Molecular study of the genus Eryngium L. (Apiaceae) in Iraq

Authors

1 Department of Ecology, College of Science, University of Kufa, Najaf, Iraq

2 Department of Biology, College of Education, University of Salahaddin, Erbil, Erbil, Iraq

Abstract

This study was conducted to investigate the complete sequences of nuclear ITS region, which determined five species of Eryngium L. Apiaceae in Iraq to inference phylogenetic relationships. Pycnocycla aucheriana was used as outgroups. Phylogenetic analysis was performed using ClastalW in MEGA6 version 7.0.4.1 with high supported (bootstrapping) value for each clade in a dendrogram. The phylogenetic trees showed Eryngium glomerutum as the basal lineage in a dendrogram. The sister clade to E. glomerutum was E. billardieri which form the second clade in phylogenetic trees. The third clade included only E. creticum which is considered as a sister clade to the E. campestre and E. thyrosoideum in the dendrogram. Finally, the monophylly of each clade was well supported, and phylogenetic relationships between Enyngium species was cleared based on DNA Sequencing character. 

Keywords

References

Ahmad, SA 2013, Vascular Plants of Hawraman Region in Kurdistan Iraq.  PhD Dissertation, University of Sulaimani, Sulaimaniya, Iraq, pp. 28-29.
Al- Abbasi, SHAH, Altaef, AQ, AL-Samarraie, M, Al-Naqib, ATH, Al-Majmaei, AAM 2022, Collection and diagnosis of some wild plants in the vicinity of Samarra University, Iraq. Caspian Journal of Environmental Sciences, 20: 437-440.
Aliyeva, G, Ojaghi, J, Rustamova, S 2021, Molecular diversity and phylogenetic analysis of Azerbaijan oaks (Quercus spp.) revealed by RAPD markers. Caspian Journal of Environmental Sciences, 19: 457-468.
Al Rawi, A 1964, Wild plants of Iraq with their distribution. State Board for Agricultural & Water Resources Research, National Herbarium of Iraq, Ministry of Agriculture & Irrigation, Baghdad, pp. 88-97.
Baiba, I, Naeem, HS, Andrew, JF, Gederts, I & Nils, R 2010, Development of retrotransposon-based SSAP molecular marker system for the study of genetic diversity in sea holly (Eryngium maritimum L). Plant Genetic Resources, 8: 258–266.
Carolina, IC, Susana, GM & Stephen, RD 2008, The evolutionary history of Eryngium (Apiaceae, Saniculoideae): Rapid radiations, long-distance dispersals, and hybridizations. Molecular Phylogenetics and Evolution, 46: 1129-1150.
Carolina, IC, Susana, GM & Stephen, RD 2010, Unravelling the taxonomic complexity of Eryngium L. (Apiaceae, Saniculoideae): Phylogenetic analysis of 11 non-coding cpDNA loci corroborates rapid radiations. Plant Diversity and Evolution, 128: 137-149.
Chakravarty, HL 1976, Plant wealth of Iraq. A Dictionary of Economic Plants, Vol. 1, Botany Directorate, Ministry of Agriculture & Agrarian Reform, Baghdad, Iraq.
Chater, AO 1968, In Flora Europaea.Vol.2. Cambridge University Press, pp. 320-324.   
Dana, J, Al Faoury, H, Ayyoubi, Z & Al Safadi, B 2010, A molecular and ecological study of Eryngium species in Syria. Biologia, 65: 796-804.
Darwesh, DTD 2017, Plant Biodiversity and Ethnobotanical Properties of Various Plants in Choman (Erbil-Iraq). M.  Sc.  Thesis, Kahramanmaraș Sütçü İmam University, Graduate School of Natural and Applied Sciences University, Kahramanmaraș, Turkey: 1
Davis, PH 1972, In: Flora of Turkey. Vol. 4. Edinburgh at the University Press, pp. 292-304.
Doyle, JJ & Doyle, JL 1990), Isolation of plant DNA from fresh tissue. Focus, 12: 13-15.
Faris, YS 1983, The vascular plants of Pira Magrun Mountain. MSc. Dissertation, Salahaddin University, Erbil, Iraq, 111 p.
Fatah, HU 2003, The vascular plants of Haibat Sultan Mountain and the adjacent areas. MSc. Dissertation, University of Sulaimani, Sulaimaniya, Iraq, pp. 96-97.
Ghahreman, A & Attar, F 1999, Biodiversity of plant species in Iran. Central Herbarium, Tehran University, Tehran, Iran, 121 p.
Ghazanfer, ShA & Edmondson, JR 2013, Flora of Iraq. Vol. 5, Part 2, Royal Botanic Gardens, KEW, Ministry of Agriculture, Iraq, pp. 113-119.
Hall, RE 2001, The stock market and capital accumulation. American Economic Review, 91: 1185-1202.
Hameed, MAH 2016, Vascular plant taxa of Hujran Basin, Erbil, Iraq. MSc. Dissertation, Kahramanmaraș Sütçü İmam University, Graduate School of Natural and Applied Sciences University, Kahramanmaraș, Turkey, pp. 59- 62
Hasan, HH 2019, A comparative systematic study of the genus Potentilla L. (Rosaceae) in Kurdistan Region, Iraq. MSc. Dissertation, University of Salahuddin -Erbil, Iraq.
Khalaf, MK 1980, The vascular plants of Jabal Sinjar. MSc. Dissertation, Baghdad University, Baghdad, Iraq, 149 p.
Migahid, AM & Hammouda, MA 1978, Flora of Saudi Arabia. Vol. 1, Riyadh University Publication, pp. 375-386.
Milani, E, Saeidi Mehrvarz, Sh & Gholizadeh, H 2017, Floristic, life form and chorological studies of the Abshar protected area, Shirgah, Mazandaran Province, north of Iran Caspian Journal of Environmental Sciences, 15: 165-180.
Rechinger, KH 1964, Flora of low land Iraq. Weinheim J. Cramer, OCLC Number: 912422, pp. 446-457.
Ridda, TJ & Daood, WH 1982, Geographical distribution of wild vascular plants of Iraq. National Herbarium of Iraq, 78 p.
Singh, G 2010, Plant systematics, An integrated approach. 3rd Edition, Science Publishers, Enfield, NH, USA, 644 p.
Swofford, DL 2000, PAUP:  Phylogenetic Analysis Using Parsimony, Version 4, Sunderland, Massachusetts, Sinauer Associates, pp. 6-10.
White, TJ, Bruns, T, Lee, SJ & Taylor, W 1990, Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetic. In: MA, Innis, DH, Gelfand, JJ, Sninsky & TJ White (Eds.) PCR protocols: A guide to methods and applications. Academic Press, New York, NY, USA, pp. 317.
Caspian Journal of Environmental Sciences
Volume 20, Issue 4
October 2022
Pages 845-848

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