Genotypic and environmental variability of the total content of tocopherols in the seeds of inbred sunflower lines

Document Type : Research Paper

Authors

V.S. Pustovoit All-Russian Research Institute of Oil Crops, 17 Filatova street, Krasnodar, 350038, Russia

Abstract

Breeding for high total content of tocopherols (vitamin E) in sunflower seed oil involves studying the genetics of this trait. Twenty-nine inbred lines of sunflower with different total tocopherol content (TTC) were selected from 60 samples of the original material and studied at VNIIMK, Krasnodar, in 2016-2019. TTC was determined in an average sample from a head of shelled seeds by direct hexane extraction and further measurement of the optical density of the colored solution on a spectrophotometer. Both the genotype and the growing conditions influence the TTC in the seeds. The average range of variation over four years was from 302 to 605 mg kg-1. The largest differences, 2.6 times, between genotypes were found in 2018 with a range of variability from 258 to 667 mg kg-1. Significant differences were found between the average values of 360 mg kg-1 in 2017 and 432 mg kg-1 in 2019. The genotype and the year of growing have the greatest influence on the TTC, measured as 65% and 8%, respectively, and the genotype-year interaction accounts for 14% of the effect. The TTC correlation coefficient in the parent-offspring series was 0.65–0.85, and the heritability coefficient in the studied lines was 42–72%. The data on the valuable genetic control of TTC in seeds can help predict the effectiveness of breeding to improve this trait.

Keywords


Alpaslan, M & Gunduz, H 2000, The effects of growing conditions on oil content, fatty acid composition and tocopherol content of some sunflower varieties produced in Turkey. Nahrung, 44: 434-437.
Chen, S, Li, H & Liu, G 2006, Progress of vitamin E metabolic engineering in plants. Transgenic Research, 15: 655-665.
Del Moral L, Pérez-Vich, B, Fernández-Martínez, JM & Velasco, L 2011, Inheritance of increased seed tocopherol content in sunflower line IAST-413. Plant Breeding, 130: 540-543.
Del Moral L, Pérez-Vich, B, Fernández-Martinez, JM & Velasco, L 2011, Inheritance of deficient tocopherol accumulation in sunflower seeds. Journal of Genetics, 90: 489-491.
Del Moral L, Fernández-Martínez, JM, Pérez-Vich, B & Velasco, L 2013, Accumulation dynamics of seed tocopherols in sunflower lines with modified tocopherol levels. Acta Physiologiae Plantarum, 35: 3157-3165.
DellaPenna, D & Pogson, BJ 2006, Vitamin synthesis in plants: Tocopherols and carotenoids. Annual Review of Plant Biology, 57: 711-738.
Demurin, YN 1986, Phenotypic variability and correlation of the content of tocopherols with some biochemical characteristics of sunflower seeds. NTB VNIIMK, 93: 21-24, [in Russian].
Demurin, YN, Peretiagina, TM & Gordovskaia, NN 2018, Variability of the content of tocopherols in the seeds of sunflower lines. Oil crops, pp. 20-22, [in Russian].
Dong, G, Xiaoli, L, Zhongyue, C, Weidong, P, Hongjie, L & Gongshe, L 2007, The dynamics of tocopherol and the effect of high temperature in developing sunflower (Helianthus annuus L.) embryo. Food Chemistry, 102: 138-145.
Falk, J & Munné-Bosch, S 2010, Tocochromanol functions in plants: Antioxidation and beyond. Journal of Experimental Botany, 61: 1549-1566.
Hussain, N, Irshad, F, Jabeen, Z, Shamsi, I & Jiang, L 2013, Correlations between tocopherol and fatty acid components in germplasm collections of Brassica oilseeds. Journal of Agricultural and Food Chemistry, 61: 6137-6149.
Kamal-Eldin, A & Appelqvist, LA 1996, The chemistry and antioxidant properties of tocopherols and tocotrienols. Lipids, 31: 671-701.
Marquard, R 1990, Untersuchungenuberdemeinflussvonsorte und standort auf den tocopherol gehaltverschied enerpflanzenole. Fat Science and Technology, 92: 452-455
Munné-Bosch, S 2005, The role of alpha-tocopherol in plant stress tolerance. Journal of Plant Physiology, 162: 743-748.
Mène-Saffrané, L 2018, Vitamin E biosynthesis and its regulation in plants. Antioxidants, 7: 2.
Mustacich, DJ, Bruno, RS & Traber, MG 2007, Vitamin E. Vitamins & Hormones, 76: 1-21.
Padley, FB, Gunstone, FD & Harwood, JL 1994, Occurrence and characteristics of oils and fats. The Lipid Handbook, Chapman & Hall, London, pp. 47-223.
Rokitskii, PF 1978, Introduction to statistical genetics. Minsk: Vysshaia shkola, 448 p, [in Russian].
Somerville, C, Browse, J, Jaworski, JG & Ohlrogge, JB 2000, Lipids, biochemistry and molecular biology of plants, American Society of Plant Physiology, Rockville, Maryland, USA, p. 456-527.
Tasan, M & Demirci, M 2005, Total and individual tocopherol contents of sunflower oil at different steps of refining. European Food Research and Technology, 220: 251-254.
Velasco, L, Del Moral L, Perez-Vich, B & Fer-nandez-Martinez, JM 2010, Selection for contrasting seed tocopherol content in sunflower seeds. Journal of Agricultural Science, 148: 393-400.
Velascol, L, Fernandez-Martinez, JM, Garcia-Ruiz, R & Dominguez, J 2002, Genetic and environmental variation for tocopherol content and composition in sunflower commercial hybrids. Journal of Agricultural Science, 139:425-429.
Wen, Y, Xu, L, Xue, C, Jiang, X & Wei, Z 2020, Assessing the impact of oil types and grades on tocopherol and tocotrienol contents in vegetable oils with chemometric methods. Molecules, V. 25, 5076.
Zhirong, L, Keasling, J & Niyogi, K 2012, Overlapping photoprotective of vitamin E and carotenoids in Chlamydomonas. Plant Physiology, 158: 313-323.