Effects of antibiotic residues on some health parameters of Planiliza abu H. in Shatt Al-Arab, Southern Iraq

Document Type : Research Paper

Authors

1 Department of Marine Vertebrates, Marine Sciences Center, University of Basrah, Basrah, Iraq

2 Department of Fisheries and Marine Resources, College of Agriculture, University of Basrah, Basrah, Iraq

10.22124/cjes.2022.6080

Abstract

The presence of antibiotics in the aquatic environment poses great concerns because of their impacts on water quality, aquatic organisms and human health. The current study aimed to detect the residues of antibiotics including amoxicillin (AMO), ciprofloxacin (CIP) and levofloxacin (LEV) seasonally in the muscles and liver of Planiliza abu fish and indicating their effects on some health aspects of fish during November 2020 through August 2021 in two selected stations from Shatt Al-Arab, Basra, Iraq. The samples were analysed using high performance liquid chromatography (HPLC). The current study is the first in Iraq to indicate the presence of antibiotic residues in fish. The study recorded high levels of antibiotics in the muscles and liver of fish, and the concentrations were higher in the second station than in the first one. The antibiotic AMO occupied the highest concentration in the muscles and liver of fish in the second station during the spring exhibiting 8.7 and 6.2 mg kg-1, respectively. The lowest values ​​of CIP were recorded in fish muscles in the first station during the autumn, amounting to 2.8 mg kg-1. The lowest values ​​of LEV were recorded in fish livers in the first station during the autumn amounting to 1.3 mg kg-1. In this study, the accumulation of antibiotics in the liver and muscles of fish exhibited negative effects on the health standards. The presence of antibiotic residues in fish samples in these high concentrations is a source of great concern as it is a major source of human food. The study emphasized the need to conduct more studies to detect this type of pollution and know its negative effects on the health aspects of other types of fish.

Keywords


Abbaszadeh, M, Şişman, T 2021, An application of histological technique for monitoring health status of fish species, Leuciscus aspius (Linnaeus, 1758) inhabiting Aras River, Iran. Caspian Journal of Environmental Sciences, 19: 187-199.
Abdullah, AD, Masih, I, Vvan Dder Zaag, P, Karim, UFA, Popescu, I & Al Suhail, Q 2015, Shatt al Arab River system under escalating pressure: a preliminary exploration of the issues and options for mitigation. International Journal of River Basin Management, 13: 215-227. 
Abedi, Z, Hasantabar, F, Khalesi, MK & Babaei, S 2013, Enzymatic activities in common carp; Cyprinus carpio influenced by sublethal concentrations of cadmium, lead, chromium. World Journal of Fish and Marine Sciences, 5: 144-151.
Akbary, P & Jahanbakhshi, A 2017, Growth yield, survival, carcass quality, haematological, biochemical parameters and innate immune responses in the grey mullet (Mugil cephalus Linneaus, 1758) fingerling induced by Immunogen® prebiotic. Journal of Applied Animal Research, 46: 10-16.
Al Mahmood, HKH, Al Sayaab, HA, Al Miahi, DSB, Mahmoud, AB & Mutasher, WR 2011, One dimensional model to study hydrodynamics properties for north part of Shatt Al Arab River (south Iraq). Basrah Journal of Science, 28: 1-14. https://www.researchgate.net/publication/215449529.
Ben Salem, Z, Capelli, N, Laffray, X, Elise, G, Ayadi, H & Aleya, L 2014, Seasonal variation of heavy metals in water, sediment and roach tissues in a landfill draining system pond (Etueffont, France). Ecological Engineering, 69: 25-37. https://doi:10.1016/j.ecoleng.2014.03.072.
Bielen, A, Simatovic, A, Kosic Vuksic, J, Senta, I, Ahel, M, Babic, S & Udikovic-Kolic, N 2017, Negative environmental impacts of antibiotic-contaminated effluents from pharmaceutical industries. Water Research, 126: 7987, https://doi:10.1016/j.watres.2017.09.019.
Bielen, A, Simatovic, A, Kosic Vuksic, J, Senta, I, Ahel, M, Babic, S & Udikovic Kolic, N 2017, Negative environmental impacts of antibiotic-contaminated effluents from pharmaceutical industries. Water Research, 126: 79–87. https://doi:10.1016/j.watres.2017.09.019.
Bojarski, B & Witeska, M 2020, Blood biomarkers of herbicide, insecticide, and fungicide toxicity to fish - A review. Environmental Science and Pollution Research, 27: 19236–19250, https://doi:10.1007/s11356-020-08248-8
Chen, H, Liu, S, Xu, XR, Diao, ZH, Sun, KF & Hao, QW 2018, Tissue distribution, bioaccumulation characteristics and health risk of antibiotics in cultured fish from a typical aquaculture area. Journal of Hazardous Materials, 343: 140–148. https://doi:10.1016/j.jhazmat.2017.09.017.
Chen, H, Liu, S, Xu, XR, Liu, SS, Zhou, GJ, Sun, KF, Zhao, JL & Ying, GG 2015, Antibiotics in typical marine aquaculture farms surrounding Hailing Island, South China: Occurrence, bioaccumulation and human dietary exposure. Marine Pollution Bulletin, 90: 181-187, https://doi:10.1016/j.marpolbul.2014.10.053.
Cheng, D, Liu, X, Zhao, S, Cui, B, Bai, J & Li, Z 2017, Influence of the natural colloids on the multi-phase distributions of antibiotics in the surface water from the largest lake in North China. Science of the Total Environment, 578: 649–659. https://doi:10.1016/j.scitotenv.2016.11.012.
Coppo, J, Mussart, N & Fioranelli, S 2001, Physiological variation of enzymatic activities in blood of bullfrog, Rana catesbeianna (Shaw, 1802). Revista Veterinaria, 2: 22-27.
Cui, C, Han, Q, Jiang, L, Ma, L, Jin, L, Zhang, D & Zhang, T 2018, Occurrence, distribution, and seasonal variation of antibiotics in an artificial water source reservoir in the Yangtze River delta, East China. Environmental Science and Pollution Research, 25: 19393-19402, https://doi:10.1007/s11356-018-2124.
Fang, L, Chen, X, Shan, X, Qiu, L, Fan, L, Meng, S & Song, C 2021, Antibiotic accumulation, growth performance, intestinal diversification, and function of Nile tilapia (Oreochromis niloticus) feed by diets supplemented with different doses of sulfamethoxazole. Environmental Science and Pollution Researchhttps://doi:10.1007/s11356-021-15253-y.
Gharaei, A, Rayeni, MF, Ghaffari, M, Akrami, R & Ahmadifar, E 2016, Influence of dietary prebiotic mixture (α - mune) on growth performance, haematology and innate immunity of beluga sturgeon (Huso huso) juvenile. International Journal of Aquatic Biology, 4: 277-284.
Gros, M, Petrovi MC & Barcel, DO 2006, Development of a multi residue analytical methodology based on liquid chromatography–tandem mass spectrometry (LC–MS/MS) for screening and trace level determination of pharmaceuticals in surface and waste waters. Talanta, 70: 678-690, https://doi: 10.1016/j. talanta. 2006.05.024.
Hamscher, G, Sczesny, S, Hoper, H & Nau, H 2002, Determination of persistent tetracycline residues in soil fertilized with liquid manure by high-performance liquid chromatography with electrospray ionization tandem mass spectrometry. Analytical Chemistry, 74: 1509-1518, https://doi:10.1021/ac015588m.
Hoseini, SM & Yousefi, M 2018, Beneficial effects of thyme (Thymus vulgaris) extract on oxytetracycline-induced stress response, immunosuppression, oxidative stress and enzymatic changes in rainbow trout (Oncorhynchus mykiss). Aquaculture Nutritionhttps://doi:10.1111/anu.12853.
Hoseini, SM & Yousefi, M 2018, Beneficial effects of thyme (Thymus vulgaris) extract on oxytetracycline-induced stress response, immunosuppression, oxidative stress and enzymatic changes in rainbow trout (Oncorhynchus mykiss). Aquaculture Nutritionhttps://doi:10.1111/anu.12853.
Hu, X, Zhou, Q & Luo, Y 2010, Occurrence and source analysis of typical veterinary antibiotics in manure, soil, vegetables and groundwater from organic vegetable bases, northern China. Environmental Pollution, 158: 2992-2998. https://doi:10.1016/j.envpol.2010.05.023.
Huang, XJ, Choi, YK, Im, HS, Yarimaga, O, Yoon, E & Kim, HS 2006, Aspartate aminotransferase (AST/GOT) and alanine aminotransferase (ALT/GPT) detection techniques. Sensors, 6: 756- 782, https://doi:10.3390/s6070756.
Jorfipour, M, Keivany, Y, Paykan-Heyrati, F, Ghafoori, Z 2022, Feeding indices of Planiliza abu (Heckel, 1843) in Karun River, Southwestern Iran. Caspian Journal of Environmental Sciences, 20: 693-700.
Kemper, N 2008, Veterinary antibiotics in the aquatic and terrestrial environment. Ecological Indicators, 8: 1-13, https://doi:10.1016/j.ecolind.2007.06.002.
Kovalakova, P, Cizmas, L, McDonald, TJ, Marsalek, B, Feng, M & Sharma, VK 2020, Occurrence and toxicity of antibiotics in the aquatic environment: A review.
Li, JY, Wen, J, Chen, Y, Wang, Q & Yin, J 2021, Antibiotics in cultured freshwater products in Eastern China: Occurrence, human health risks, sources, and bioaccumulation potential. Chemosphere, 264: 128441. https://doi:10.1016/j.chemosphere.2020.128441
Limbu, SM, Zhou, L, Sun, SX, Zhang, ML & Du, ZY 2018, Chronic exposure to low environmental concentrations and legal aquaculture doses of antibiotics cause systemic adverse effects in Nile tilapia and provoke differential human health risk. Environment International, 115: 205-219. https://doi:10.1016/j.envint.2018.03.034.
Lu, S, Lin, C, Lei, K, Wang, B, Xin, M, Gu, X, Cao, Y, Liu, X, Ouyang, W & He, M 2020, Occurrence, spatiotemporal variation, and ecological risk of antibiotics in the water of the semi-enclosed urbanized Jiaozhou Bay in eastern China. Water Research, 184: 116187.
Nakano, T, Hayashi, S & Nagamine, N 2015, Effect of excessive doses of oxytetracycline on stress-related biomarker expression in coho salmon. Environmental Science and Pollution Research, 25: 7121-7128, https://doi:10.1007/s11356-015-4898-4.
Naveed, S, Sultana, N, Arayne, MS & Dilshad, HD 2014, A new HPLC method for the assay of levofloxacin and its application in drug-metal interaction studies. Journal of Scientific and Innovative Research, 3: 91-96. https://www.researchgate.net/publication/265164380
Nibamureke, U, Barnhoorn, I & Wagenaar, G 2019, Nevirapine in African surface waters induces liver histopathology in Oreochromis mossambicus: A laboratory exposure study. African Journal of Aquatic Science, 44: 77-88, https://doi:10.2989/16085914.2019.1571477.
Nunes, B, Antunes, SC, Gomes, R, Campos, JC, Braga, MR, Ramos, AS & Correia, AT 2015, Acute effects of tetracycline exposure in the freshwater fish Gambusia holbrooki: antioxidant effects, neurotoxicity and histological alterations. Archives of Environmental Contamination and Toxicology, 68: 371-381, https://doi:10.1007/s00244-014-0101-z
Rajamanickam, V & Muthuswamy, N 2008, Effect of heavy metals induced toxicity on metabolic biomarkers in common carp (Cyprinus Carpio L.). Maejo International Journal of Science and Technology, 2: 192-200.
Robert, KM, Victor, WR, David, B, Kathleen, MB, Anthony, PW & Peter, JK 2011, Harpers illustrated biochemistry (Lange Medical Book), 28th Edition, 28, McGraw-Hill.
Thakare, R, Kesharwani, P, Dasgupta, A, Srinivas, N & Chopra, S 2020, Chapter 1 – Antibiotics: past, present, and future. In: Kesharwani, P., Chopra, S., Dasgupta, A. (Eds.), Drug Discovery Targeting Drug-Resistant Bacteria. Academic Press, pp. 1-8, https://doi.org/10.1016/B978-0-12-818480-6.00001-1.
Torres, NH, De Salles Pupo, MM, Ferreira, LFR, Maranho, LA, Americo Pinheiro, JHP, Vilca, FZ & Tornisielo, VL 2017, Spatial and seasonal analysis of antimicrobials and toxicity tests with Daphnia magna, on the sub-basin of Piracicaba river, SP, Brazil. Journal of Environmental Chemical Engineering, 5: 428 6070-6076. https://doi.org/10.1016/j.jece.2017.11.031.
Unutkan, T, Bakırdere, S & Keyf, S 2018, Development of an analytical method for the determination of amoxicillin in commercial drugs and wastewater Samples, and assessing its stability in simulated gastric digestion. Journal of Chromatographic Science, 56: 36-40, https://doi.org/10.1093/chromsci/bmx078.
Zhang, B, Xu, L, Hu, Q, Zeng, X & Yu, Z 2020, Occurrence, spatiotemporal distribution and potential ecological risks of antibiotics in Dongting Lake, China. Environmental Monitoring and Assessment, 192. https://doi:10.1007/s10661-020-08761-w.