Screening and evaluation of potential microbial bio-activators used in sewage sludge composting

Document Type : Research Paper


NCJSC “S.Seifullin Kazakh AgroTechnical Research University”, Zhenis Avenue 62, 010010 Astana City, Republic of Kazakhstan



Forty pure microbial cultures have been isolated from sewage sludge to develop biopreparations using the microbiological screening method. Among all studied microorganisms sixteen strains of different taxonomic groups (2 strains of each Bacillus and Streptomyces, 7 strains of Pseudomonas, and 1 strain of each of the following Acinetobacter, Rhizobium, Pseudarthrobacter, Sphingomonas, and Rothia) were selected providing the best cellulose-degrading ability, as well as having good growth and quick biomass accumulation on various substrates. The isolates were additionally tested for the ability to consume organic and mineral nitrogen species, and to encourage seedlings’ growth. Moreover, we have evaluated the nitrogenase activity for some strains. As a result, the following strains of Bacillus megaterium № 10, Rhizobium pusense № 25, Sphingomonas paucimobilis № 49, Pseudomonas fluorescens № 83, Streptomyces albidoflavus № 81, Streptomyces graminearus № 61 demonstrated the potential to be used as bio-activator for sewage sludge composting and as multifunctional biologics in crop production.


Altschul, SF, Madden, TL, Schäffer, AA, Zhang, J, Zhang, Z, Miller, W & Lipman, DJ 1997, Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Research, 25: 3389-3402.  DOI: 10.1093/nar/25.17.3389
Athar, T, Pandey, A, Khan, MK, Saqib, ZA, Jabeen, M, Shahid, S, Hamurcu, M, Gezgin, S, Rajput, VD & Elinson, МA 2022, Potential role of beneficial microbes for sustainable treatment of sewage sludge and wastewater. Sustainable Management and Utilization of Sewage Sludge, pp. 71-96, DOI: 10.1007/978-3-030-85226-9_4
Bansal, H, Singla, RK, Behzad, S, Chopra, H, Grewal, AS & Shen, B 2021, Unleashing the potential of microbial natural products in drug discovery: focusing on streptomyces as antimicrobials goldmine. Current Topics in Medicinal Chemistry, 21: 2374-2396, DOI: 10.2174/1568026621666210916170110.
Buhari, J, Hasan, HA, Rahim, NFM, Kurniawan, SB, Abdullah, SRS & Othman, AR 2022, Unveiling the optimal ammonia-oxidising bacterial consortium for polishing low ammonia-contaminated wastewater. Journal of Water Process Engineering, 47: 102753. DOI: 10.1016/j.jwpe.2022.102753
Capone, DG 2018, Determination of nitrogenase activity in aquatic samples using the acetylene reduction procedure. In: Handbook of methods in aquatic microbial ecology. CRC Press, pp. 621-631.
Fan, J, Chen, Y, Zhang, TC, Ji, B & Cao, L 2020, Performance of Chlorella sorokiniana-activated sludge consortium treating wastewater under light-limited heterotrophic condition. Chemical Engineering Journal, 382: 122799 DOI: 10.1016/j.cej.2019.122799
Goel, N, Patra, R, Verma, SK & Sharma, PC 2019, Purification and characterization of cellulase from Pseudomonas sp. isolated from waste dumping site soil. Journal of Applied Biotechnology and Bioengineering, 6: 118-124. DOI: 10.15406/jabb.2019.06.00183
Gupta, P, Samant, K & Sahu, A 2012, Isolation of cellulose-degrading bacteria and determination of their cellulolytic potential. International Journal of Microbiology. DOI: 10.1155/2012/578925
Guven, H, Dereli, RK, Ozgun, H, Ersahin, ME & Ozturk, I 2019, Towards sustainable and energy efficient municipal wastewater treatment by up-concentration of organics. Progress in Energy and Combustion Science, 70: 145-168, DOI: 10.1016/j.pecs.2018.10.002.
Hall, TA 1999, BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. In Nucleic Acids Symposium Series, 41: 95-98, [London]: Information Retrieval Ltd.: 1979-2000.
Holatko, J, Bielska, L, Hammerschmiedt, T, Kucerik, J, Mustafa, A, Radziemska, M & Brtnicky, M 2022, Cattle manure fermented with biochar and humic substances improve the crop biomass, microbiological properties and nutrient status of soil. Agronomy, 12: 368, DOI: 10.3390/agronomy12020368,
Khan, AAH 2019, Plant-bacterial association and their role as growth promoters and biocontrol agents. In: RZ, Sayyed, (Ed.), Plant growth promoting rhizobacteria for sustainable stress management: Rhizobacteria in biotic stress management, Vol. 13, Springer, Singapore, 389-419,
Khanna, K, Kohli, SK, Kaur, R, Handa, N, Bakshi, P, Sharma, P & Bhardwaj, R 2022, Reconnoitering the efficacy of plant growth promoting rhizobacteria in expediting phytoremediation potential of heavy metals. Journal of Plant Growth Regulation, 1-29, DOI: 10.1007/s00344-022-10879-9.
Kocak, FO 2023, Simultaneous evaluation of composting experiments and metagenome analyses to illuminate the effect of Streptomyces spp. on organic matter degradation. World Journal of Microbiology and Biotechnology, 39: 70, DOI: 10.21203/  
Kunova, A, Cortesi, P, Saracchi, M, Migdal, G & Pasquali, M 2021, Draft genome sequences of two Streptomyces albidoflavus strains DEF1AK and DEF147AK with plant growth-promoting and biocontrol potential. Annals of Microbiology, 71: 1-8, DOI: 10.1186/s13213-020-01616-2.
Mohamed, AAE, Altaii, HZ & Ibrahim, T 2023, First record of the bacterium Pseudomonas putida on pepper in Iraq. Caspian Journal of Environmental Sciences, 21: 473-480.
Orozco Mosqueda, MDC, Santoyo, G & Glick, BR 2023, Recent Advances in the Bacterial Phytohormone Modulation of Plant Growth. Plants, 12: 606, DOI: 10.3390/plants12030606.
Ortiz Castro, R, Campos García, J & López Bucio, J 2020, Pseudomonas putida and Pseudomonas fluorescens influence Arabidopsis root system architecture through an auxin response mediated by bioactive cyclodipeptides. Journal of Plant Growth Regulation, 39: 254-265. DOI: 10.1007/s00344-019-09979-w
Othman, YA, Al Assaf, A, Tadros, MJ & Albalawneh, A 2021, Heavy metals and microbes accumulation in soil and food crops irrigated with wastewater and the potential human health risk: A metadata analysis. Water, 13: 3405, DOI: 10.3390/w13233405.
Pang, L, Ge, L, Yang, P, He, H & Zhang, H 2018, Degradation of organophosphate esters in sewage sludge: effects of aerobic/anaerobic treatments and bacterial community compositions. Bioresource Technology, 255: 16-21, DOI: 10.1016/j.biortech.2018.01.104
Parhamfar, M., Abtahi, H., Godini, K., Saeedi, R, Sartaj, M, Villaseñor, J & Koolivand, A 2020, Biodegradation of heavy oily sludge by a two-step inoculation composting process using synergistic effect of indigenous isolated bacteria. Process Biochemistry, 91: 223-230, DOI: 10.1016/j.procbio.2019.12.014.
Ravintheran, SK, Sivaprakasam, S, Loke, S, Lee, SY, Manickam, R, Yahya, A & Rajandas, H 2019, Complete genome sequence of Sphingomonas paucimobilis AIMST S2, a xenobiotic-degrading bacterium. Scientific data, 6: 280, DOI: 10.1038/s41597-019-0289-x.
Safwat, SM & Matta, ME 2021, Environmental applications of effective microorganisms: A review of current knowledge and recommendations for future directions. Journal of Engineering and Applied Science, 68: 1-12, DOI: 10.1186/s44147-021-00049-1.
Saravanan, A, Thamarai, P, Kumar, PS & Rangasamy, G 2022, Recent advances in polymer composite, extraction, and their application for wastewater treatment: A review. Chemosphere, 136368. DOI: 10.1016/j.chemosphere.2022.136368
Shalaby, EA 2011, Prospects of effective microorganisms technology in wastes treatment in Egypt. Asian Pacific Journal of Tropical Biomedicine, 1: 243-248. DOI: 10.1016/S2221-1691(11)60035-X
Singh, A 2021, A review of wastewater irrigation: Environmental implications. Resources, Conservation and Recycling, 168: 105454. DOI:  10.1016/j.resconrec.2021.105454
Singh, J, Kumar, S & Sharma, S 2022, Biopolymer in wastewater treatment. In: Biopolymers: Recent updates, challenges and opportunities, pp. 323-351, Cham: Springer International Publishing. DOI: 10.1007/978-3-030-98392-5_15
Vaz Jauri, P, Beracochea, M, Fernández, B & Battistoni, F 2019, Whole-genome sequencing of Streptomyces sp. strain UYFA156, a cultivar-specific plant growth-promoting endophyte of Festuca arundinacea. Microbiology Resource Announcements, 8: pp.e00722-19, DOI: 10.1128/MRA.00722-19
Wang, S, Na, X, Yang, L, Liang, C, He, L, Jin, J, Liu, Z, Qin, J, Li, J, Wang, X & Bi, Y 2021, Bacillus megaterium strain WW1211 promotes plant growth and lateral root initiation via regulation of auxin biosynthesis and redistribution. Plant and Soil, 466: 491-504. DOI: 10.1007/s11104-021-05055-z
Zabolotskikh, VV, Vasilyev, AV & Tankikh, SN 2019, Development of bioactive composition for effective bioremediation of oil-contaminated soil. In: Proceedings of the 7th International Environmental Congress (9th International Scientific-Technical Conference)" Ecology and life protection of industrial-transport complexes" ELPIT: 173-190.
Zhang, C, Yang, X, Tan, X, Wan, C & Liu, X 2022, Sewage sludge treatment technology under the requirement of carbon neutrality: Recent progress and perspectives. Bioresource Technology, 362: 127853. DOI: 10.1016/j.biortech.2022.127853
Zhang, H, Dong, S, Lou, T & Wang, S 2019, Complete genome sequence unveiled cellulose degradation enzymes and secondary metabolic potentials in Streptomyces sp. CC0208. Journal of Basic Microbiology, 59: 267-276 DOI: 10.1002/jobm.201800563
Zhang, J, Hussain, S, Zhao, F, Zhu, L, Cao, X, Yu, S & Jin, Q 2018, Effects of Azospirillum brasilense and Pseudomonas fluorescens on nitrogen transformation and enzyme activity in the rice rhizosphere. Journal of Soils and Sediments, 18: 1453-1465, DOI: 10.1007/s11368-017-1861-7.
Zhao, Y, Li, W, Chen, L, Meng, L & Zheng, Z 2020, Effect of enriched thermos-tolerant nitrifying bacteria inoculation on reducing nitrogen loss during sewage sludge composting. Bioresource Technology, 311: 123461. DOI: 10.1016/j.biortech.2020.123461.
Zheng, W et al. 2022, Roles of organic matter transformation in the bioavailability of Cu and Zn during sepiolite-amended pig manure composting. Journal of Environmental Management, 314: 115046, DOI: 10.1016/j.jenvman.2022.115046.
Zhou, X, Li, J, Zhang, J, Deng, F, Chen, Y, Zhou, P & Li, D 2022, Bioaugmentation mechanism on humic acid formation during composting of food waste. Science of the Total Environment, 830: 154783, DOI: 10.1016/j.scitotenv.2022.154783.
Zinchenko, MK, Zinchenko, SI, Mazirov, MA, Ragimov, AO & Shitikova, AV 2021, Biological indicators in the environmental monitoring of gray forest soil of agrosystems. Caspian Journal of Environmental Sciences, 19: 891-896.