A skeletochronological study of age in the Caspian pond turtle, Mauremys caspica caspica (Testudines: Geoemydidae) in Golestan Province, Iran

Document Type: Research Paper

Authors

Department of Biology, Faculty of Sciences, Golestan University, Gorgan, Iran

Abstract

We estimated age of the Caspian pond turtle, Mauremys caspicacaspicain Golestan Province with the skeletochronological method, using specimens collected by nets in natural habitats such as ponds and fish farms. In adult M. c. caspica, lines of arrested growth (LAGs) were clearly discernable in the phalangeal and fibula bone cross-sections. The median age of M. c. caspica in the examined Golestan Province population(n = 22) was calculated 9.6 years (4-12) in males (n = 10), and 10.16 years (4-13) in females (n = 12) for the first time. The maximum straight carapace length (SCL2) was in the range of 83.74–221.41 (mean ± SD = 178.39 ± 48.89) mm in males and 103.02–236.84 (193.52 ± 41.32) mm in female specimens. Significant correlation was found between body size and age (p < 0.001). No difference was found in age composition between the sexes. Our study showed that skeletochronological method can be successfully applied to the Caspian pond turtle based on the clear arrest of growth in the hibernation period.

Keywords


A skeletochronological study of age in the Caspian pond turtle,Mauremys caspica caspica(Testudines: Geoemydidae) in Golestan Province, Iran

 

Mahsa Yazarloo, Haji Gholi Kami*, Ali Akbar Bagherian Yazdi

                       

Department of Biology, Faculty of Sciences, Golestan University, Gorgan, Iran

 

* Corresponding author’s E-mail: hgkami2000@yahoo.com

ABSTRACT

We estimated age of the Caspian pond turtle, Mauremys caspicacaspicain Golestan Province with the skeletochronological method, using specimens collected by nets in natural habitats such as ponds and fish farms. In adult M. c. caspica, lines of arrested growth (LAGs) were clearly discernable in the phalangeal and fibula bone cross-sections. The median age of M. c. caspica in the examined Golestan Province population(n = 22) was calculated 9.6 years (4-12) in males (n = 10), and 10.16 years (4-13) in females (n = 12) for the first time. The maximum straight carapace length (SCL2) was in the range of 83.74–221.41 (mean ± SD = 178.39 ± 48.89) mm in males and 103.02–236.84 (193.52 ± 41.32) mm in female specimens. Significant correlation was found between body size and age (p < 0.001). No difference was found in age composition between the sexes. Our study showed that skeletochronological method can be successfully applied to the Caspian pond turtle based on the clear arrest of growth in the hibernation period.

Key words: Age, Caspian pond turtle, Golestan Iran, LAGs, Mauremys caspica,Skeletochronology.

INTRODUCTION

The Caspian pond turtle, Mauremys caspica (Gmelin 1774), is belonging to family Geoemydidae. This freshwater turtle is widespread throughout the Middle East (Yadollahvand & Kami 2014). Three subspecies were reported in Iran. M. c. caspica (Gmelin 1774) is widely distributed in Golestan, Mazandaran, Guilan, Ardabil, East and West Azarbaijan provinces; M. c. ventrimaculata in Fars and Esfahan provinces and M. c. siebenrocki in Bushehr, Kordestan, Kermanshah, Lorestan, Ilam, Khuzestan, Fars and Chahar Mahal Va Bakhtiari provinces (Fritz & Wischuf 1997; Kami et al. 2006; Fritz et al. 2007; Rastegar-Pouyani et al. 2008; Safaei-Mahroo et al. 2015).

There is limited information on age structure, age at maturity, growth, and longevity of turtle populations.

The ability to relate age to reproductive effort is integral to studies of population dynamics, which in turn can inform management strategies for those organisms considered at risk (Chaloupka & Musick 1997; Heppell et al. 2003a). Techniques used to estimate the age of turtles include analysis of body size (Frazer & Ehrhart 1985), weighing eye lenses, counting growth zones on plastral scutes (Gibbons 1983), and skeletochronology (Zug 1990; Klinger & Musick 1992; Thomas et al. 1997).

In turtles and tortoises, there are three main approaches to estimate age: skelotochronology (Zug et al. 1986), growth models (Carr & Goodman 1970) and growth ring counts (hereinafter GRC). Each one of the methods has its pros and cons (Curtin et al. 2008; Rodríguez-Caro et al. 2015).

Three common methods can be employed to directly validate the annual deposition of skeletal growth marks: the study of known-age animals, mark-recapture studies, and mark-recapture studies that incorporate fluorescent marking. All of these methods have been applied to turtles (Castanet & Cheylan 1979; Coles et al. 2001; Snover et al. 2007b). Although skeletochronology can provide reliable data about age, it is an invasive method which just can be used with death animals and also shows some biases (Curtin et al. 2008; Rodríguez-Caro et al. 2015). Bone formation and remodeling rates are hormonally controlled and synchronized to circadian patterns (Simmons 1992).

Parathyroid hormone (PTH), calcitonin, and vitamins A, C, D, and K have been found to influence rates of bone formation and remodeling (Narbaitz et al. 1991). In temperate climates, each year of growth during the warm season, and the subsequent slowing of growth in hibernation, commonly called Lines of Arrested Growth (LAGs), can be counted for age determination (Zivari & Kami 2017). However, there is substantial evidence that the spring surge in growth rates is also under endogenous control, such that animals maintained in captivity also demonstrate this pattern (Snover & Rhodin 2007). LAGs are generally very clear and easily studied, as suggested by Kleineberg & Smirina (1969), the first convergence in the distance between the LAGs was considered as the sign of reaching sexual maturity.

Hard bone tissues cannot grow through internal expansion, but rather they grow by appositional processes (on periosteally derived cortical bone) with the deposition of new tissue on the surface together with endosteal resorption (Enlow 1969). This process of resorption results in the loss of the innermost (earliest) growth marks and is a serious limitation in estimating age using skeletochronology. While not a serious issue for shorter-lived amphibians and reptiles, it is especially problematic in long-lived turtles, and the problem is noted to be extreme in age-estimate studies of marine turtles (Snover & Hohn 2004; Snover et al. 2007 b).

Freshwater turtles were the first turtles to have skeletal growth marks recognized in their long bones (Mattox 1936; Suzuki 1963; Enlow 1969). Growth parameters of Emys orbicularis and Mauremys rivulata in Mediterranean Turkey were estimated with skeletochornological method (Ciecek et al. 2016). There is little information on skeletochronological studies of Iranian reptiles.

This research is the first study on skeletochronology of the Caspian pond turtle, M. c. caspica in Iran. Our aim were to determine age of M. c. caspica in Golestan Province, Iran, comparing the age structure and body size,  attaining the correlation between age and SCL, and understanding some life history traits of the freshwater turtle. These data are an indispensable prerequisite for designing any conservation strategies for the endangered populations in Iran.

 

MATERIALS AND METHODS

During morphometric study of Caspian pond turtle, 112 live specimens were collected from 14 stations in aquatic habitats including rivers, ponds, pools and fish farms in Golestan Province, Northern Iran, during 2016-2017 by hand and net. Seventeen dead specimens were also found in addition to live specimens. These dead materials and five previously-fixed specimens (10 males, 12 females) were studied to estimate the age structure of turtles. Phalangeal and fibula bones were dissected and removed from these 22 specimens.

The maximum straight carapace length (SCL) and maximum plastron length (PL) were measured using digital caliper to the nearest 0.1 mm. Sexes were determined by visual observation of morphological characters. The bones were fixed in 10% formalin and decalcified by 10% (v/v) chloric acid for 2-4 h. According to the size of bone, processed in paraffin block preparation (for 1.5 h), tissues were embedded in small paraffin block. Serial sections (7, 10, and 15 µm) were prepared using a rotary microtome, and all sections were stained with hematoxylin and eosin (H & E), then examined under a light microscope (Zivary & Kami 2017).

For each bone, we selected a minimum of three cross-sections at the mid-diaphyseal level, with the smallest marrow cavity (Cicek et al. 2016). The number of LAGs was counted in the periosteal bone. In addition, the skeletochronological data were compared with growth rings using the largest plastral scutes (generally pectoral or abdominal) (Germano & Bury 1998) and the central carapacial scutes, by two observers (M.Y. and H.K.), and the lower number was recorded.

Age classes and SCL were normally distributed (Shapiro-Wilk test, P > 0.01), thus allowing comparisons using parametric tests (t - test).

We used Spearman’s rank correlation to examine the relationship between age and SCL. Data analysis was performed using SPSS 24 and Excel 2010 (Microsoft Office), and interpreted at   𝛼 = 0.05.

 

 

RESULTS

In all examined sections, lines of arrested growth) LAGs (, were clearly observed in the cross-sections (Fig. 1).  In M. c. caspica, the mean SCLwas 193.52 mm (range = 103.02–236.84 mm; N = 12; SD = 41.32) for females, and 178.39 mm (range = 83.74–221.41 mm; N = 10; SD = 48.89) for males. The character SCL was larger in females than in males (Table 1).

 

 

Fig. 1. Cross-section of phalange of M. c. caspica, Arrows: line of arrested growth (LAGs); m.c. = marrow cavity. (A) Phalangeal bone cross - section in female (SCL= 206.34 mm), 10 LAG, 10 um, 10x and (a) 20x. (B) Phalangeal bone cross section in male (SCL = 194.54 mm), 9 LAG, 7 um, H & E.

 

Mean plastron length (PL) was 183.25 mm (range = 99.22–219.94 mm; SD = 27.05) in females, and 155.48 mm (range = 75.6–186.34 mm; SD = 38.43) in males, hence PL is larger in females than in males. The marrow cavity was largely or entirely covered by the cancellous bone. The cancellous bone was very irregular and have small to medium trabeculae. Lines of arrested growth were clearly seen in compact bone on its outer surface. In all phalangeal and fibula cross-sections, well-defined LAGs were observed in M. c. caspica. The LAGs were generally in the form rings, located outside the cancellous bone. The first LAG was partially or completely resorbed by endosteal resorption in some individuals. The age range of M. c. caspica was between 4 and 12 years in males (mean = 9.6) and between 4 and 13 years in females (mean = 10.16) (Fig. 2). Both SCL and age showed normal distribution (Shapiro-Wilk test, p > 0.01).

No statistically significant difference was observed in the age structures between the sexes (Mann-Whitney U test; p = 0.46, U= 49). The Spearman’s rank correlation showed significant correlation between SCL and age (Spearman’s correlation coefficient, r = 0.86, p < 0.001, Fig. 3).

Skeletochronological study of age was compared with growth ring counts, revealing only a few numbers of LAGs and growth rings on carapace and plastron scutes, conformed to the age of individuals.

Our results demonstrated that growth ring counts were identical to skeletochronological study of age in 13% (2 females,1 male), while were higher than those in 9% (1 female, 1 male) specimens. In other specimens, growth ring counts were lower than skeletochronological counts.

 

Table 1. Age and SCL of M. c. caspica. Values are mean ± standard deviation and sample size (n = Number of individuals).

Individuals

SCL (mm)

Age (years)

Males (n = 10)

Females (n = 12)

178.39

193.52

9.6

10.16

                                           SCL = Straight carapace length

                                                             mm = Millimeter

                                                             n= Number of individuals

 

 

Fig. 2.  Age frequency of M. c. caspica (adult specimens).

 

Fig. 3. Correlation between age classes and SCLin M.c. caspica female and male specimens from Golestan Province, Iran.

DISCUSSION

Fluctuating individual numbers and alterations in population structures often become only apparent during long-term studies. Photographic documentation of distinctive individual characters (pattern of front legs, head sides, carapace, scars, and anomalies) proved to be a powerful, non-invasive method for identifying small numbers of adult freshwater turtles (< 50) (Schneeweiss 2004). Auer & Taşkavak (2004) estimated the age of E. orbicularis and M. rivulata using plastral growth rings. This method had some disadvantages such as erosion of the growth rings. The missing rings caused false calculations in age determination studies (Germano & Bury 1998).

The body size and growth rates of freshwater turtles could be affected by several environmental factors, including thermal regimes (Germano & Bury 2009). The growth and maturation duration of freshwater turtles are influenced by ambient air and water temperatures (Brown et al. 1994), often vary with geographic latitude (Iverson et al. 1993). Thus, it is crucial to support their regeneration by conservation measures (Paul 2003).

In our results, ring production was cyclical, assuming one ring per year only in 13% of specimens. In accordance with Wilson et al. (2003), we were identified four critical sources of mismatches, obscuring the accuracy of GRC as an age estimator. These sources can be divided into two groups: At first, deposition of rings may not follow the usual assumed one ring per year (1:1 ratio), 1- because some individuals may deposit more than one rings in the same season during periods or in habitats with good resources availability (Aresco & Guyer 1998; Berry 2002). 2- Some individuals may stop growing, and may not even deposit any ring for years (Turner et al. 1987). Secondly, even if ring deposition follows a 1:1 ratio, other factors may negatively affect the accuracy of the technique: 3- scutes worn-out from friction may make it impossible to discern individual rings, thus age may be underestimated (Litzgus & Brooks 1998; Berry 2002); and 4- identification of rings requires an observer’s arbitrary decision, and repeated measures may yield different results (Germano & Bury 1998; Rodríguez-Caro et al. 2015).

Skeletochronology and the scute growth line counts from dead turtles can serve as supporting evidence of the annual nature of the two methods (Castanet & Cheylan 1979). Even when the scute growth line counts accurately estimate age, an advantage of skeletochronology over these counts appears with older adult animals. Growth lines of scutes could not be counted in our older specimens. As growth slows to nearly immeasurable rates in older animals, growth lines can no longer be differentiated on scutes (Snover & Rhodin 2007), hence only minimum ages can be estimated. On the other hand, a disadvantage is also found in the study of skeletochronology. One or two LAGs may not be counted due to the expansion of cancellous bone and resorption of secondary bone (Chinsamy & Valenzuela 2008).

The median age of M. rivulata is 11 years in Southeastern Europe and the Caspian region (Wischuf & Busack 2001). In Southern Anatolia population, recorded longevity is 12 years in two freshwater species, M. rivulata and E. orbicularis(Auer & Taşkavak 2004). In our results, the mean SCL was 193.52 mm for females and 178.39 mm for males. The median age of M. c. caspica was calculated 9.6 years (range = 4-12) for males, and 10.16 years (range = 4-13) for females. No statistically significant difference was observed in the age between the sexes (Mann-Whitney U test; P = 0.46, U = 49). Growth rings on carapace and plastral scutes could count up to 11 years; They were identical to skeletochronological counts in 3 specimens, were higher than those in 2 specimens, but were lower than those in 17 specimens. In Mediterranean Turkey population, the median age of M. rivulata was calculated 10 years (6-12) for males, and 10 years (range = 8-12) for females, while in E. orbicularis, 8 years (range = 5-9) for males, and 10 years (range = 5-12) for females. No statistically significant difference in age composition was observed between the sexes for M. rivulata (U test, p < 0.97) and E. orbicularis (U test, p < 0.03). Growth rings on plastral scutes could easily count up to 11 years for E. orbicularis and 12 years for M. rivulata in Southern Anatolia (Çiçek et al. 2016).

In our results, female body size was larger than male body size that can be the result of importance of fecundity as a factor influencing body size in female turtles (Gibbons et al. 1982; Brophy 2006; Yazarloo et al. 2017).

Given the vulnerable and endangered status of turtle populations worldwide, the need for age data to accurately characterize the status of these species is vital for conservation efforts (Heppell et al. 2003a, b).

 

ACKNOWLEDGMENTS

We thank Mrs. Najmeh Okhli for their help in sample collection from Golestan University.

 

Aresco, MJ & Guyer, C 1998, Efficacy of using scute annuli to determine growth histories and age of Gopherus polyphemus in southern Alabama. Copeia, 1094-1100.

Auer, M & Taşkavak, E  2004, Population structures of syntopic Emys orbicularis and Mauremys rivulata in western Turkey. Biologia Bratislava, 59: 81-84.

Avens, L & Goshe, LR 2007, Comparative skeletochronological analysis of Kemp’s ridley (Lepidochelys kempii) and loggerhead (Caretta caretta) humeri and scleral ossicles. Marine Biology, 152: 1309-1317.‏

Berry, KH 2002, Using growth ring counts to age juvenile desert tortoises (Gopherus agassizii) in the wild. Chelonian Conservation and Biology, 4: 416-424.

Brophy, TR 2006, Allometry and sexual dimorphism in the snail-eating turtle Malayemys macrocephala from the Chao Phraya River basin of Central Thailand. Chelonian Conservation and Biology, 5: 159-165.‏

Brown, GP,  Bishop,  CA & Brooks,  RJ 1994, Growth rate, reproductive output, and temperature selection of snapping turtles in habitats of different productivities. Journal of Herpetology, 28: 405-410.

Buchanan, CR & Preece,  MA 1991, Hormonal control of bone growth, in Bone, Bone Growth-A, B.K. Hall (ed.), Boca Raton, FL: CRC Press, 53-90.

Carr, A & Goodman, D 1970, Ecological implications of size and growth in Chelonia. Copeia, 783-786.

Castanet, J & Cheylan, M 1979, Les marques de croissance des os et des ecailles comme indicateurde l’age chez Testudo hermanni et Testudo graeca (Reptilia, Chelonia, Testudinidae). Canadian Journal of Zoology, 57: 1649-1665.

Castanet, J, Francillon-Vieillot, H, Meunier, FJ & De Ricqles, A 1993, Bone and individual aging. In: BBK Hall (Ed.) Bone, bone growth B. CRC Press, Boca Raton, FL, 7:  245-283.

Castanet, JH, Meumier, FJ & de Ricqles, A 1977, L’enregistrement dela croissance cyclique parle tissue osseux chez les vertebres poikilothermes: Donnees comparatives et essai de synthese. Bulletin Biologique de la France et de la Belgique, 111: 183-202.

Chaloupka, MY & Musick, JA 1997, Age, growth, and population dynamics. In: P, Lutz & J Musick  (Eds.) The biology of sea turtles, CRC Press, Boca Raton, FL, 233-276.

Çiçek , K, Kumaş, M, Ayaz, D & Tok, CV  2016,  A skeletochronological study of age, growth and  longevity in two freshwater turtles, Emys orbicularis and Mauremys rivulata, from Mediterranean Turkey (Reptilia: Testudines). Zoology in the Middle East, 62: 29-38.

Chinsamy, A & Valenzuela, N 2008, Skeletochronology of the endangered side-neck turtle, Podocnemis expansa. South African Journal of Science, 104: 311-314.

Coles, WC, Musick, JA & Williamson, LA 2001, Skeletochronology validation from an adult loggerhead (Caretta caretta). Copeia, (1): 240-242.

Curtin, AJ, Zug, GR, Medica, PA & Spotila, JR 2008, Assessing age in the desert tortoise Gopherus agassizii: testing skeletochronology with individuals of known age. Endangered Species Research, 5: 21-27.

Germano, DJ & Bury, RB 1998, Age determination in turtles: evidence of annual deposition of scute rings. Chelonian Conservation and Biology, 3: 123-132.

Germano, DJ & Bury, RB 2009, Variation in body size, growth, and population structure of Actinemys marmorata from lentic and lotic habitats in Southern Oregon. Journal of Herpetology, 43: 510-520.

Gibbons, JW, Gereene, JL & Patterson, KK 1982, Variation in reproductive characteristics of aquatic turtles. Copeia, 4: 776-784.

Enlow, DH, Gans, C, Bellairs d’A, Parsons (eds.) TS 1969, The bone of reptiles in biology of the Reptilia. London: Academic Press, 1: 45-80.

Francillon-Vieillot, H, de Buffrenil, V, Castenet, J, Geraudie, J, Meunier, FJ, Sire, JY, Zylberberg, L & de Ricqles, A 1990, Microstructure and mineralization of vertebrate skeletal tissues, In: Skeletal bio-mineralization: patterns, processes and evolutionary trends, JG Carter (Ed.), New York: Van Nostrand Reinhold, 1: 471–530.

Frazer, NB, Andl, M & Ehrhar, T 1985, Preliminary growth models for green, Chelonia mydas, and loggerhead, Caretta caretta turtles in the wild. Copeia 1: 73-79.

Fritz, U, Ayaz, D, Boschbom, J, Kami, HG, Mazanaeva, LF, Aloufi, AA, Auer, M, Rifai, L, Silic, T & Hundsdorfer AK 2007, Go east: Phylogeographies of Mauremys caspica and M.rivulata_discordance of morphology, mitochondrial and nuclear genomic markers and rare hybridization. Journal of Evolutionary Biology, 21: 527-540.

Fritz, U & Wischuf, T 1997, Zur Systematik westasia tischsü dosteuropäischer Bachschildkröten (Gattung Mauremys) (Reptilia: Testudines: Bataguridae). Zoologische Abhandlungen, Staatliches Museum fürTierkunde Dresden, 49: 223-260 (In German).

Germano, DJ & Bury, RB 1998, Age determination in turtles: evidence of annual deposition of scute rings. Chelonian Conservation and Biology,3: 123-132.

GIBBONS, JW 1983, Reproductive characteristics and ecology of the mud turtle, Kinosternon subrubrum (Lacepede). Herpetologica, 39: 254-271.

Gmelin, SG 1774, Reise durch Rußland zur Untersuchung der drey Natur-Reiche. Vol. 3. Dritter Theil. Reise durch das nordliche Persien, in den Jahren 1770, 1771, bis im April 1772. St. Petersburg: Kayserliche Academie der Wissenschaften [In Germany].

Heppell, SS, Snover, ML & Crowder, LB 2003a, Sea turtle population ecology. In: PL, Lutz, JA,  Musick & J, Wyneken  (Eds.) The Biology of Sea Turtles, 2: 275-306.

Heppell, SS, Crowder, LB, Crouse, DT, Epperly, SP& Fraser, NB 2003b, Population models for Atlantic loggerheads: past, present, and future. In: AB, Bolten & BE, Witherington (Eds.) Loggerhead sea turtles. Smithsonian Books, Washington, DC, 255-273.

Iverson, JB, Balgooyen, C, Burd, KK & Lyddan, KK 1993, Latitudinal variation in egg and clutch size in turtles. Canadian Journal of Zoology, 71: 2448-2461.

Kami, HG, Hojati, V, Pashaee Rad, S & Sheidaee, M 2006, A biological study of the European pond turtle, Emys orbicularis persica, and the Caspian pond turtle, Mauremys caspica caspica, in the Golestan and Mazandaran provinces of Iran. Zoology in the Middle East, 37: 21-28.

Klinger, RC & Musick, JA 1992, Annular growth layers in juvenile loggerhead turtles (Caretta caretta), Bulletin of Marine Science,51: 224-230.

Lambert, MRK 1982, Studies on the growth, structure and abundance of the Mediterranean spur-thighed tortoise, Testudo graeca in field populations. Journal of Zoology, 196: 165-189.

Leuteritz, TEJ & Gantz, DT 2013, Sexual dimorphism in radiated tortoises (Astrochelys radiata), turtles on the Brink in Madagascar. Chelonian Research Monographs, 6: 105-112.

Leuteritz, TEJ & Ravolanaivo, R 2005, Reproductive ecology and egg production of the radiated tortoise (Geochelone radiata) in southern Madagascar. African Journal of Zoology, 40: 233–242.

Litzgus, JD & Brooks, RJ 1998, Testing the validity of counts of plastral scute rings in spotted turtles, Clemmys guttata. Copeia, 222-225.

Mattox, NT 1936, Annular rings in the long bones of turtles and their correlation with size, Trans. IL. State Acad. Sci., 28: 255–256.

McCreary, B, Pearl, CA, Adams, JM 2008, A protocol for aging Anurans using skeletochronology. US Geological Survey Open-File Report, 1209.

Narbaitz, R 1991, Effects of vitamins A, C, D, and K on bone growth, mineralization, and resorption. In: Bone, bone metabolism and mineralization, BK, Hall (Ed.), Boca Raton, FL: CRC Press, 4: 141–170.

PAUL, R 2003, Untersuchung zur Raumnutzung und Durchfu¨hrungeiner  Gefährdungsanalyseaneiner Population der Europäischen Sumpfschildkr¨ote (Emys orbicularis, Linnaeus, 1758) in NO Deutschland. PhD Thesis, Universität Hamburg, 106 [In Germany].

Perälä, J 2001, Morphological variation among middle eastern Tstudo graeca Linnaeus, 1758 (Sensu lato), with a focus on taxonomy. Chelonii, 3: 78-108.

Rastegar-Pouyani, N, Kami, HG, Rajabizadeh, M, Shafiei, S & Anderson, SC 2008, Annotated checklist of amphibians and reptiles of Iran. – Iranian Journal of Animal Biosystematics (IJAB), 4: 43-66.

Rodríguez-Caro, R, Graciá, E, Dos Santos, RM, Anadón , JD & Gimenez, A  2015, One scute ring per year in Testudo graeca? A novel method to identify ring deposition patterns in tortoises. Acta Herpetologica, 10: 77-84.‏

Safaei-Mahroo, B, Ghaffari, H, Fahimi, H, Broomand, S, Yazdanian, M, Najafi Majd, E, Hosseinian, SS, Yousefkhani, E, Rezazadeh, MS, Hosseinzadeh, R, Nasrabadi, M, Rajabizadeh, M, Mashayekhi, A, Motesharei, Naderi  A & Kazemi, SM 2015, The herpetofauna of Iran: checklist of taxonomy, distribution and conservation status. Asian Herpetological Research, 6: 257-290.

Schneeweiss, N 1998, Status and protection of the European pond turtle (Emys o. orbicularis) in Brandenburg, Northeast Germany. In:  U, Fritz, U, Joger, R, Podloucky & J, Servan (Eds.) Proceedings of the EMYS Symposium Dresden 96, Mertensiella 10, 219-226.

Schneeweiss, N 2004, Age structure of relict populations of the European pond turtle (Emys orbicularis) at the northwestern boundary of its range. Biologia, Bratislava, 59: 123-129.

Snover, ML 2002, Growth and ontogeny of sea turtles using skeletochronology: Methods, validation, and application to conservation, PhD Dissertation, Duke University, Durham, NC.

Snover, ML, Avens, L & Hohn, AA 2007a, Back-calculating length from skeletal growth marks in loggerhead sea turtles Caretta caretta. Endangered Species Research, 3: 95-104.

Snover, ML & Hohn, AA 2004, Validation and interpretation of annual skeletal marks in loggerhead (Caretta caretta) and Kemp’s ridley (Lepidochelys kempi) sea turtles. Fish Bull, 102: 682-692.

Snover,  ML, Hohn, AA, Goshe,  L R & Balazs, GH  2011, Validation of annual skeletal marks in green sea turtles Chelonia mydas using tetracycline labeling. Aquatic Biology, 12: 197-204.‏

Snover, ML, Hohn, AA, Crowder, LB & Heppell, SS 2007b, Age and growth in Kemp’s Ridley Sea turtles: Evidence from mark-recapture and skeletochronology. In: Biology and conservation of Ridley Sea Turtles, PT, Plotkin (Ed.), Baltimore, MD: Johns Hopkins University Press, 89-105.

Snover, M L, Hohn, AA, Crowder, LB & Macko, SA 2010, Combining stable isotopes and skeletal growth marks to detect habitat shifts in juvenile loggerhead sea turtles Caretta caretta. Endangered Species Research, 13: 25-31.

Snover, ML, Rhodin, AG 2007, Comparative ontogenetic and phylogenetic aspects of chelonian chondro-ossious growth and skeletochronology. The biology of turtles, CRC Press, Boca Raton.‏

Simmons, DJ 1992, Circadian aspects of bone biology, In: Bone, bone growth-A. BK, Hall (Ed.), Boca Raton, FL: CRC Press,6:  91-128.

Suryan, RM, Saba, VS, Wallace, BP, Hatch, SA, Frederiksen, M & Wanless, S 2009, Environmental forcing on life history strategies: Evidence for multi-trophic level responses at ocean basin scales. Progress in Oceanography, 81: 1-4.

Suzuki, HK 1963, Studies on the osseous system of the slider turtle. Annals of the New YorkAcademyof Sciences, 109: 351-410.

Terentev,  PV &  Chernov, SA 1949, Key to amphibians and reptiles, Third edition (Translated from Russian by the Israel program for scientific translation) Translated by L. Kochva, L, 195p.

Thomas, RB, Beckman, DW, Thompson, K, Buhlmann, KA , Gibbons, JW & Moll, DL 1997, Estimation of age for Trachemys scripta and Deirochelys reticularia by counting annual growth layers in claws. Copeia, 4: 842-845.‏

Tracy, CR 1995, Estimating age of desert tortoises (Gopherus agassizii) from scute rings. Copeia, 964-966.

Turner, FB, Medica, PA & Bury, RB 1987, Age-size relationship of desert tortoise (Gopherus agassizi) in southern Nevada. Copeia, 974-979.

Wilson, DS &Tracy, CR 2003, Estimating age of turtles from growth rings: a critical evaluation of the technique. Herpetologica, 59: 178-194.

Wischuf, T & Busack, SD 2001, Mauremys rivulata (Valenciennes in Bory de Saint-Vincent et al., 1833) – Ostmediterrane Bachschildkröte. Handbuch der Reptilien und Amphibien Europas, 3: 89-110.

Yadollahvand, R, Kami , HG, Mashroofeh, A & Bakhtiari, AR 2014, Assessment trace elements concentrations in tissues in Caspian Pond Turtle (Mauremys caspica) from Golestan province, Iran. Ecotoxicology and Environmental Safety, 101: 191-195.

Yazarloo, M & Kami, HG 2017, Sexual dimorphism and morphometric study of Caspian pond turtle, Mauremys caspica, (Testudines: Geoemydidae) in Golestan Province, southeast of the Caspian Sea. Caspian Journal of Environmental Sciences, 15: 321-334.

Zivari, S & Kami, HG 2017, Skeletochronological assessment of age in the Persian mountain salamander, Paradactylodon gorganensis (Clergue-Gazeau and Thorn, 1979) (Caudata: Hynobiidae) from Golestan Province, Iran.‏Caspian Journal of Environmental Sciences, 15: 75-84.

Zug, GR, Wynn, AH & Ruckdeschel, C 1986, Age determination of loggerhead sea turtles, Caretta caretta, by incremental growth marks in the skeleton. Smithsonian Contributions to Zoology, 427: 1-34.

ZUG, GR 1990, Age determination of long lived reptiles: some techniques for sea turtles. Annales des Sciences Naturelles, 11: 219-222.