Direct transesterification to produce bio-diesel from Oedogonium algae and duckweed plant

Document Type : Research Paper

Authors

1 College of Dentistry, University of Thi-Qar, Thi-Qar, 64001, Iraq

2 Department of Chemistry, College of Science, University of Thi-Qar, Thi-Qar, 64001, Iraq

3 College of Engineering, University of Thi-Qar, Thi-Qar, 64001, Iraq

10.22124/cjes.2023.6230

Abstract

Biodiesel from renewable sources is one of the possible alternatives support the energy security. This study aimed to produce biodiesel from Oedogonium algae and duckweed plant by direct transesterification. An amount of 5 g of dry biomass was taken for the considered duckweed plant and the algae was immersed in 50 mL of methanol: hydrochloric acid: chloroform (10:1:1 v/v/v)   solvent to extract fatty acid methyl esters (FAME).  The extracted materials were taken for the characterisation using FT-IR and GC-Mass spectrometry. The prepared samples were specifically diagnosed for the identification of compounds particularly lipids. The results showed presence of saturated and unsaturated fatty acids in the formed ester (biodiesel). Saturated and unsaturated fatty acids identified in the biodiesel derived from duckweed plant were 24.19% and 20.34% respectively and from Oedogonium algae were 19.92% and 17.2% respectively. These results demonstrated a high potential to produce biodiesel from these types of biomass which could provide another route for energy supply.

Keywords


Alaswad A, Dassisti, M, Prescott, T & Olabi AG 2015, Technologies and developments of third generation biofuel production. Renewable and Sustainable Energy Reviews, 51: 144660
Al-Graiti, W & Merdas, SM 2023, evaluation of some eco-friendly food residues for removal of methylene blue dye from aqueous solutions. Journal of Medicinal and Chemical Sciences, 6: 1044-1054
Asiye Gül Bayrakci, AG & Kocar, G 2014, Second-generation bioethanol production from water hyacinth and duckweed in Izmir: A case study. Renewable and Sustainable Energy Reviews, 30: 306-316.
Brennan, L & Owende, P 2010, Biofuels from microalgae: A review of technologies for production, processing, and extractions of biofuels and co-products. Renewable and Sustainable Energy Reviews, 14: 557-577.
Chew, KW, Yap, JY, Show, PL, Suan, NH, Juan, JC, Ling, TC & Chang, JS 2017, Microalgae bio-refinery: high value products perspectives. Bioresource Technology, 229: 53-62.
Chisti, Y 2007, Biodiesel from microalgae. Biotechnology Advances, 25: 294-306.
Dubé, MA, Tremblay, AY & Liu, J 2007, Biodiesel production using a membrane reactor. Bioresource Technology, 98: 639-647.
Ethaib, S, Omar, R, Mustapa Kamal, SM & Awang Biak, DR 2020a, Comparison of sodium hydroxide and sodium bicarbonate pretreatment methods for characteristic and enzymatic hydrolysis of sago palm bark. Energy Sources, Part A: Recovery, Utilization, and Environmental Effects, 1-11. https://doi.org/10.1080/ 15567036.2020.1753857.
Ethaib, S, Omar, R, Siti Mazlina, MK & Dayang Radiah, AB2020b, Evaluation of the interactive effect pre-treatment parameters via three types of microwave-assisted pre-treatment and enzymatic hydrolysis on sugar yield. Processes, 8: 787.
Ethaib, S, Omar, R, Mazlina, MS, Radiah, AD & Zubaidi, SL 2020c, Toward sustainable processes of pretreatment technologies of lignocellulosic biomass for enzymatic production of biofuels and chemicals: a review. BioResources, 15: 10063.
Gajraj RS, Singh GP & Kumar A 2018, Third-generation biofuel: algal biofuels as a sustainable energy source. In: Kumar, et al. editors. Biofuels: greenhouse gas mitigation and global warming, A. Springer India Pvt. Ltd., p. 30725.
Ge, X, Zhang, N, Phillips, GC & Xu, J 2012, Growing Lemna minor in agricultural wastewater and converting the duckweed biomass to ethanol. Journal of Bioresource Technology 124:  485-488.
Guimarães, J, Cursino, A, Saul, C, Sierakowski, M, Pereira Ramos, L & Satyanarayana, KG  2016, Evaluation of Castor Oil Cake Starch and Recovered Glycerol and Development of “Green” Composites Based on Those with Plant Fibers. Materials, 9: 76.
Kamil, JM & Taha, TN 2022 Effects of substituting different percentages of Azolla and duckweed as a partial substitute for soybean meal in feeding common carp fish (Cyprinus carpio L.). Caspian Journal of Environmental Sciences, 20: 593-601.
Khaliullina, LY 2021, Comparative analysis of the structure of planktonic algae of the Volga and Kama rivers before their confluence in the Kuibyshev reservoir (the Republic of Tatarstan, RF). Caspian Journal of Environmental Sciences, 19: 861-870.
Khilji, S, Munir, N, Aziz, I, Anwar, B, Hasnain, M, Jakhar, A, Sajid, Z, Abideen, Z, Hussain, MI, El-Habeeb, A & Yang, HH  2022, Application of Algal Nanotechnology for Leather Wastewater Treatment and Heavy Metal Removal Efficiency." Sustainability, 14: 13940.
Lim, L, Priyantha, N, Chan, C, Matassan, D, Hei Ing, C, & Kooh, MRR  2014, Adsorption Behaviour of Methyl Violet 2B Using Duckweed: Equilibrium and Kinetics Studies. Arabian Journal for Science and Engineering, 39.
Mohedano, RA, Costa, RH, Tavares, FA & Filho, PB  2012, High nutrient removal rate from swine wastes and protein biomass production by full-scale duckweed ponds, Bioresource Technology, 112: 98-104.
Naylor, RL, Liska, AJ, Burke, MB, Falcon, WP, Gaskell, JC, Rozelle, SD & Cassman, KG 2007, The ripple effect: biofuels, food security, and the environment. Environment 49: 30-43.
 Neto, JM, Komesu, A, Martins, LHDS, Gonçalves, VO, De Oliveira, JAR & Rai, M 2019, Third-generation biofuels: An overview. Sustainable Biofuel and Biomass, Apple Academic Press, 20 p.
Newete, SW & Byrne, MJ 2016. The capacity of aquatic macrophytes for phytoremediation and their disposal with specific reference to water hyacinth. Environmental Science and Pollution Research, 23: 10630-10643.
Park, JY, Park, MS, Lee, YC & Yang, JW 2015, Advances in direct transesterification of algal oils from wet biomass. Bioresource Technology, 184: 267-275.
Rezania, S, Taib, SM, Md Din, MF, Dahalan, FA & Kamyab, H 2016, Comprehensive review on phyto-technology: Heavy metals removal by diverse aquatic plants species from wastewater. Journal of Hazardous Materials, 318: 587-599.
Rios, SD, Castañeda, J, Torras, C, Farriol, X & Salvadó, J 2013, Lipid extraction methods from microalgal biomass harvested by two different paths: Screening studies toward biodiesel production. Bioresource Technology, 133: 378-388.
Saifuddin, NM, Hussein, R & Palanisamy, K 2014, Rapid purification of glycerol by-product from biodiesel production through combined process of microwave assisted acidification and adsorption via chitosan immobilized with yeast. Research Journal of Applied Sciences, Engineering and Technology, 7: 593-602.
Sayer, AH, Al-Hussaini, H & Campbell, AN 2017, An analytical estimation of salt concentration in the upper and lower convective zones of a salinity gradient solar pond with either a pond with vertical walls or trapezoidal cross section. Solar Energy, 158: 207-2017.
Sayer, AH & Mahood, HB 2020, The effect of a liquid cover on the thermal performance of a salinity gradient solar pond: An experimental study. Energy Engineering, 119: 367-379, DOI: 10.32604/EE. 2022.017715
Sati, H, Madhusree, M, Sandhya, M & Prashant B 2019, Microalgal lipid extraction strategies for biodiesel production: A review." Algal Research, 38: 101413.
Wang, Y, Wang, X, Yang, R, Niu, L & Wang, W 2020, Comparison of protein extraction methods for 2DE-based proteomic analysis of duckweed Spirodela polyrhiza, a small aquatic model plant. Aquatic Botany, 163: 103216.
Yousuf, A, Khan, MR, Islam, MA, Wahid, ZA, & Pirozzi, D   2017, Technical difficulties and solutions of direct transesterification process of microbial oil for biodiesel synthesis. Biotechnology Letters, 39: 13-23.
Yu, G, Liu, H, Venkateshan, K, Yan, S, Cheng, J, Sun XS & Wang, D 2011, Functional, physiochemical, and rheological properties of duckweed Spirodela polyrhiza protein. Transactions of the ASABE, 54: 555-561.
 Wehr, JD  2003. Freshwater habitats of algae. Freshwater Algae of North America-Ecology and Classification, pp. 11-57. Academic Press, ISBN: 9780123858764
Zhang, D, Gersberg, RM, Ng, WJ & Tan, SK 2014, Removal of pharmaceuticals and personal care products in aquatic plant-based systems: A review, Environmental Pollution, 184: 620-639.