Antibacterial effects of bioactive metabolites extracted from Nocardia pseudobrasiliensis

Document Type : Research Paper


1 Department of Microbiology, North Tehran Branch, Islamic Azad University, Tehran, Iran

2 Department of Pathobiology, School of Public Health, Tehran University of Medical Science, Tehran, Iran

3 Department of Microbiology, Faculty of Basic Sciences, North Tehran Branch, Islamic Azad University, Tehran, Iran

4 Department of Medical Entomology and Vector Control, Tehran University of Medical Sciences, School of Public Health, Tehran, Iran

5 Department of Epidemiology and Biostatistics, Tehran University of Medical Sciences, Tehran, Iran



Our study was designed to examine the antimicrobial activity of an Actinobacteria strain previously isolated from soil. In the soil surrounding lemon beebrush (Aloysia citriodora L.), a Nocardia isolate was identified. This strain was evaluated using phenotypic, biochemical, and molecular methods and was distinguished as Nocardia pseudobrasiliensis. The structure of the metabolite was determined using mass spectrometry (MS). The antibacterial activity of this strain was detected during primary screening and was observed to exhibit strong antibacterial effects against important nosocomial infections. These bacterial strains were tested to determine their minimum inhibitory concentration (MIC). The metabolite was first distinguished in N. pseudobrasiliensis. Phylogenetic and biochemical analyses showed that the isolated strain is similar to N. pseudobrasiliensis. The possible molecular structure of the metabolite was distinguished as C18H36O2. Based on these results, the metabolite appears to have strong antimicrobial activity both against Gram-positive and -negative bacteria. Despite the fact that N. pseudobrasiliensis was found in the soil surrounding the lemon beebrush roots, further investigations are necessary in order to determine whether it may be an endophyte or a soil microorganism. In the present study, the C18H36O2 compound in the N. pseudobrasiliensis is reported for the first time.


Ali, H, EL Beltagi, H, Nasr, N 2008, Assessment of volatile components, free radical-scavenging capacity and anti-microbial activity of lemon verbena leaves. Research Journal of Phytochemistry, 2: 84-92.
Ali, N, Muhammad, A, Ali, I, Owais, M, Khan, SN, Afridi, IQ 2020, Evaluation of antibiotics pattern of extended spectrum beta- lactamase producing multi- drug resistant Pseudomonas aeruginosa. Advancements in Life Sciences, 7: 146-150.
Aoki, H, Sakai, HI, Kohsaka, M, Konomi, T, Hosoda, J, Kubochi, Y, IguchiI, E & Imanaka, H 1976, Nocardicin A, a new monocyclic β-lactam antibiotic I. Discovery, isolation and characterization. The Journal of antibiotics, 29: 492-500.
Beiranvand, M, Amin, M, Hashemi-Shahraki, A, Romani, B, Yaghoubi, S & Sadeghi, P 2017, Antimicrobial activity of endophytic bacterial populations isolated from medical plants of Iran. Iranian journal of microbiology, 9: 11-18.
Bell, M, Mcnell, MM & Brown, JM 2014: Nocardia species (Nocardiosis). Antimicrobe. Available online: http://www. antimicrobe. org/b117. asp (accessed on 18 August 2020).
Borquaye, LS, Darko, G, Oklu, N, Anson Yevy, C & Ababio, A 2016, Antimicrobial and antioxidant activities of ethyl acetate and methanol extracts of Littorina littorea and Galatea paradoxa. Cogent Chemistry, 2: 1161865.
BSSN, HB, Muvva, V, Munaganti, RK, Naragani, K, Konda, S & Doragondla, KR 2017, Production of antimicrobial metabolites by Streptomyces lavendulocolor VHB-9 isolated from granite mines. Brazilian Archives of Biology and Technology, 60.
Celmer, W, Chmurny, G, Moppett, CE, Ware, R, Watts, PT & Whipple, E 1980, Structure of natural antibiotic CP-47,444. Journal of the American Chemical Society, 102: 4203-4209.
Chun, J & Goodflow, M 1995, A phylogenetic analysis of the genus Nocardia with 16S rRNA gene sequences. International Journal of Systematic and Evolutionary Microbiology, 45: 240-245.
Da Silva, LL, Nascimento, MS, Cavaleria, AJ, Silva, DH, Castro-Gamboa, I, Furlan, M & Bolzanivda, S 2008, Antibacterial activity of Labdane diterpenoids from Stemodia foliosa. Journal of Natural Products, 71: 1291-3.
Danser, SJ 2004, How antibiotics can make us sick: the less obvious adverse effects of antimicrobial chemotherapy. Lancet Infectious Diseases, 4: 611-9.
Daveis, J 1999, Millennium bugs. Trends in Genetics, 15: M2-M5.
DE Oliveira, AIT, Cabral, JB, Mahmoud, TS, DO Nascimento, GNL, DA Silva, JFM, Pimenta, RS & DE Morias, PB 2017. In vitro antimicrobial activity and fatty acid composition through gas chromatography-mass spectrometry (GC-MS) of ethanol extracts of Mauritia flexuosa (Buriti) fruits. Journal of Medicinal Plants Research, 11: 635-641.
Gandra, S, Barter, D & Laxminarayan, R 2014, Economic burden of antibiotic resistance: how much do we really know? Clinical Microbiology and Infection, 20: 973-980.
Goswami, C, Fox, S, Holdieh, MT, Connor, M, Leanord, A & Evans, TJ 2020, Origin, maintenance and spread of antibiotic resistance genes within plasmids and chromosomes of bloodstream isolates of Escherichia coli. Microbial genomics, 6.
Habibnia, S, Nanas, MR, Heidarieh, P, Bsafghi, MF, Pourmand, MR & Eshraghi, SS 2015, Phenotypic characterization of Nocardia spp. isolated from Iran soil microflora. International Journal of Environmental Health Engineering, 4: 20-32.
Hashemi, M, Ehsani, A, Hassani, A, Afshari, A, Aminzare, M, Sahranavard, T & Azimzadeh, Z 2017, Phytochemical, antibacterial, antifungal and antioxidant properties of Agastache foeniculum essential oil. Journal of Chemical Health Risks, 7(2), DOI: 10.22034/JCHR.2017.544170.
Hassan, A, El Barawy, A & EL Mokhtar, MN 2011, Evaluation of biological compounds of Streptomyces species for control of some fungal diseases. The Journal of American Science, 7: 752-60.
Hayakawa, M & Nonoruwa, H 1987, Humic acid-vitamin agar, a new medium for the selective isolation of soil actinomycetes. Journal of Fermentation Technology, 65: 501-509.
Hutchings, MI, Truman, AW & Wilkinson, B 2019, Antibiotics: past, present and future. Current Opinion in Microbiology, 51: 72-80.
Javed, M, Bibi, R, Nazir, K & Hussain S 2022, Phytochemistry of Ziziphus mauritiana; its antioxidant and antimicrobial potential. Advancements in Life Sciences, 27, 9:157-62.
Jensen, PR, Dwight, R & Fenical, W 1991, Distribution of actinomycetes in near-shore tropical marine sediments. Applied and Environmental Microbiology, 57: 1102-1108.
Kavitha, A, Prabhakar, P, Narasimhulu, M, Vijayalakshmi, M, Venkateswarlu, Y, Venkateswara Rao, K & Subba Raju, B 2010, Isolation, characterization and biological evaluation of bioactive metabolites from Nocardia levis MK-VL_113. Microbiological Research, 165: 199-210.
Kavitha, A, Prabhakar, P, Vijayalakshmi, M, Venkateswarlu, Y 2009, Production of bioactive metabolites by Nocardia levis MK-VL_113. Letters in Applied Microbiology, 49: 484-90.
Kiska, DL, Hicks, K & Pettit, DJ 2002, Identification of medically relevant Nocardia species with an abbreviated battery of tests. Journal of Clinical Microbiology, 40: 1346-1351.
Lerminiaux, NA & Cameron, ADS 2019, Horizontal transfer of antibiotic resistance genes in clinical environments. Canadian Journal of Microbiology, 65: 34-44.
Livermore, DM 2012, Current epidemiology and growing resistance of gram-negative pathogens. The Korean Journal of Internal Medicine, 27: 128-42.
Miller, CS, Handley, KM, Wrighton, KC, Frischkorn, KR, Thomas, BC & Banfield, JF 2013, Short-read assembly of full-length 16S amplicons reveals bacterial diversity in subsurface sediments. PLoS One, 8: e56018.
Mukai, A, Fukai, T, Hoshino, Y, Yazawa, K, Harada, KI & Mikami, Y 2009, Nocardithiocin, a novel thiopeptide antibiotic, produced by pathogenic Nocardia pseudobrasiliensis IFM 0757. The Journal of Antibiotics, 62: 613-619.
Paul, M, Shani, V, Muchtar, E, Kariv, G, Robenshtok, E & Leibovici, L 2010, Systematic review and meta-analysis of the efficacy of appropriate empiric antibiotic therapy for sepsis. Antimicrobial Agents and Chemotherapy, 54: 4851-63.
Ramazani, A, Moradi, S, Sorouri, R, Javani, S & Garshasbi, M 2013, Screening for antibacterial activity of Streptomyces species isolated from Zanjan Province, Iran. International Journal of Pharmacy and Biological Sciences, 3: 342-349.
Rahman, A, Rauf, A, Ali, B, Ullah, M, Ali, M et al. 2022, Phytochemical analysis and antibacterial activity of Berberis vulgaris extract. Adv. Life Sciences, 9: 289-294.
Rana, S & Salam, M 2014, Antimicrobial potential of actinomycetes isolated from soil samples of Punjab. Indian Journal of Microbiology & Experimentation, 1: 00010.
Rezamahalleh, HM, Khodakaramian, G & Hassanzadeh, N 2019, Diversity of endophytic and epiphytic bacteria from sugarcane in Khuzestan, Iran. Brazilian Archives of Biology and Technology, 62,
Ruimy, R, Riegel, P, CarlottiI, A, Boiron, P, Bernardin, G, Monteil, H, Wallace JRJ & Chreistin, R 1996, Nocardia pseudobrasiliensis sp. nov., a new species of Nocardia which groups bacterial strains previously identified as Nocardia brasiliensis and associated with invasive diseases. International Journal of Systematic and Evolutionary Microbiology, 46: 259-264.
Singh, M, Andhu, RS & Randhaha, HS 1987, Comparison of paraffin baiting and conventional culture techniques for isolation of Nocardia asteroides from sputum. Journal of Clinical Microbiology, 25: 176-177.
Singh, R & Dubey, AK 2018, Diversity and applications of endophytic actinobacteria of plants in special and other ecological niches. Frontiers in Microbiology, 9: 1767-1767.
Sullivan, DC & Chapman, SW 2010, Bacteria that masquerade as fungi: actinomycosis/nocardia. Proceedings of the American Thoracic Society, 7: 216-21.
Tan, RX, Zou, WX 2001, Endophytes: a rich source of functional metabolites. Natural Product Reports, 18: 448-59.
Wallace, RJ, JR., Brown, BA, Blachlouk, Z, Ulrich, R, Jost, K, Brown, JM, Mcneil, MM, Onli, G, Steinrube, VA & Gibson, J 1995, New Nocardia taxon among isolates of Nocardia brasiliensis associated with invasive disease. Journal of Clinical Microbiology, 33: 1528-1533.
Yu, H, Zhang, L, Li, L, Zheng, C, Guo, L, Li, W, Sun, P & Qin, L 2010, Recent developments and future prospects of antimicrobial metabolites produced by endophytes. Microbiological Research, 165: 437-49.