Efficiency of aqueous and alcoholic extract of Costus speciosus roots on Iraqi pneumonic isolated bacteria

Document Type : Research Paper

Author

Department of Biology, College of Science for Women, University of Baghdad, Iraq

10.22124/cjes.2023.6706

Abstract

The decorative, pharmaceutical plant, Costus speciosus can be useful for health as therapeutic agent. This study tends to estimate the antibacterial activity of this plant rhizomes (aqueous and methanol extract) on   Pseudomonas aeruginosa growth using the well diffusion method. In this study, the C. speciosus sundried rhizomes were powdered and extracted using Soxhlet apparatus, diluted for specific concentrations of the collected dried extract, then qualitative detection in raw extract of the plant rhizomes was performed for detection of the active compound’s presence. The P. aeruginosa which is pathogenic bacteria, was placed in a culture of brain heart infusion broth and adjusted to McFarland tube. The resulted extract was two kinds (three samples for each concentration), initial concentration was adjusted to 200 mg mL-1.  Measurement of zones of inhibition were performed after incubation, followed by statistical collection and analysis of data.  Five compounds in the C. speciosus rhizomes methanol extract were present using the qualitative detection of the active compounds including alkaloids, saponites, turbines, tannins, and flavonoids. The extracts showed influential inhibition of growth of bacterium used in this study. The methanol and aqueous extracts with the concentration of 200 µg mL-1 showed the maximum antibacterial effect with the zone inhibition diameters of 16 and 17 mm respectively. The methanol extract was moderately effective with the concentration of 100 µg mL-1 and the inhibition zone diameter of about 10 mm.

Keywords

References

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Available Online from 10 June 2023

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