University of Guilan Caspian Journal of Environmental Sciences 1735-3033 22 5 2024 12 01 Comparative analysis of antimicrobial properties of medicinal plants used in veterinary medicine 1043 1053 8071 10.22124/cjes.2024.8071 EN Gulzhaina Alpamyssova M. Auezov South Kazakhstan university, Shymkent, Kazakhstan Rakhat Pernebekova International Kazakh-Turkish University named after H.A.Yasavi, Turkestan city Nurzipa Saparbayeva M. Auezov South Kazakhstan university, Shymkent, Kazakhstan Zafar Khayitov Uzakovich Navoi State Pedagogical Institute, Navoi, Uzbekistan Toshmirza Rakhmonov Samarkand State University named after Sharof Rashidov, University Boulevard, 15, Samarkand, 703004, Uzbekistan Tillashaykhova Khosiat Azamatovna Head of the Department of General Psychology, Candidate of Psychology, Associate Professor, Tashkent State Pedagogical University named after Nizomi, Uzbekistan Sergey Kharchenko Associate Professor of Department of Electrical Machines and Electric Drives, Candidate of Technical Sciences, Associate Professor. Kuban State Agrarian University named after I.T. Trubilin, Krasnodar, Russia Khayitov Jamshid Komil Ugli Teacher of the Department of Fruits and Vegetables At the Urganch State University, Uzbekistan Abdiev Zafarali Toshtemirovich Tashkent State Agrarian University, 100140 Tashkent, Uzbekistan L. Suvonova Tashkent Institute of Irrigation and Agricultural Mechanization Engineers National Research University, Tashkent, Uzbekistan & Western Caspian University, Scientific researcher, Baku, Azerbaijan Shermatova Gulchehra Djumanazarovna Tashkent Institute of Irrigation and Agricultural Mechanization Engineers National Research University, Tashkent, Uzbekistan Panjiyeva Nasiba Narmaxmatovna Termiz State University, 190110 Termiz, Surkhandaryo, Uzbekistan Rasulov Ilkhom Inamovich Kokand State Pedagogical Institute 150700 Kokand, Fergana region, Uzbekistan Journal Article 2024 10 14 The surge in microorganisms' immunity to treatments has revitalized focus on herbal remedies in animal healthcare. The intent behind this research was to weigh the bacteria-thwarting efficacies of five medicinal plants commonly used in veterinary practice: Echinacea purpurea, Allium sativum, Matricaria chamomilla, Thymus vulgaris, and Calendula officinalis. Aqueous and ethanolic extracts of each plant were prepared and tested against four bacterial strains (Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Streptococcus pneumoniae) and two fungal strains (Candida albicans, Aspergillus fumigatus). Using MBC/MFC (minimum bactericidal/fungicidal concentration), MIC (minimum inhibitory concentration), and agar well diffusion tests, the germicidal efficacy was assessed. Phytochemical screening was performed to identify major bioactive compounds. All plant extracts exhibited antimicrobial activity, with T. vulgaris and A. sativum demonstrating the highest potency. Ethanolic extracts consistently outperformed aqueous extracts. Bacterial strains exhibited the greatest susceptibility to T. vulgaris's ethanol-based extract, with MIC readings ranging from 0.39 to 0.19 mg/mL. Phytochemical analysis revealed a high presence of terpenoids and phenolic compounds in the most effective extracts. The investigated curative botanicals demonstrate significant pathogen-inhibiting properties, particularly T. vulgaris and A. sativum. These findings suggest promising avenues for developing plant-based antimicrobial agents in veterinary medicine, potentially offering alternatives to synthetic antibiotics and addressing antimicrobial resistance concerns. https://cjes.guilan.ac.ir/article_8071_4fb5ab1d48fab6d8f50679265de08179.pdf