Effects of bacterial isolate, Priestia megaterium HB8 and its filtrate on Controlling the root-knot nematode, Meloidogyne incognita

Document Type : Research Paper

Authors

Department of Plant Protection, Agricultural Engineering Sciences Collage, University of Baghdad, Iraq

10.22124/cjes.2023.6200

Abstract

This study was conducted to isolate biological control agents from soil samples surrounding the roots of uninfected cucumber, watermelon, eggplant, tomato, and okra plants in fields infested with root-knot nematodes and to evaluate their effectiveness. Therefore, fifteen bacterial isolates were selected to study their effects on the vitality of the second stage juveniles (J2) of the root-knot nematode, Meloidogyne incognita. Nine bacterial isolates showed a mortality rate of juveniles ranged between 80 and 100%, while isolate HB8 was unique in having a high ability to influence the vitality of the juveniles, as the mortality rate reached 100%. This isolate was chosen to test its effectiveness in inhibiting egg hatching. The results showed that the inhibition rate after 72 h of incubation reached 94.33%. The effect of the bacterial filtrate of isolate Priestia megaterium HB8 was tested using three concentrations (10, 20, and 30%) on the mortality rate of J2 and the inhibition rate of egg hatching. Moreover, the 30% concentration exhibited a high ability to increase the mortality rate of a juvenile to 97.66% after 48 h of incubation, while the inhibition rate of egg hatching was 96.66% after 72 h.

Keywords

References

Abhilash, M, Shiva Reddy, DM, Mohan, BK, Nataraja, S & Krishnappa, M 2010, Isolation and molecular characterization of Bacillus megaterium isolated from different agro climatic zones of Karnataka and its effect on seed germination and plant growth of Sesamum indicum. Research Journal of Pharmaceutical, Biological and Chemical Sciences, 1: 3.
Al Hazmi, ABS 2009, Introduction to plant nematology. 2nd Edition, King Saud University Press, Riyadh, Saudi Arabia, 440 p.
Borrajo, PM, Mondino, EA, Maronich, GA, Fernandez, M & Creus, CM 2021, Potential of rhizobacteria native to Argentina for the control of Meloidogyne javanica. Revista Argentinel de Microbiology, http: //dol.org/10.1016/j. ram 2021.2.010.
Castaneda Alvarez, C, Prodan, S, Rosales, IM & Aballay, E 2016, Exoenzymes and metabolites related to the nematicidal effect of rhizobacteria on Xiphinema index. Thorne and Allen. Journal of Applied Microbiology, 120: 413-424.
Dameh, M, Ameen Baqer, A, Zaidi, MK, Hachim, SM, Hameed, NA, Awadh, S, Obaid, AJ, Adhab, ZH 2022, Evaluating the efficacy of flavonoids from Capparis spinosa extracts in treatment of Candida vulvovaginitis. Caspian Journal of Environmental Sciences, 20: 565-570
El Hadad, ME, Mustafa, MI, Selim, ShM, Mahgoob, AEA, El Hasan, ST, Inad, DA & Abd Al Rasoul, AM 2020, Diagnostic of new species of root- knot nematode Meloidogyne cruciani associated with eggplant in Babylon governorate, Iraq based on morphological characters and molecular methods. Equal Employment Opportunity Environmental and Conservation, 26: 69 -74.
Hunt, DJ & Handoo ZA 2009, Taxonomy, Identification and principal species. In: RN, Perry, M, Moens JL, Starr (Eds): Root-knot Nematodes. Chapter 3. Wallingford, CAB International: pp. 55-97.
Jepson, SB 1983, The use of second stage juvenile tails as an aid in the identification of Meloidogyne species. Nematologica, 29: 11-28.
Jones, JT, Haegeman, A, Danchin, EGJ, Gaur, HS, Helder, J, Jones, MGK, Kikuchi, T, Manzanilla López, R, Palomares Rius, JE, Wesemael, WM & Perry, RN 2013, Top 10 plant-parasitic nematodes in molecular plant pathology. Molecular Plant Pathology, 14: 946-961
Kepeneki, L, Dura, O & Dura, S 2017, Determination of nematicidal effects of some bio- pesticides against root- knot nematode Meloidogyne incognita on kiwifruit. Journal of Agricultural Science and Technology, 7: 546- 551.
Mahesha, HS, Ravichandra, NG, Rao, MS & Narasegowda, NC 2017, Bio-efficacy of different strains of Bacillus spp. against Meloidogyne incognita under in vitro International. International Journal of Current Microbiology and Applied Sciences, 6: 2511-2517. 
Mendoza, AR, Kiewnick, S & Sikora, RA 2008, In vitro activity of Bacillus firmus against the burrowing nematode Radopholus similis, the root-knot nematode Meloidogyne incognita and the stem nematode Ditylenchus dipsaci. Biocontrol Science and Technology, 18: 377-389.
Mukhtar, T, Kayani, MZ & Hussain, MA 2013, Nematicidal activities of Cannabis sativa L. and Zanthoxylum alatum Roxb. against Meloidogyne incognita.  Industrial Crop Products, 42: 447-453.
Raaijmakers, JM, Paulitz, TC, Steinberg, C, Alabouvette, C & Moënne Loccoz, Y 2009, The rhizosphere: A playground and battlefield for soilborne pathogens and beneficial microorganisms. Plant and Soil, 321: 341-361.
Ralmi, NHAA, Khandaker, MM & Mat, N 2016, Occurrence and control of root knot nematode in crops: A review. Australian Journal of Crop Science, 10: 1649-1654.
Stephan, ZA 1987, Plant parasitic nematodes on cereal and legumes in Iraq. In: Nematodes parasitic to cereal and legumes in Temperate and Semi arid Regions. Proceeding of a workshop held at Larnaca, Cyprus, pp. 155- 159.
Stephan, ZA 1988, Newly reported hosts of root - knot nematodes in Iraq. International Nematology Network Newsletter, 5: 36-43.
Tayeb, TS & Abdel Aziz, NH 2010, In vitro evaluation of some bacterial isolates as biofertilizers and biocontrol agents against the second stage juveniles of Meloidogyne incognita. World Journal of Microbiology and Biotechnology, 26:2249-2256.
Tran, TH, Wang, S, Nguyen, V, Tran, D, Nguyen, D & Nguyen, A 2019, Study of novel endophytic bacteria for biocontrol of black pepper root- knot nematodes in the central highlands of Vietnam. Agronomy, 9: 714.
Vagelas, IK 2015, Novel bacteria species in nematode bio control. In: TH, Askari & PRH Mertinelli (Eds.) Bio control agents of phytonematodes. CABI, Wallingford.
Yadav, U. 2017. Recent trends in nematode management practices: the Indian context. International Journal of Engineering Research & Technology, 4: 482-489.
Youssef, MMA, Abd El Khair, H & El Nagdi WMA 2017, Management of root knot nematode, Meloidogyne incognita infecting sugar beet as affected by certain bacterial and fungal suspensions. Agricultural Engineering International, 9: 293-301.
Caspian Journal of Environmental Sciences
Volume 21, Issue 1
January 2023
Pages 105-114

Files

Share

How to cite

Statistics