific and Production Center of Grain Farming, Republic of Kazakhstan



The study was carried out in 2021-2022 in the dry steppe zone of Northern Kazakhstan. The object of the study was the Kustanaysky yantar oilseed flax variety. Flax is a promising crop for this zone and requires an in-depth study of the diseases affecting it. The study aimed to evaluate the effect of Seedspor W and Olymp SC protectants on the seed infection pathogens, Fusarium ssp. and Colletotrichum ssp. against the background of different technologies of flax cultivation. Studies showed the high effectiveness of the complex use of protectants, where the degree of seed contamination decreased by two to three times compared to the control variant. By this variant, the authors obtained the lowest indicator of the degree of seed contamination (2-6%), high laboratory germination (90-95%), germination energy (79-89%), and field germination (70-87%). When restraining and suppressing the spread and development of root rot of Fusarium ssp. and Colletotrichum ssp., Seedsport W bioprotectant was inferior in efficiency to the Olymp SC chemical protectant. During the plant vegetation period, the prevalence and development of Fusarium wilt and anthracnose were more intense with no tillage, while less intense by traditional tillage. Over the years of the study, high yields were obtained in the variant with the integrated use of protectants (Olymp SC + Seedspor W + Akiba WSC) and averaged 7.1 c/ha, which was 3.9 c/ha more than the control variant.


Al-Enezi, AMA & Jamil, DS 2023, Phenotypic and molecular diagnosis of Fusarium oxysporum and Macrophomina phaseolina isolated from cucumis melon roots. Caspian Journal of Environmental Sciences, 21: 75-84.
Al-Masoodi, IH, Al-Rubaye, AFM, Hussein, HJ 2023, Isolation and diagnosis of the fungi associated with maize seeds collected from local markets in Karbala, Iraq. Caspian Journal of Environmental Sciences, 21: 665-672.
Altaf, M, Sarwar, S, Iqbal, J, Rahman, S, Mustafa, HS, Nazir, S, Habib, I & Nawaz, M 2023, Revealing the yield and quality responses of soybean advanced lines under semi-arid conditions. Advancements in Life Sciences, 10: 54-60.
Amini, S, Hassanpour Asil, M, Olfati, J, Mousanejad, S 2023, Effects of interaction between ectomycorrhizal fungal and mycorrhiza helper bacteria on Picea abies seedlings growth. Caspian Journal of Environmental Sciences, 21: 737-747.
Arinov, KK, Musynov, KM, Shestakova, NA, Serekpaev, NA & Apushev, AT 2016, Rastenievodstvo (Plant breeding). Foliant, Astana, 583 p. [In Russian].
Bacelis, K & Gruzdevien─Ś E 2001, Resistance of fiber flax varieties to Colletotrichum lini Manns et Bolley. Biologija, 47: 4-7.
Bacelis, K & Gruzdeviene, E 2003, Disease resistant variety – a way of the ecological flax protection in Lithuania. In: 4th International scientific and practical conference on environment technology. Rezekne Academy of Technologies, Rezekne, pp. 26-28, http://dx.doi.org/10.17770/etr2003vol1.1974
Dospekhov, BA 1985, Methodology of field experience (with the basics of statistical processing of research results) (5th ed.). Agropromizdat, Moscow, 351 p. 
Drakhovskaya, MD 1962, Prognoz v zashchite rastenii (Prognosis in plant protection). Selkhozliteratura, Moscow, pp. 168-173, [In Russian].
Fedin, MA, editor 1983, Metodika gosudarstvennogo sortoispytaniya selskokhozyaistvennykh kultur (Methodology for state variety testing of agricultural crops). Issue 3. Kolos, Moscow, 184 p. (In Russian).
Gosstandart of Russia 1995, GOST 12044-93. Semena selskokhozyaistvennykh kultur. Metody opredeleniya zarazhennosti boleznyami (Crop seeds. Methods for determining disease infestation). Izdatelstvo standartov, Moscow, pp. 158-159, [In Russian].
Gosstandart of USSR 1986, GOST 12038-84. Semena selskokhozyaistvennykh kultur. Metody opredeleniya vskhozhesti (Crop seeds, germination determination methods). Izdatelstvo standartov, Moscow, pp. 33-37, [In Russian].
Guarnaccia, V, Martino, I, Gilardi, G, Garibaldi, A & Gullino, ML 2021, Colletotrichum spp. causing anthracnose on ornamental plants in northern Italy. Journal of Plant Pathology, 103: 127-137. https://doi.org/10.1007/s42161-020-00684-2
Haselow, L, Rupp, H, Meißner, R & Akshalov, K 2020, Forschungsarbeiten zum Bodenwasserhaushalt in der kasachischen Steppe (Research study on the soil water balance in the steppe of Kazakhstan). WasserWirtschaftthis 110: 34-40. http://dx.doi.org/10.1007/s35147-020-0366-2 (In Russian).
Kanapin, A, Samsonova, A, Rozhmina, T, Bankin, M, Logachev, A & Samsonova, M 2020, The genome sequence of five highly pathogenic isolates of Fusarium oxysporum f. sp. lini. Molecular Plant-Microbe Interactions, 33: 1112-1115. https://doi.org/10.1094/MPMI-05-20-0130-SC
Kantarbayeva, EE, Shayakhmetova, AS, Koshen, BM & Zholamanov, KK 2017, The density of planting and the productivity of corn in the context of forest-steppe zone of Northern Kazakhstan. Asian Journal of Microbiology, Biotechnology & Environmental Sciences, 191: 116-120.
Karabanov, E 2021, Vyzovy i osobennosti 2021/22 gg v klyuchevykh segmentakh maslichnogo rynka RK (APK - Inform: Itogi No. 11(89) (Challenges and features of 2021/22 in key segments of the oilseed market of Kazakhstan (APK-Inform: Results No. 11(89). Available: https://www.apk-inform.com/ru/exclusive/opinion/1523836.
Kondratenko, EP, Soboleva, OM, Verbitskaya, NV & Filipovich, LA 2022, Evaluation of the effectiveness of the combined use of humic fertilizer and herbicides in spring wheat crops. Journal of Agriculture and Crops, 9: 130-136.
Kyzdarbekova, G, Khussainov, A, Yessenzholov, B, Memeshov, S, Durmekbayeva, S, Ayapbergenova, A & Dankina, G 2023, Effectiveness of the use of ash and slag fertilizer on ordinary chernozem for Linum usitatissimum crops. On-Line Journal of Biological Sciences, 23: 276-285. https://doi.org/10.3844/ojbsci.2023.276.285.
Lukomets, VM, Piven, VT, Tishkov, NM & Zakharova, LM 2013, Len maslichnyi – kultura perspektivnaya (Oilseed flax: a promising crop). Zashchita i karantin rastenii, S2: 61-80, [In Russian].
Mozhaev, NI, Arinov, KK, Shestakova, NA, Iskakov, MA & Serikpaev, NA 2014, Praktikum po rastenievodstvu (A practical course in plant breeding). Saken Seifullin Kazakh Agrotechnical University Astana, 309 p. [In Russian].
Mukhomedyarova, AS, Kushenbekova, AK, Elekesheva, MM, Gumarova, ZhM & Bulekova, AA 2023, Influence of nitrogen mineral fertilizer application methods on the preservation and yield of winter wheat (Triticum aestivum). Research on Crops, 24: 241-249.
Mussynov, KM, Suleimenova, ZS, Bekenova, SS, Utelbayev, YA, Bazarbayev, BB, Yessenbekova, GT & Sagatbek, SD 2019, Diseases of flax (Linum usitatissimum) and substantiation of protective measures in the conditions of the dry steppe zone of Northern Kazakhstan. Annals of Agri-bio Research, 24: 82-87.
Nasiyev, B & Dukeyeva, A 2023, Influence of mineral fertilizers and methods of basic tillage on the yield and oil content of sunflower. On-Line Journal of Biological Sciences, 23: 296-306. https://doi.org/10.3844/ojbsci.2023.296.306
Paul, S, Bhateria, S & Kumari, A 2015, Genetic variability and interrelationships of seed yield and yield components in linseed (Linum usitatissimum L.). SABRAO Journal of Breeding and Genetics, 47: 375-383.
Piven, VT, Semerenko, SA, Serdyuk, OA & Medvedeva, NV 2011, Zashchita posevov lna maslichnogo ot boleznei i vreditelei v usloviyakh Yuzhnogo Federalnogo okruga RF (Protection of oil flax crops from diseases and pests in the Southern Federal District of the Russian Federation). Maslichnye kultury. Nauchno-tekhnicheskii byulleten Vserossiiskogo nauchno-issledovatelskogo instituta maslichnykh kultur, 1: 146-147. [In Russian].
Ponomarev, AV, Kremneva, OYu, Gasiyan, KE & Danilov, RYu 2022, Vliyanie sposobov obrabotki pochvy na razvitie boleznei pshenitsy (Influence of tillage methods on the development of wheat diseases). Yug Rossii: ekologiya, razvitie, 17: 174-181. http://dx.doi.org/10.18470/1992-1098-2022-4-174-181 (In Russian).
Programma paketa prikladnoi statistiki 2004, SNEDECOR: 1-faktornyi dispersionnyi analiz ANOVA. Versiya 4.7, 05.07.2004 g [SNEDECOR Applied Statistics Package Software: One-way analysis of variance (ANOVA). Version 4.7, 05.07.2004].
Rashid, KY 2003, Principal diseases of flax. In: Muir AD, Westcott ND, editors. Flax the genus Linum. CRC Press Inc., Boca Raton, pp. 92-123.
Samsonova, A, Kanapin, A, Bankin, M, Logachev, A, Gretsova, M, Rozhmina, T & Samsonova, M 2021, A genomic blueprint of flax fungal parasite Fusarium oxysporum f. sp. lini. International Journal of Molecular Sciences, 22: 2665, https://doi.org/10.3390/ijms22052665.
Sheng, QC, Stybayev, G, Fu, WY, Begalina, A, Hua, LS, Baitelenova, A, Yuan, G, Arystangulov, S, Hua, KQ, Kipshakpayeva, G, Lin, ZX & Tussipkan, D 2022, Flax varieties experimental report in Kazakhstan in 2019. Journal of Natural Fibers, 19: 2356-2365, http://dx.doi.org/10.1080/15440478.2020.1813674.
Suleimenov, MKaskarbayev, ZAkshalov, KTulegenov, A 2016, Principles of conservation agriculture in continental steppe regions. In: L, Mueller, A, Sheudshen, F, Eulenstein (editors). Novel methods for monitoring and managing land and water resources in Siberia. Springer, Cham, pp. 667-679. http://dx.doi.org/10.1007/978-3-319-24409-9_30.
Suleimenova, AK 2018, Stability of varieties of oil floor to Fusariosis. International Journal of Agriculture And Biology, 4: 41-43, https://doi.org/10.24411/2587-6740-2018-14060
Toropova, EYu, Chulkina, VA & Stetsov, GYa 2010, Vliyanie sposobov obrabotki pochvy na fitosanitarnoe sostoyanie posevov (Influence of soil tillage methods on the phytosanitary state of crops). Zashchita i Karantin Rastenii, 1: 26-27, [In Russian].
Yadav, B, Kaur, V, Narayan, OP, Yadav, SK, Kumar, A & Wankhede, DP 2022, Integrated omics approaches for flax improvement under abiotic and biotic stress: current status and future prospects. Frontiers in Plant Science, 13: 931275. https://doi.org/10.3389/fpls.2022.931275.
Yesmagulova, BZ, Assetova, AY, Tassanova, ZB, Zhildikbaeva, AN & Molzhigitova, DK 2023, Determination of the degradation degree of pasture lands in the West Kazakhstan region based on monitoring using geo-information technologies. Journal of Ecological Engineering, 24: 179-187.