Evaluation of Pediococcus acidilactici from Dadiah Bukitinggi (dairy food) as an insulin promotor by bioinformatics

Document Type : Research Paper


1 Lincoln University College, Wisma Lincoln, No. 12-18, SS 6/12, Off Jalan Perban daran, 47301 Petaling Jaya, Selangor DE; Medical Faculty, Baiturrahmah Uni versity Padang, West Sumatera, Indonesia

2 Deputy Vice Chancellor of Research, Lincoln University College, Malaysia

3 Faculty of Animal Science, Andalas University, Padang, 21566, Indonesia



Dadiah is one of traditional food in West Sumatera. It is made from naturally fermented buffalo milk in bamboo tubes by lactic acid bacteria found naturally in the buffalo milk and bamboo tubes themselves. Lactic  acid bacteria strains are the main probiotics in the food and pharmaceutical markets. Probiotics are live microorganisms or components of a microbial cell that give good effect to other organisms. Probiotics have an important role in the health of the gastrointestinal tract in general. Pediococcus acidilactici is one of the lactic acid bacteria that acts as a probiotic isolated from Dadiah. Insulin is a hormone produced by pancreatic beta cells, which plays an important role in regulating blood glucose levels, so that they remain in a normal state, and its promoter regulates the rate of transcription in response to physiological regulators. In human pancreatic cells, the insulin promoter interacts with loci associated with diabetes risk and controls genes involved in insulin metabolism. Insulin promoters are tissue-specific and typically only activate in islet beta cells in adult tissues to stimulate insulin production. This study aims to determine the secondary metabolites of P. acidilactici to insulin promotor expression by looking at the canonical and isomeric SMILE (simplified molecular-input line-entry system) structures in the PubChem database. After conducting bioinformatics analysis to determine the secondary metabolites of P. acidilactici, its compound has potential as a diabetes treatment, because the QSAR results show potential as an insulin promoter (0.75).


Akash, MSH, Rehman, K & Liaqat, A 2018, Tumor necrosis factor‐alpha: role in development of insulin resistance and pathogenesis of type 2 diabetes mellitus. Journal     of Cellular Biochemistry, 119: 105-110.
Albensi, BC 2019, What is nuclear factor kappa B (NF-κB) doing in and to the mitochondrion. Frontiers in Cell and Developmental Biology, 7: 154.
Ashouri, G, Mahboobi Soofiani, N, Hoseinifar, SH, Jalali, SAH, Morshedi, V, Valinassab, T, Bagheri, D, Van Doan, H, Torfi Mozanzadeh, M & Carnevali, O 2020, Influence of dietary sodium alginate and Pediococcus acidilactici on liver antioxidant status, intestinal lysozyme gene expression, histomorphology, microbiota, and digestive enzymes activity, in Asian sea bass (Lates calcarifer) juveniles. World Journal of Gastrointestinal Pathophysiology, https://www.wjgnet.com› CitedArticlesInF6.
B 2003, Regulation of adiponectin by adipose tissue-derived cytokines: In vivo and in vitro investigations in humans. American Journal of Physiology - Endocrinology and Metabolism, 285: 527-533.
Bruun, JM, Lihn, AS, Verdich, C, Pedersen, SB, Toubro, S, Astrup, A & Richelsen, A 2022, Aquaculture, 518 (October), 734638.
Calle, MC & Fernandez, ML 2012, Inflammation and type 2 diabetes. Diabetes and    Metabolism, 38: 183-191. https://doi.org/10.1016/j.diabet.2011.11.006
Filimonov, DA, Lagunin, AA, Gloriozova, TA, Rudik, AV, Druzhilovskii, DS, Pogodin, PV & Poroikov, VV 2014, Prediction of the biological activity spectra of organic   compounds using the PASS online web resource. Chemistry of Heterocyclic Compounds, 50: 444-457.
Firouzi, S, Majid, HA, Ismail, A, Kamaruddin, NA & Barakatun Nisak, MY 2017, Effect of multi-strain probiotics (multi-strain microbial cell preparation) on glycemic control and other diabetes-related outcomes in people with type 2 diabetes: a randomized controlled trial. In European Journal of Nutrition, 56: 1535-1550. https://doi.org/10.1007/s00394-016-1199-8
Helmizar, H & Surono, IS 2019, Characteristics of amino acid, micronutrient and probiotic isolated from dadiah and their benefits for pregnant mothers and outcomes in West Sumatra, Indonesia. Global Journal of Health Science, 12: 116.
Herder, C, Brunner, EJ, Rathmann, W, Strassburger, K, Tabák, AG, Schloot, NC &      Witte, DR 2009, Elevated levels of the anti-inflammatory interleukin-1 receptor antagonist precede the onset of type 2 diabetes: The whitehall II study. Diabetes Care,      32: 421-423, https://doi.org/10.2337/dc08-1161
Hussain SP & Harris CC 2006, p53 biological network: at the crossroads of the cellular-stress response pathway and molecular carcinogenesis. Journal of Nippon Medical School. 73: 54- 64. DOI: 10.1272/jnms.73.54. PMID: 16641528.
Khalili, L, Alipour, B, Jafar Abadi, MA, Faraji, I, Hassanalilou, T, Abbasi, MM, Vaghef Mehrabany, E & Sani, MA 2019, The effects of Lactobacillus casei on glycemic response, serum sirtuin1 and fetuin-A levels in patients with type 2 diabetes mellitus: A randomized controlled trial. Iranian Biomedical Journal, 23: 68-77. https://doi.org/10.29252/IBJ.23.1.68
Kobyliak, N, Conte, C, Cammarota, G, Haley, AP, Styriak, I, Gaspar, L, Fusek, J, Rodrigo, L & Kruzliak, P 2016, Probiotics in prevention and treatment of obesity: A    critical view. Nutrition and Metabolism, 13, https://doi.org/10.1186/s12986-016- 0067-0
Ljungh, Å & Wadström, T 2001, Lactic acid bacteria as probiotics further reading. Current Issues Intestinal Microbiology, 7: 73-90.
Mobini, R, Tremaroli, V, Ståhlman, M, Karlsson, F, Levin, M, Ljungberg, M, Sohlin, M,    Bertéus Forslund, H, Perkins, R, Bäckhed, F & Jansson, PA 2017, Metabolic effects of Lactobacillus reuteri DSM 17938 in people with type 2 diabetes: A randomized controlled trial. Diabetes, Obesity and Metabolism, 19: 579-589, https://doi.org/10.1111/dom.12861
Perner, F, Perner, C, Ernst, T & Heidel, FH 2019, Roles of JAK2 in aging, inflammation, hematopoiesis and malignant transformation. Cells, 8: 854,  https://doi.org/10.3390/ cells808 0854
Pini, A, Obara, I, Battell, E, Chazot, PL & Rosa, AC 2016, Histamine in diabetes: is it  time to reconsider? Pharmacological Research, 111: 316-324.
Purwati RN, Aritonang, SN, Melia, S, Juliyarsi, I & Purwanto, H 2016, Probiotic benefits of dadiah's lactic acid bacteria support public health. RMS, Putra (ed.), Information and Communication Technology Development Institute (LPTIK) Unand, Indonesia.
Ramakrishnan, U 2010, A review of the benefits of nutrient supplements during pregnancy:  From iron-folic-acid to long-chain polyunsaturated fatty acids to probiotics. Annales Nestle, 68: 29-40. https://doi.org/10. 1159/000298781
Razmpoosh, E, Javadi, A, Ejtahed, HS, Mirmiran, P, Javadi, M & Yousefinejad, A 2019, The effect of probiotic supplementation on glycemic control and lipid profile in patients with type 2 diabetes: A randomized placebo controlled trial. Diabetes and Metabolic Syndrome: Clinical Research and Reviews, 13: 175-182. https://doi.org/10.1016/j.dsx.2018.08.008
Rhadhiyah, A 2008, Existence and fermentative potential of dadiah isolates in several regencies in West Sumatra. Andalas University, Indonesia.
Rodrigues, MAM, Silva, DAO, Taketomi, EA & Hernandez Blazquez, FJ 2007, IgA production, coliforms analysis and intestinal mucosa morphology of piglets that received probiotics with viable or inactivated cells. Pesquisa Veterinaria Brasileira,      27: 241-245, https://doi.org/10.1590/S0100-736X2007000600004
Scharl, M 2015, Pathophysiological role of TNF in inflammatory bowel disease: TNF and its effect on innate immune defense. In Anti-tumor necrosis factor therapy in inflammatory bowel disease. Karger Publishers, 34: 49-55
Surono, IS 2015, Indonesian Dadih. In: AK Puniya (Ed.), fermented milk and dairy    products, pp. 377-339. https://doi.org/10.1201/b18987-20
Surono, IS 2016, Ethnic fermented foods and alcoholic beverages of Asia. In: Ethnic fermented foods and alcoholic beverages of Asia, pp. 341-382,  Springer India. https://doi.org/10.1007/978-81-322-2800-4
Tan, K, Deng, D, Ma, X, Cui, Y & Tian, Z 2020, Pediococcus acidilactici P25 protected  Caenorhabditis elegans against enterotoxigenic Escherichia coli K88 infection and transcriptomic analysis of its potential mechanisms. BioMed Research International, https://doi.org/10.1155/2020/7340312
Tao, YW, Gu, YL, Mao, XQ, Zhang, L & Pei, YF 2020, Effects of probiotics on type II diabetes mellitus: A meta-analysis. Journal of Translational Medicine, 18: 1- 11, https://doi.org/10.1186/s12967-020-02213-2
Thirone, AC, JeBailey, L, Bilan, PJ & Klip, A 2006, Opposite effect of JAK2 on insulin-dependent activation of mitogen-activated protein kinases and AKT in muscle    cells: possible target to ameliorate insulin resistance. Diabetes, 55: 942-951.
Wang, J, Qin, J, Li, Y, Cai, Z, Li, S, Zhu, J, Zhang, F, Liang, S, Zhang, W, Guan, Y, Shen, D, Peng, Y, Zhang, D, Jie, Z, Wu, W, Qin, Y, Xue, W, Li, J, Han, L, et al. 2012, A metagenome-wide association study of gut microbiota in type 2  diabetes. Nature, 490: 55-60, https://doi.org/10.1038/nature11450
Wang, S, Yao, B, Gao, H, Zang, J, Tao, S, Zhang, S, Huang, S, He, B, & Wang, J 2019, Combined supplementation of Lactobacillus fermentum and Pediococcus acidilactici promoted growth performance, alleviated inflammation, and modulated intestinal microbiota in weaned pigs. BMC Veterinary Research, 15: 1–11.