Effects of LHRH-A2 and chlorpromazine (dopamine antagonists) on inducing spawning in Caspian Kutum, Rutilus frisii kutum, from the southwest of the Caspian Sea


1 Dept. of Fisheries Sciences, Faculty of Natural Resources, Islamic Azad University of Lahijan, Iran

2 Biology Dept., Science Faculty, kharazmi University,Tehran, Iran


Caspian kutum, Rutilus frisii kutum, is one of the most commercially important fish species in the Caspian Sea, but there are few reports about the endocrinology of induced spawning in this fish. In the present study, 54 individuals of female broodstocks of Kutum were studied for quantity and quality of propagation index, during March and April 2007. Hormone treatments include: LHRH-A2 (1 ?g kg-1bw; chlorpromazine (2.5 mg kg-1bw); LHRH-A2 + chlorpromazine (1?g kg-1bw+2.5 mg kg-1bw); CPE as a positive control (1 mg kg-1bw); Physiological saline as a negative control; and normal brood stocks without injection. For histological examinations, ovarian samples of non-ovulated females were fixed in bouin's fluid. The routine procedures of preparation of tissues were followed and the paraffin blocks were cut at 5-7 microns, stained with H&E and studied under light microscope. The results showed that the highest percentage of ovulated females belonged to group 1 without significant difference with positive control. There was a significant difference between ovulated females in the LHRH-A2, chlorpromazine and LHRH-A2 + chlorpromazine treatments (P<0.05), while no significant differences in other propagation indices were found in any of the hormone treatments (P>0.05). Chlorpromazine could not be a potent dopamine antagonist and the microscopic observations of ovary in non- ovulated brood stocks showed that the oocytes in these treatments were in the final phase of IV stage.


Abdoli, A. (1999) Inland Water Fishes of Iran. Nature and Wildlife Museum Publications. 377 p, 115-120.
Alok, D., Krishnan, T., Talwar, G. P. and Gary, L.C. (1993) Induced spawning of catfish, Heteropneustes fossilis (Bloch), Using D-Lys6, Salmon gonadotropin – releasing hormone analog. Aquaculture.115, 159-167.
Arbaci, M., Cegirgan, H., Sari, M. (2004) Induction of ovulation in ornamental common carp (Koi, Cyprinus carpio L.) using LHRHa ([D-Ser9 (tBu6), Pro9-Net]-LHRH) combined with haloperidol and carp pituitary extract. Aquaculture Research.35, 10-14.
Billard, R. (1990) The major carps and other cyprinids. In: Production of Aquatic Animals (Fishes) (ed. by C.E. Nash), pp. 21-55.
Elsevier science publication, Amsterdam, the Netherlands Biswas, S.P.1993. Manual of methods in fish biology. South Asian publisher. New Dehli, pp. 190.
Breton, B., Weil, C., Sambron, E., Zohar, Y. (1990) Effects of acute versus sustained administration of GnRH-a on GTH release and ovulation in the rainbow trout, Oncorhynchus mykiss. Aquaculture.91, 373-383.
Brzuska, E., Adamek, J. (1999) Artificial spawning of European catfish, Silurus glanis L.: stimulation of ovulation using LHRH-a, Ovaprim and carp pituitary extract. Aquaculture Research.30(1), 59-64
Dollery, C. (1991) Therapeutic Drugs. Volume 1. Edinburgh: Churchill Livingstone, 201-206. Donaldson, E.M. (1996) Manipulation of reproduction in farmed fish. Animal Reproduction science42, 381-392.
 Dorafshan, S., Majazi Amiri, B., Hajizadeh, A., Mostafavi, H. Paykan Heyrati, F. (2002) Induced proliferation of female rainbow trout Oncorhynchus mykiss using the synthetic hormone GnRHa. Iranian Journal of Fisheries. 3, 23-38.
 Dorafshan, S., Mostafavi, H., Mojazi Amiri, B. (2003) Induction of spawning in common carp (Cyprinus carpio), using pituitary extract and GnRH analogue in combination with domperidone. Iranian Journal of Biotechnology.1, 213-217.
Drori, S., Ofir, M., Sivan, B.L. Yaron, Z. (1994) Spawning induction in common carp (Cyprinus carpio) using pituitary extract or GnRH super active analogue combined with methoclopramide: analysis of hormone profile, progress of oocyte maturation and dependence on temperature. Aquaculture. 119:393-407.
40Effects of LHRH-A2 and chlorpromazine...
Erdahl, D.J., McClain, J. (1987) Effect of LHRHanalogue treatment on egg maturation (Ovulation) in lake trout brook stock. Progressive fish-culturist.49, 276-279
Fermin, C.A. (1991) LHRH-a and domperidon– induced oocyte maturation and ovulation in bighead carp, Aristichthys nobilis.Aquaculture.93, 87-94.
Gissis, A., Levavi-sivan, B., Rubinp-kedem, H., ofir, M., Yaron, Z. (1991) The effect of gonadoropin releasing hormone superlative analogue and dopamine antagonists on gonadotropin level and ovulation in tilapia hybrids. J. Aquacultu – Bamidgeh43, 123-136.
Glubokov, A.I., Motloch, N.N., Sedova, M.A. (1991) Effect of synthetic LHRH analogue and dopamine antagonists on the maturation of bream, Abramis brama. Aquaculture95, 373-377.
Ghobadi, Sh. (2004) Comparative study on effects of LHRH and dopamine antagonists (Pimozide) sexual maturation in common carp maturation (Cyprinus carpio). MSc Thesis. Islamic Azad University of Lahijan, Iran. 60p.
Haraldson, H., Sveinsson, T., Skulason. S. (1993) Effects of LHRH-a treatments upon the timing of ovulation & upon egg and offspring quality in arctic charr, Salvelinus alpinus. Aquaculture & Fisheries Management24, 145-150.
Kashani Sabet, A. R., Oryan, Sh., Bahmani, M. (2004) ovulation Induction in silver carp phytophage, Hypophtalmichthys molitrix by using of LHRH-A and its combination with dopamine antagonists (Metacloperamid and Dompridone). Journal of Fisheries of Iran. 3, 129-144.
Kazemi, R., Bahmani, M. (1998) Instructions for preparing and staining tissues for histological studies. International Institute of sturgeon Dr Dadman. 16 p.
Kouril, j., Barth, T., Hamackova, J., Flegel, M. (1986) Induced ovulation in tench, Tinca L. various LHRH synthetic analogues: Effect of site of administration and temperature. Aquaculture54: 37-44.
Kulikovsky, Z., Martin, F.J.B., Yaron, Z. (1996) A comparison of two spawning inducing agent for common carp. The Israeli Journal of Aquaculture, Bamidgeh48, 108-111.
Lee, C. S., Tamaru, C. S., Kelley, C. S., Banno, J.E. (1986) Induced spawning of milkfish, Chanos chanos, by a single application of LHRH – analogue. Aquaculture58, 87-98.
Lin, H.R., Chun, p., Van Der Kraak, G., Peter, R.E., Breton, B. (1986) Effects of [D-Ala6, Pro9- Net] – LHRH and catecholaminergic drugs on gonadotropin secretion and ovulation in the Chinese loach, Paramisgurnus dabryanus, General Comparative Endocrinology.64,389-395.
Lin, H.R.,Van Der Kraak, G., Zho, X-J., Liang, J.Y., Peter, R.E., Rivier, J.E., Vale, W.W. (1988) Effects of [D-Arg6 Trp7 Leu8 Pro9 Net]-luteinizing hormone-releasing hormone (sGnRH-A), and [D-Ala6 Pro9 NEt]-luteinizing hormone-releasing hormone (LHRH-A), in combination with pimozide or domperidone, on gonadotropin release and ovulation in the Chinese loach and Common carp. General Comparative Endocrinology.69, 31-40.
Linard, B., Bennani, S., Saligaut, C. (1995) Involvement of estradiol in a catecholamine inhibitory tone of gonadotropin release in the rainbow trout (Oncorhynchus mykiss). General Comparative Endocrinology.99, 192-196.
 Mylonas, C.C., Zohar, Y. (2001) Use of GnRHa-delivery systems for the control of reproduction in fish. Reviews in Fish Biology and Fisheries 10, 463-491.
Nandeesha, M.C., Das, S.K., Nathaniel, E., Verghese, T.J., shetty, H.P.C. (1989) Ovaprim a new drug for induced breeding of carps. Fishing chimes, 13-15.
Nazari, R. M. (1996) biology and artificial propagationof Silver carp,Hypophtalmichthys molitrix. Shilat Department in Bandar anzali, Iran. 94 p.
Nelson, J.S. (1976) Fishes of the world. New York, John wiley and Sons. Paykan Heyrati, F., Mostafavi, H., Mojazi Amiri, B., Hajizadeh, A., Dorafshan, S. (2001) Induced spermiation of rainbow trout (Oncorhynchus mykiss) using a GnRH analogue. Iranian Journal of Fisheries Science3, 95-108
Peter, R.E., Nahorniak, C.S., Sokolowsha, M., Chang, J.P., Rivier, J.E, Vale, W.W., King, J.A., Millar, R.P. (1985) Structure–activity relationship of Ahmadnezhad et al., 41mammalian, chicken and Salmon gonadotropin releasing hormone in vivo in goldfish. General Comparative Endocrinology.58, 231-242.
Peter, R.E., Lin, H.R., Van Der Kraak, G. (1988) Induced ovulation and spawning of cultured fresh water fish in china: advances in application of GnRH analogues and dopamine antagonists. Aquaculture74, 1-10.
Peter, R.E., Yu, K.L. (1997) Neuroendocrine regulation of ovulation in fishes: basic and applied aspects. Rev. fish Biology. Fish7,173-197.
Poosti, A.S., Marvdasti, S. (1999) Atlas of fish histology (normal and pathological forms), Tehran University Press. 328 p.
Rasekhifar, Kh. (1998) Effects of application of Baclofen (agonist receptor GABA - B) alone and in combination with LHRH - A Metacloperamid and the release of GTH-I in carp (Cyprinus carpio L.). MSc Thesis. University of Tarbiat Moalem, Tehran, Iran. 120 p.
 Razavi Sayad, B. (1995) Kutum fish. Fisheries Research Institute, Bandar anzali, Iran. 164 p. Szabo, T., Medgyasszay, C., Horvath, L. (2002) Ovulation induction in nase (Chondrosmoma nasus, Cyprinidae) using pituitary extract or GnRH analogue combined with domperidone. Aquaculture203, 389-395.
Van Der Kraak G., Donaldson E.M., Chang J.P. (1986) Dopamine involvement in the regulation of gonadotropin secretion in Coho Salmon. Canadian Journal of Zoology,64, 1245-1248.
Yaron, Z. (1995) Endocrine control of gametogenesis and spawning induction in the carp. Aquaculture129, 49-73.
Zohar, Y. (1989) Fish reproduction, its physiology and artificial manipulation. In: Fish Culture in Warm Water Systems, Problems &Trends (ed. By M.C. Shilo & S.H. Sargi), pp.65-119. CRC Press, Boca
Raton, FL, USA. Zohar, Y., Mylonas, C.C. (2001) Endocrine manipulation of spawning induction in cultured fish from hormone to gene. Aquaculture197, 99-139.