Nymphicidal and adulticidal action of Beauveria bassiana isolates in whitefly Bemisia tabaci (Hemiptera: Aleyrodidae) in vitro

Document Type : Research Paper

Authors

College of Education for Pure Sciences, Wasit University, IRAQ

10.22124/cjes.2022.6050

Abstract

Whitefly Bemisia tabaci is one of the most dangerous and destructive pests for crops in fields and greenhouses. Entomopathogenic fungi have emerged as an effective management method compared to potential disorders created by chemical pesticides, including environmental pollution and development of resistance. Therefore, the bio- control agents (microbial pesticides) provide an alternative to chemical pesticides due to their cheapness and ease of handling with their safe use for farmers and more selective than chemical pesticides. The laboratory study objective was to evaluate the efficiency of the bio-control agent (Beauveria bassiana) in the control of the nymph and adult stages of B. tabaci on cucumber crop. Three concentrations of fungal filtrate of this fungus, 0.25, 0.50, 1.00% conidia mL-1 were used in this study. The results showed high virulent of two tested isolates against the nymph and adult stages of the B. tabaci that different significantly when compared to the control. The mortality rate was increased by elevating the concentration and by increasing in time period of the nymph and adult exposure to fungal filtrates. The 100% concentration for both two isolates (Bb100 and Bb90) was superior from the rest of the concentrations and exhibited the mortality rate in the nymph and adult stages of this pest as 55.55% and 55.55% respectively in nymphs stage, while 52.22% and 51.10% in adult stages. In addition, in the case of effects of time periods on mortality rate after treatment, the highest mortality rate of nymphs occurred after 9 days of treatment, amounting to 84.44% and 66.66% for Bb100 and Bb90 in nymph stage, while 68.88% and 65.55% in adult stage respectively. From the point view of interaction between concentrations and time periods, the highest mortality rate in nymphs was recorded at the concentration of 1.00% after 9 days of treatment amounting to 96.66%, 93.33% for Bb100 and Bb90 respectively and 83.33%, 86.66% in adults respectively. The lowest mortality rate in nymph was 6.66 after 3 days of treatment at the concentration of 0.25% for both isolates in nymph, while in adults were 13.33% and 10.0% for Bb100 and Bb90 respectively.  

Keywords

References

Abbas, KM & Al-Rahmanny, AHJ 2022, Toxic effects of various oil concentrations obtained from Rosmarinus officinalis on Musca domestica adults (Diptera: Muscidae) in different time periods. Caspian Journal of Environmental Sciences, 20: 401- 405.
Ahmed, F, Anwar, W, Javed, MA, Basit, R, Akhter, A, Ali, S, Khan, HAA, Amin, H & Haider, MH 2019, Infection mechanism of Aspergillus and Fusarium species against Bemisia tabaci. Mycopath, 17: 69-78.
Ahmad, F, Anwar, W, Javed, MA, Basit, R, Akhter, A, Ali, S, Ali Khan, HA, Amin, H & Haider, MA 2019, Infection mechanism of Aspergillus and Fusarium species against Bemisia tabaci. Mycopath, 17: 69-78.                                                                                                    
Ahmed, M, Ghazal, I, Kerhili, S & Rajab, L 2018, The pathogenicity of the fungus Beauveria bassiana (Bals.) Vuil. on adults and eggs of the two spotted spider mite Tetranychus urticae Koch in the laboratory. Arab Journal of Plant Protection, 36: 199-206.                                                                     
AL Jubouri, IJ, Abdul Sattar, AA & Al Anbaki, NN 2000, Cotton pests and her control methods, national program for the development of cotton cultivation in Iraq. Indicative Bulletin, 60 p.
Almuhsin Ahmed, IA, Al-Jboori, MJ & AlBahadly, ZK 2022, The double infection diagnosis, fungal and insect on date palm offshoot Phoenix dactylifera with treatment pesticides and practical technique. Caspian Journal of Environmental Sciences, 20: 855- 858.
Al Rabiei, HAA & Salami, WM 2016, The effect of some isolated fungal suspensions of Tetranychus urticae Koch in her different life stages. Al-Furat Journal of Agricultural Sciences, 8: 151-8.  
  Alwan, SH 2022, The toxic impact of the extract of the Dieffenbachia picta leaves on the ratio of death in the termites' workers Microcerotermes diversus (silvestri) [Isoptera: Termitidae]. Caspian Journal of Environmental Sciences, 20: 217- 220.                                    
Al Zubaidi, ANA, Al Salami, WM & Naas, HAJ 2010, Effect of different concentrations of the fungal filtrate of the fungus Aspergillus niger in nymphs and adults of the whitefly Bemisia tabaci Genn. (Homoptea: Aleyroiddae). Al-Furat Journal of Agricultural Sciences, 2: 176-182.                                                                                                   
Anjum, F & Wright, D 2016, Relative toxicity of insecticides to the crucifer pests Plutella xylostella and Myzus persicae and their natural enemies. Crop Protection, 88: 131-136.                                                                                  
Anwar, W, Javed, MA, Shahid, AA, Nawaz, K, Akhter, A, Ur Rehman, MZ, Hameed Iftikhar, S & Haider, MS 2019, Chitinase genes from Metarhizium anisopliae for the control of whitefly in cotton. Royal Society Open Science, 6: 190412.
Bugti, GhA, Bin, W, Na, C & Feng, LH 2018, Pathogenicity of Beauveria bassiana Strain 202 against Sap-sucking Insect Pests. Plant Protection Science, 54: 111-117.                                             
Cabanillas, HE & Jones, WA 2009, Pathogenicity of Isaria sp. (Hypocreales: Clavicipitaceae) against the sweet potato whitefly B biotype. Bemisia tabaci (Hemiptera: Aleyrodidae). Crop Protection, 28: 333-337
Choudhary, H, Parihar, S, Singh, S & Parvez, N 2017, Technical Bulletin on Whiteflies. H, Choudhary, S, Parihar, S, Singh, N, Parvez, Eds., National Innovation Foundation-India: Gujarat, India                                                  
Cuthbertson, AGS 2013, Update on the status of Bemisia tabaci in the UK and the use of entomopathogenic fungi within eradication programmes. Insects, 4: 198-205.                                                                                                     
De la Rosa, W, López, FL & Liedo, P 2002, Beauveria bassiana as a pathogen of the Mexican fruit fly (Diptera: Tephritidae) under laboratory conditions. Journal of Economic Entomology, 95: 36-43. ‏
Elhakim, E, Mohamed, O & Elazouni, I 2020, Virulence and proteolytic activity of entomopathogenic fungi against the two-spotted spider mite, Tetranychus urticae Koch (Acari: Tetranychidae). Egyptian Journal of Biological Pest Control, 30: 30.                                                                              
Faria, M & Wraight, SP 2001, Biological control of Bemisia tabaci with fungi. Crop Protection, 20: 767-778.                      
 Flore, Z, Mustafa, A, Inês, S, Ibrahim, C & Sara, M 2019, Inter-and intra-specific variation of spider mite susceptibility to fungal infections: implications for the long-term success of biological control. CC-BYNC-ND4.0 International License It, pp 1-19.                                                                           
Fortes, IM, Sánchez Campos, S, Fiallo Olivé, E, DíazPendón, JA, Navas Castillo, J & Moriones, E 2016, A novel strain of tomato leaf curl New Delhi virus has spread to the Mediterranean basin. Viruses, 8: 307-325.                                    
Gao, T.; Wang, Z.; Huang, Y.; Keyhani, N.O.; Huang, Z (2017). Lack of resistance development in Bemisia tabaci to Isaria fumosorosea after multiple generations of selection. Scientific Reports, 7: 1-11.                                                        
Garcia, AS, Messias, CL, De Souza, HML & Piedramuena, AE 1984, Pathogenicity of   Metarhizium anisopliae var.  anisopliae a Ceratitis capitata Wied) (Diptera: Tephritidae). Revista Brasileira de Entomologia, 28: 421-424.
Geroh, M, Gulati, R & Tehri, K 2014, Beauveria bassiana (balsamo Vuillemin (Strain ITCC- 4668) as acaricide against Tetranychus urticae Koch Acari: Tetranychidae). Indian Journal of Agricultural Research, 48: 384-388.                
 Kanika, T, Rachna, G, Monika, G & Dhankhar, SK 2015, Dry weather: A crucial constraint in the field efficacy of entomopathogenic fungus Beauveria Bassiana against Tetranychus urticae Koch (Acari: Tetranychidae). Journal of Entomology and Zoology Studies, 3: 287-291.                                                
Lacey, LA, Wright, SP & Kirk, AA 2008, Entomopathogenic fungi for control of Bemisia tabaci biotype B: foreign exploration, research and implementation. In: J, Gould, K., Hoelmer & J, Goolsby (Eds.), Classical biological control of Bemisia tabaci in the United States: A review of interagency research and implementation. Progress in Biological Control, 4: 33–69.
Li, N, Li, Y, Zhang, S, Fan, Y & Liu, T 2017, Effect of elevated CO2 concentration and temperature on antioxidant capabilities of multiple generations of Bemisia tabaci MEAM1 (Hemiptera: Aleyrodidae). Journal of Insect Physiology, 103: 91-97.                                                                              
Maan, A 2017, Test of vitality of fungus Beauveria bassiana (Bals.) Vuill. on eggs and larvae of moth Figs Ephestia cautella (Walk.) (Lepidoptera: Pyralidae). Al-Mustansiriyah Journal of Science, 28.                    
Manal, ARAMAR & Hany, MH & Heikal, M 2019, Toxicity of some pesticides and plant extracts on Tetranychus urticae and its Predator, Phytoseiulus persimilis. International Journal of Zoological Research. 5: 73-82.                                                                                                                  
Mascarin, GM, Kobori, NN, Quintela, ED & Ju´nior, ID 2013, The virulence of entomopathogenic fungi against Bemisia tabaci biotype B (Hemiptera: Aleyrodidae) and their conidial production using solid substrate fermentation. Biological Control, 66: 209-218.
Negash, R, Dawd, M & Azerefegne, F 2014, Pathogenicity of Beauveria bassiana and Metarhizium anisopliae, to the two spotted spider mites, Tetranychus urticae (Acari: Tetranychidae) at different temperatures and under greenhouse conditions. Ethiopian Journal of Agricultural Sciences, 24: 51-58. 
Ortucu, S & Iskender, NA 2017, Determination of control potentials and enzyme activation of Beauveria bassiana (Bals.) Vuil. isolates against Tetranychus urticae Koch (Acari: Tetranychidae). Trakya University Journal of Nature Sciences, 18: 33-38.                                                                              
Osborn, LS, Hoelmer, K & Gerling, D 1990, Prospects for biological, damage, control and management. Audorer, UK Intercept, 702 p.               
Perring, TM, Stansly, PA, Liu, TX & Smith, HA & Andreason, SA 2018, Whiteflies: Biology, ecology, and management. In: Sustainable management of arthropod pests of tomato; W, Wakil, GE, Brust, TM, Perring (Eds.), Academic Press: Cambridge, MA, USA, Elsevier: Amsterdam, The Netherlands, pp. 73-110.                                                                      
Petlamul, W & Prasertsan, P 2012, Evaluation of strains of Metarhizium anisopliae and Beauveria bassiana against Spodoptera litura on the basis of their virulence, germination rate, conidia production, radial growth and enzyme activity. Mycobiology, 40: 111-116.
Reay, DS, Hollingworth, KA, Williams, GE, Crozier, FE, Jamieson, DY & Beedell, PL 2008, A darker shade of pale: whiteness, the middle classes and multi-ethnic inner city schooling. Sociology, 41: 1041-1060.
Sabrine, A, Kaouthar, GL, Stephanie, H, Georges, L, Thierry, H 2015, An analysis of potential resistance of the phytophagous mite, Tetranychus urticae Koch (Acari: Tetranychidae) to four botanical pesticides. Biotechnology, Agronomy and Society and Environment, 19: 232-238. 
Salehi, M, Ghods khah Daryaei, M, Amanzadeh, B, Mousavi Koper, S.A 2021, Antixenosis resistance of one-year-old poplar seedlings of different clones to poplar clearwing moth, Paranthrene tabaniformis Rott. (Lep: Sessiidae). Caspian Journal of Environmental Sciences, 19: 415-422                                           
Sanaa, SA, Alaa, JS & Yahya, AS 2020, The effects of biological and chemical agents on the management of main pests in tomato plant. Al-Qadisiyah Journal For Agriculture Sciences (QJAS), ISSN: 2618-1479, 10: 325-334.                                                               
Serkan, O & Nurcan, AI 2017, Determination of control potentials and enzyme activities of Beauveria bassiana (bals.) vull. isolates against Tetranychus urticae Koch (Acari: tetranychidae). Trakya University Journal of Natural Sciences, 18: 33-38.                                                                          
Siddhapara, MR 2015, Biology, seasonal incidence and management of red spider mite, Tetranychus urticae Koch in okra, MSc. Dissertation, Department of Entomology, College of Agriculture, Junagadh Agricultural University Junagadh, India, No. 362 01, pp. 32-33.
Singh, G & Prakash, S 2010, Fungi Beauveria bassiana (Balsamo) metabolites for controlling malaria and filarial in tropical countries. Advanced Biomedical Research, 9: 238-242.                                                                                  
Somoza Vargas, CE, Hernández Velázquez, VM, Peña Chora, G, Torres García, G, Huerta DeLa Peña, A, Ortega Martínez, LD & Salazar Magallón, GA 2018, Interaction of Beauveria bassiana strain HPI-019/14 and Bacillus thuringiensis strain GP 139 for the biological control of Bemisia tabaci in strawberry. Bulletin of Insectology, 71: 201-209.
Tehri, K 2014, A review on reproductive strategies in two spotted spider mite, Tetranychus urticae Koch (Acari: Tetranychidae). Journal of Entomology and Zoology Studies, 2: 35-39.                                                                                                
 Ullah, MS & Lim, UT 2015, Laboratory bioassay of Beauveria bassiana against Tetranychus urticae (Acari: Tetranychidae) on leaf discs and potted bean plants. Experimental and Applied Acarology, 65: 307–318.                              
Xu, C, Qiu, B, Cuthbertson, AG, Zhang, Y & Ren S 2012, Adaptability of sweet-potato whitefly Bemisia tabaci (Hemiptera: Aleyrodidae) on seven marginal host plants. International Journal of Pest Management, 58: 297-301.                                                  
Yesilayer, A 2018, Efficiency of two different entomopathogen fungi Beauveria bassiana and Purpureocillium lilacinum TR1 against Tetranychus urticae. Applied Ecology and Environmental Research, 16: 6077-6086.            
Zhang, C, Shao, ZF, Han, YY, Wang, XM, Wang, ZQ, Musa, PD, Qiu, BL & Ali, S 2018, Effects of Aschersonia aleyrodis on the life table and demographic parameters of Bemisia tabaci. Journal of Integrative Agriculture, 17: 389-396.                               
Caspian Journal of Environmental Sciences
Volume 20, Issue 5
Special Issue: Economical Aspects of Environmental Studies
December 2022
Pages 967-975

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