University of Guilan Caspian Journal of Environmental Sciences 1735-3033 23 5 2025 12 01 Protective effects of 1, 8-cineole (eucalyptol) against CCl4 induced hepatic oxidative damage in rats 1217 1224 8686 10.22124/cjes.2025.8686 EN Mehriban S. Baratova Candidate of Medical Sciences, Associate Professor - Head of the Department of Public Health, Healthcare Management, Bukhara State Medical Institute, Uzbekistan Ilhom A. Ulashov PhD in Veterinary Sciences, Senior Researcher at the Helminthology Laboratory, Veterinary Scientific Research Institute, Taylok region, Samarkand, Uzbekistan Zukhra Yakhshieva Doctor of Chemical Sciences, Professor, Jizzakh State Pedagogical University, Jizzakh, Uzbekistan Alimardon A. Egamberdiev Assistant, Department of "Engineering Graphics and Design Theory", "Tashkent Institute of Irrigation and Agricultural Mechanization Engineers", National Research University, Tashkent, Uzbekistan Botirbek Хoshimov Doctor of Philosophy (PhD Veterinary Science), Department of Agronomy, Navoi State University of Mining and Technologies, Navoi, Uzbekistan Umid A. Kodirov Teacher at the Department of Neurology, Faculty of Postgraduate Education, Samarkand State Medical University, Samarkand, Uzbekistan Ajiniyaz A. Eshmuratov Lecturer, Department of Art Science, Karakalpak State University named after Berdakh, Nukus, Republic of Karakalpakstan Klara X. Avilova Senior Teacher, Department of Pharmaceuticals and Chemistry, Faculty of Medicine, Alfraganus University, Tashkent, Uzbekistan Ikromjon I. Mamadoliev Kimyo International University in Tashkent, Tashkent, Uzbekistan Davlat Normurodov Professor Institute of Agrobiotechnology and Food Securite of Samarkand State University named after Sharof Rashidov, Jamboy district, Samarkand, Uzbekistan Journal Article 2025 04 22 Carbon tetrachloride (CCl₄) is a hepatotoxin which induce oxidative stress. Therefore, supplementation with antioxidants may be beneficial in mitigating its hepatotoxic effects. 1,8-Cineole (1,3,3-trimethyl-2-oxabicyclo [2.2.2] octane), commonly referred to as eucalyptol has various therapeutic properties. Hence, this research was conducted to evaluate the protective effects of eucalyptol against CCl₄ induced hepatic oxidative damage in rats. Thirty rats were divided randomly into the following three equal groups (n = 10): healthy control, CCl₄-poisoned group (1 mL/kg BW dissolved in olive oil (1:1 volume) and eucalyptol treated group (100 mg/kg/day). After treatment period (14 days), serum sample was prepared and liver was taken out for measurement of biochemical parameters including liver enzymes such as Alanine transaminase (ALT) and Aspartate transaminase (AST) as well as oxidative stress markers including Malondialdehyde (MDA), Glutathione (GSH), Catalase (CAT), Superoxide dismutase (SOD) and Glutathione Peroxidase (GPX). The results showed that following CCl₄ poisoning, a significant reduction in the levels of GSH, GPX, CAT and SOD was observed in the CCl₄-poisoned group compared to the control, as well as a significant elevation in the levels of AST, ALT and MDA was observed in the CCl₄-poisoned group in comparison with the control (<em>p</em> < 0.05). Furthermore, there was a significant increase in the levels of GSH, GPX, CAT and SOD in the eucalyptol treated group compared to the CCl₄-poisoned group. However, there was a significant decrease in the levels of AST and ALT in the eucalyptol-treated group compared to the CCl₄-poisoned group (<em>p</em> < 0.05). However, there was no significant in the MDA level in the eucalyptol-treated group compared to the CCl₄-poisoned one (<em>p</em> > 0.05). Eucalyptol may be effective in the prevention of CCl₄-induced liver damage through the reduction of oxidative stress, liver damage caused by elevated liver enzymes, and increasing antioxidant enzymes. https://cjes.guilan.ac.ir/article_8686_5525fd1656782d8809e931a0e8bb1964.pdf