In Vitro Inhibition of Growth in Saprolegnia sp. Isolated

Authors

1 O. Rafieyan*1, A. A. Darvishsefat2, S. Babaii1, A. Mataji1

2 A. Aghaei Moghaddam*1

Abstract

Saprolegnia is one of? the? ?most important agents decreasing the eggs survival rate in sturgeon hatcheries. There are some chemical substances for controlling the fungal infection of eggs. In this study, an attempt was made to introduce a ?germ negative bacterium, Pseudomonas aeroginosa (PTCC1430)(Persian Type Culture Collection) as a biocontrol??ling agent of? ?water mold. Saprolegnia was isolated from the eggs of some infected Persian sturgeon, Acipenser persicus? in a sturgeon hatchery and then ?was purified. P. aeroginosa was cultured in Potato dextrose Agar (PDB) media and then was prepared in 5 concentrations (103,104,105,106and107cfu.ml-1) while challenging with fungi in petri dishes under laboratory conditions. The results showed that by increasing the concentration of the bacteria in plates, ???hyphal growth of the fungi was reduced. The ?highest concentration of P. aeroginosa concentration (107) roughly stopped the ?fungi growth and the Minimum Inhibitory Concentration (MIC) was 104cfu.m-l. Results? in this study implied the potential?? of? P. aeroginosa (PTCC1430) as a biological agent in controlling saprolegniosis.

Keywords