Endophytic ability of Bacillus mojavensis Ps17 to colonize various agronomic crop varieties

Document Type : Research Paper

Authors

1 Department of Agricultural Sciences, Kazan Federal University, Kazan, Russian Federation; Department of Geography and Cartography of the Institute of Management, Economics and Finance

2 Department of Geography and Cartography, Institute of Management, Economics and Finance, Kazan Federal University, Kazan, Russian Federation

3 Laboratory of Chemical-Biological Research, Arbuzov IOPC FRC Kazan Scientific Centre, Russian Academy of Sciences, Kazan, Russian Federation4. Center for Agroecological Research, Kazan State Agrarian University, Kazan, Russian Federation

4 Center for Agroecological Research, Kazan State Agrarian University, Kazan, Russian Federation

10.22124/cjes.2023.7125

Abstract

Using endophytic microorganisms as biological agents is gaining particular interest due to their multiple properties. In this study, we evaluated the ability of the endophytic bacterium, Bacillus mojavensis PS17 (Russian patent RU2737208C1) isolated from surface-sterilized wheat seeds, to colonize different agronomic crops. The plant colonization ability was studied in pot experiments containing a mixture (1:1) of the garden and bulk soil moistened with distilled water to 60% of its water-holding capacity. The bacterium culture was applied on non-sterile seed as vegetative cells diluted in PBS buffer to an optical density of 0.1 at 595 nm. As a marker, a spontaneous rifampicin-mutant (15 μg mL-1) of the studied strain was used. The plants were grown in a laboratory condition. After 3 weeks, their roots and shoot were surface-sterilized and plated onto LB medium agar amended with Rifampicin and nystatin. For the realities of results, bacterium grown on a selective medium was compared with their parent strain using fingerprinting BOX-PCR. The obtained results demonstrated that B. mojavensis PS17 can reside in tissues of all plants tested without causing any harm to plant growth and development, making it a good candidate for using as an endophyte biological agent for plant protection.

Keywords

References

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Caspian Journal of Environmental Sciences

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Available Online from 30 September 2023

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