Evaluating the biological activities of biosynthesized ZnO nanoparticles using Escherichia coli

Document Type : Research Paper

Authors

Department of Biology, College of Science, Mustansiriyah University, Iraq

Abstract

About 50 isolates (83%) of Escherichia coli were identified from 60 stool sample, and 30 examined bacteria formed biofilm. ZnO NPs was synthesized by E. coli and a white cluster pellet appeared, followed by observing absorption peak of UV-Vis. spectroscopy at 268 nm. XRD pattern showed the lattice planes of 100, 002, 101, 102, 110, 103 and 112 compatible to the 2θ values of 32.45°, 34.73°, 36.56°, 47.70°, 55.86°, 62.12°and 63.10° respectively, and the diffraction peaks were assigned with the hexagonal phase, while SEM images exhibited that size of the particles ranged between 31.55-45.9 nm. ZnO NPs displayed antibacterial potentiality against pathogenic bacteria, and inhibition zones around ZnO NPs were as follows: 5, 4, 2, 2, and 2 mm for Pseudomonas aeruginosa, Staphylococcus aureus, Acinetobacter baumannii, Klebsiella pneumoniae, and E. coli respectively. In addition, ZnO NPs was able to decrease biofilm, revealing that after 48 h of incubation, inhibition percentage were 18.6, 27.7, 39.4, and 19.6 % against S. aureus, P. aeruginosa, A. baumannii, and K. pneumoniae, respectively. A549 cells viability was decreased by elevating the concentration of ZnO NPs, and the IC50 values of the A549 and WRL cells were 105.8 and 167.3 µg mL-1 respectively. In this study, the synthesized ZnO NPs using nonpathogenic E.coli showed antibacterial, antibiofilm and anticancer activities against the examined pathogenic bacteria. So, these nanoparticles can be further used in biomedical, pharmaceutical and other applications as an effective antimicrobial and anti-cancerous agent. 

Keywords


Abd Ulkareem, AO 2012, Prevention of Proteus mirabilis biofilm by surfactant solution. Egyptian Academic Journal of Biological Sciences, G. Microbiology, 4: 1-8.‏
Alaskaree, AA 2018, Biosynthesis of Zinc Oxide nanoparticles (ZnO NPs) by probiotic bacteria and their effect on bacterial skin infections. (PhD Thesis). Collage of Science, Mustansyriah University.
Antony, AC, Mini, KP, Reshma, S, Aneesa, PA, Suresh, K, Divya, PS, Simmy, P, Fathima PA, and Mohamed, HA 2016, Comparative evaluation of EMB agar and hicrome E. coli agar for differentiation of green metallic sheen producing non E. coli and typical E. coli colonies from food and environmental sample. Journal of Pure and Applied Microbiology, 10(4), DOI: http://dx.doi.org/10.22207/JPAM.10.4.48.
Babapour, E 2016, Biofilm formation in clinical isolates of nosocomial Acinetobacter baumannii and its relationship with multidrug resistance. Asian Pacific Journal of Tropical Biomedicine, 6: 528-533.‏
Bagherzadeh Lakani, F, Meshkini, S, Yazdani Sadati, MA, Falahatkar, B  2016, Bioaccumulation of copper nanoparticle in gill, liver, intestine and muscle of Siberian sturgeon (Acipenser baerii) juvenile. Caspian Journal of Environmental Sciences, 14: 105-115.
Bozorgpanah Kharat, Z, Mohammadi Galangash, M, Ghavidast, A, Shirzad-Siboni, M 2018, Removal of reactive black 5 dye from aqueous solutions by Fe3O4@SiO2-APTES nanoparticles. Caspian Journal of Environmental Sciences, 16: 287-301.
Chandrasekaran, M & Pandurangan M 2016, In vitro selective anti-proliferative effect of zinc oxide nanoparticles against co-cultured C2C12 myoblastoma cancer and 3T3-L1 normal cells. Biological trace element research, 172: 148-154, ‏ DOI: 10.1007/s12011-015-0562-6. Epub 2015 Nov 12.
Daneshvar, N 2008, Preparation and investigation of photocatalytic properties of ZnO nanocrystals: Effect of operational parameters and kinetic study. Evaluation, 900.6,‏ DOI: doi.org/10.5281/zenodo.1072778.
FDA 2015, Select Committee on GRAS Substances (SCOGS) Opinion: Tannic acid (hydrolyzable gallotannins). GRAS substances (SCOGS) database.
Hu, Y, Chen, HJ 2008, Preparation and characterization of nanocrystalline ZnO particles from a hydrothermal process. Journal of Nanoparticle Research, 10: 401-407.‏
Hasan, ZH 2016, Inhibition of Biofilm formation by silver nanoparticles biosynthesized by pathogenic Escherichia coli., MSc. Dissertatio,. Collage of Scinence, Mustansyriah University.
Duarte, A 2016, Clinical isolates of Acinetobacter baumannii from a Portuguese hospital: PFGE characterization, antibiotic susceptibility and biofilm-forming ability. Comparative immunology, microbiology and infectious diseases, 45: 29-33.‏
Ifeanyichukwu, UL, Fayemi, OE, Ateba, CN 2020, Green synthesis of zinc oxide nanoparticles from pomegranate (Punica granatum) extracts and characterization of their antibacterial activity. Molecules, 25: 4521.‏
Jayaseelan, C, Rahuman, AA, Kirthi, AV, Marimuthu, S, Santhoshkumar, T, Bagavan, A, Gaurav, K, Karthik L &
Karigoudar Rashmi, M 2019, Detection of biofilm among uropathogenic Escherichia coli and its correlation with antibiotic resistance pattern. Journal of laboratory physicians, 2019, 11.1: 17.‏
Johari, SA, Asghari, S, Yu, IJ 2016a, Toxicity of various silver nanoparticles compared to silver ions in the Ponto-Caspian amphipod Pontogammarus maeoticus (Sowinsky, 1894).  Caspian Journal of Environmental Sciences, 14: 25-32.
Johari, SA, Asghari, S, Barsch, N 2016b, Toxicity comparison of silver nanoparticles synthesized by physical and chemical methods to tadpole (Rana ridibunda). Caspian Journal of Environmental Sciences, 14: 383-390.
Khan, MR et al. 2020, Metal nanoparticle–microbe interactions: Synthesis and antimicrobial effects. Particle & Particle Systems Characterization, 37.5: 1900419.‏
Martínez-carmona, M, Gun’ko, Y & Vallet-regí, M 2018, ZnO nanostructures for drug delivery and theranostic applications. Nanomaterials, 8: 268,‏ DOI: 10.3390/nano8040268.
Miyaue, S et al. 2018, Bacterial memory of persisters: Bacterial persister cells can retain their phenotype for days or weeks after withdrawal from colony–biofilm culture. Frontiers in microbiology, 9: 1396.
Muhammad, W 2019, Optical, morphological and biological analysis of zinc oxide nanoparticles (ZnO NPs) using Papaver somniferum L. RSC advances, 9: 29541-29548.‏
Murugan, N 2016, Unraveling genomic and phenotypic nature of multidrug-resistant (MDR) Pseudomonas aeruginosa VRFPA04 isolated from keratitis patient. Microbiological Research, 193: 140-149.‏
‏ Namasivayam, S & Karthick R 2013, Anti-biofilm effect of biogenic silver nanoparticles coated medical devices against biofilm of clinical isolate of Staphylococcus aureus. Global Journal of Medical Research, 13: 1-7.‏
 Nirwati, H 2019, Biofilm formation and antibiotic resistance of Klebsiella pneumoniae isolated from clinical samples in a tertiary care hospital, Klaten, Indonesia. In: BMC Proceedings BioMed Central, pp. 1-8.‏
Niveditha, S, Pramodhini, S, Umadevi, S, Kumar, S & Stephen, S 2012, The isolation and the biofilm formation of uropathogens in the patients with catheter associated urinary tract infections (UTIs). Journal of Clinical and Diagnostic Research, 6: 1478.
Peletiri, C, Matur, BM, Ihongbe, JC & Okoye, M 2012, The effect of Azadirachta indica (Neem Tree) on human plasmodiasis: the laboratory perspective. Journal of Medical Sciences, 2: 13-17.
Pulit-Prociak, J, Chwastowski, J, Kucharski, A & Banach, M 2016, Functionalization of textiles with silver and zinc oxide nanoparticles. Applied Surface Science, 385: 543-553.
Rao, KB 2012, Novel microbial route to synthesize ZnO nanoparticles using Aeromonas hydrophila and their activity against pathogenic bacteria and fungi. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 90: 78-84.‏
Reddy ARN, Srividya L 2018, Evaluation of in vitro cytotoxicity of zinc oxide (ZnO) nanoparticles using human cell lines. Journal of Toxicology and Risk Assessment, 4: 009. doi.org/10.23937/2572-4061.1510009
Saadi Al-Baer, A & Hussein, AA 2017, Isolation and identification of Escherichia coli producing cytosine deaminase from Iraqi patients. International Journal of Advanced Research in Biological Sciences, 4: 1-6.
Saputrais, YY 2017, Biosynthesis and characterization of ZnO nanoparticles using the aqueous leaf extract of Imperata cylindrica L. In: IOP Conference Series: Materials Science and Engineering, IOP Publishing, p. 012004.‏
Singh, RP, Shukla, VK, Yadav, RS, Sharma, PK, Singh, PK & Pandey, AC 2011, Biological approach of zinc oxide nanoparticles formation and its characterization. Advanced Materials Letters, 2: 313-317.
Sirelkhatim, A, Mahmud, S, Seeni, A, Kaus, NH, Ann, LC, Bakhori, SK, Hasan, H & Mohamad, D 2015, Review on zinc oxide nanoparticles: Antibacterial activity and toxicity mechanism. Nano-micro letters, 7: 219-242.
Sturikova, H, Krystofova, O, Huska, D & Adam, V 2018, Zinc nanoparticles and plants. Journal of hazardous materials, 349: 101-110.
Thakur, S, Neogi, S, Ray, AK 2021, Morphology-Controlled Synthesis of ZnO Nanostructures for Caffeine Degradation and Escherichia coli Inactivation in Water. Catalysts, 11: 63.‏
Yusof, HM, Mohamad, R & Zaidan, UH 2019, Microbial synthesis of zinc oxide nanoparticles and their potential application as an antimicrobial agent and a feed supplement in animal industry: A review. Journal of Animal Science and Biotechnology, 10: 1-22.